Abstract:
:Human gingival fibroblasts were cultured in vitro using as substrates an extracellular matrix (matrix) and polytetrafluoride (PTFE) membranes, which are used in guided tissue regeneration. To test the degree of biocompatibility of these membranes, the cellular proliferation and the accumulation of extracellular matrix (ECM) macromolecules were considered as parameters. The fibroblasts were cultured in vitro for 24 and 48 hours without serum on plastic, matrix, and PTFE membranes in the presence of 3H-thymidine, 3H-glucosamine, and 3H-proline to study the neo-synthesis of DNA, glycosaminoglycans (GAG), and collagen proteins, respectively. Studies on cell proliferation showed that fibroblasts grown on matrix membrane significantly increased 3H-thymidine incorporation, while fibroblasts grown on PTFE membrane decreased 3H-thymidine incorporation, compared to plastic used as a control. Moreover, the PTFE membrane induced a marked decrease of collagen and GAG accumulation both in the cellular and extracellular pool, while the matrix membrane provoked a decrease of the two macromolecules in the cellular pool and an increase in the extracellular one, compared to the control. The data we obtained demonstrate that matrix membranes are the most suitable to stimulate both cellular proliferation and ECM macromolecule accumulation.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Locci P,Calvitti M,Belcastro S,Pugliese M,Guerra M,Marinucci L,Staffolani N,Becchetti Edoi
10.1902/jop.1997.68.9.857subject
Has Abstractpub_date
1997-09-01 00:00:00pages
857-63issue
9eissn
0022-3492issn
1943-3670journal_volume
68pub_type
杂志文章abstract::Repeated measurements of attachment level appear to be important assessments in periodontal clinical trials, yet the lack of reliability for this assessment creates measurement error which in turn demands increased sample sizes or reduces the power of the test. A plastic occlusal stent has been developed as a fixed re...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1987.58.2.115
更新日期:1987-02-01 00:00:00
abstract:BACKGROUND:Several risk indicators for periodontal disease severity have been identified. The association of these factors with tooth loss for periodontal reasons was investigated in this cross-sectional comparative study. METHODS:All extractions performed in 21 general dental practice clinics (25% of such clinics in ...
journal_title:Journal of periodontology
pub_type: 杂志文章,多中心研究
doi:10.1902/jop.2005.76.11.1910
更新日期:2005-11-01 00:00:00
abstract::Gingival incisions were performed distal to each of the two lower incisors on 25 adult male guinea pigs. For every animal, electrosection with an electrosurgical scalpel was used on one side, and a conventional scalpel was used on the other. The surgical instruments in all cases were brought into direct contact with p...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1975.46.1.40
更新日期:1975-01-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.20-0040
更新日期:2020-08-06 00:00:00
abstract:BACKGROUND:Previous studies demonstrated significant variability in the histologic biologic width in periodontal health and mild periodontitis. The purpose of this study was to determine whether the previously established dimensions of the biologic width applied to subjects with severe, generalized, chronic periodontit...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2008.080066
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Some reports suggest that the prevalence and severity of gingival overgrowth (GO) is lower in patients taking tacrolimus (Tcr) than in those taking cyclosporin A (CsA). The present study was conducted to determine the prevalence and severity of GO in a group of renal transplant recipients treated with Tcr in...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050327
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:Gingival melanin depigmentation procedures are commonly associated with recurrence of pigmentation, which starts with migration of melanocytes from the adjacent free gingiva. The extent and rate of recurrence varies according to the treatment modalities used and duration of follow-up. The aim of this study i...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2012.120094
更新日期:2013-06-01 00:00:00
abstract:BACKGROUND:Oral lichen planus (OLP) is one of the most common oral mucosa disorders. OLP gingival involvement is very frequently observed and is characterized by a wide range of clinical appearance, symptomatology, and locations. The purpose of this study was to analyze the prevalence and clinical aspects of gingival l...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2007.060303
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:The use of various combinations of enamel matrix derivative (EMD) and grafting materials has been shown to promote periodontal wound healing/regeneration. However, the downstream cellular behavior of periodontal ligament (PDL) cells and osteoblasts has not yet been studied. Furthermore, it is unknown to what...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.110480
更新日期:2012-07-01 00:00:00
abstract:BACKGROUND:The purpose of this clinical study is to evaluate the radiographic bone remodeling, survival rate, and soft tissue health surrounding a variable-thread tapered implant immediately placed in extraction sites. METHODS:Sixty implants were placed in 55 patients at six centers according to a predetermined protoc...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1902/jop.2013.120638
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Platelet-rich plasma (PRP) has been promoted as a surgical adjunct to enhance hard and soft tissue wound healing. Although anecdotally reported to be of value, the results of controlled studies examining the added effects of PRP on surgical procedures have been mixed. The purpose of this study was to test th...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080626
更新日期:2009-06-01 00:00:00
abstract::This study was undertaken to determine the extent of in vitro penetration of E. coli endotoxin into the root cementum of periodontally healthy and diseased teeth. Freshly extracted teeth were washed in distilled water, scaled and divided into two groups of 16 teeth each. Nine diseased and five healthy teeth in the fir...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1982.53.6.368
更新日期:1982-06-01 00:00:00
abstract:BACKGROUND:Amelogenin is a major component of developing extracellular enamel matrix proteins and plays a crucial role during the formation of tooth enamel. In addition, amelogenins are suggested to exert biologic functions as signaling molecules through cell-surface receptors. The purpose of this study is to examine t...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.110408
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Prevalence of peri-implantitis is directly proportional to the time of functional loading. The aim of this retrospective study was to assess the survival and success rates as well as the incidence of peri-implantitis among patients with a history of periodontitis and among implants with 1 to 10 years of func...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.18-0715
更新日期:2019-12-20 00:00:00
abstract::HTR polymer was evaluated as a grafting material in periodontal osseous defects in 21 adult patients. Following initial preparation and pre-surgical documentation with standardized measurements, radiographs, and photographs, vertical osseous defects were treated with surgical open flap debridement alone (DEBR) or with...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1990.61.10.633
更新日期:1990-10-01 00:00:00
abstract::The present study demonstrated that hyperglycemic diabetic rats fed a low-protein (8%) diet maintained an alveolar bone height similar to controls; in contrast, those fed a standard protein diet (24%) had reduced alveolar bone height (P less than 0.05). Euglycemic diabetic and untreated control rats fed low-protein di...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1989.60.5.264
更新日期:1989-05-01 00:00:00
abstract::Extracts of corn and some nuts were found to ubiquitously stimulate both adult and newborn cord blood lymphocytes to transform and produce a factor chemotactic for monocytes. This indicates that corn and nuts contain a mitogen and are potential stimulators of the cellular immune response. The exposure of lymphocytes t...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1975.46.9.562
更新日期:1975-09-01 00:00:00
abstract:BACKGROUND:Chemically modified non-antimicrobial tetracyclines (CMTs) have been shown to inhibit pathologically elevated collagenase (and other matrix metalloproteinase, MMP) activity and bone resorption in vivo and in vitro. METHODS:In the current study, suboptimal doses of CMT-8 (a non-antimicrobial chemically modif...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2001.72.8.1069
更新日期:2001-08-01 00:00:00
abstract::This study compared demineralized-unicortical-ilium-strips (DUIS) and an expanded polytetrafluoroethylene (ePTFE) physical barrier in combination with decalcified freeze-dried bone allograft (DFDBA) for treatment of Class II mandibular furcations. Twenty patients with adult periodontitis and at least 2 furcation invas...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.8.803
更新日期:1996-08-01 00:00:00
abstract::Accurate diagnosis of periodontal destruction in the furcation region of multi-rooted teeth is a critical component of treatment planning, with different therapeutic approaches chosen based upon clinical determination of the severity of involvement. The current study assessed both vertical and horizontal depths of 274...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.7.649
更新日期:1994-07-01 00:00:00
abstract::Experimentation in animal models has created a basis for highly improved therapeutic procedures in reconstructive periodontal surgery. This symposium has discussed several animal models currently used; additional models have been described in recent literature. The presentations by Drs. Caton, Wikesjö, and Lynch illus...
journal_title:Journal of periodontology
pub_type: 评论,杂志文章
doi:10.1902/jop.1994.65.12.1169
更新日期:1994-12-01 00:00:00
abstract::Multiple periodontal lesions were the presenting features leading to a diagnosis of multiple myeloma in a 38-year-old male. Ulceration and bleeding from a rapidly growing retromolar myelomatous mass, multiple foci of alveolar bone destruction and cortical expansion together with multifocal skeletal destruction were th...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1990.61.2.132
更新日期:1990-02-01 00:00:00
abstract:BACKGROUND:Neopterin, a marker of cellular immune activation, is produced by human macrophages after induction by interferon gamma that is secreted by T lymphocytes. Neopterin concentrations in diverse body fluids have been reported to increase in parallel with bacteria in the clinical course of infections. Therefore, ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2002.73.7.720
更新日期:2002-07-01 00:00:00
abstract:BACKGROUND:Chronic exposure to occupational noise is reported to cause a wide range of health problems. But, there are no reports on studies investigating the association between noise exposure and periodontitis. The purpose of this study was to examine the relationship between occupational noise exposure and periodont...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.17-0484
更新日期:2018-04-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to longitudinally evaluate, over a 3-year period, the reduction of gingival recession through use of a subepithelial free connective tissue graft placed under a coronally advanced partial-thickness pedicle flap. METHODS:Twenty-one buccal recession defects (mean 3.67 mm; range 3...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.2002.73.12.1412
更新日期:2002-12-01 00:00:00
abstract:BACKGROUND:The number of immunocompromised patients is increasing as a consequence of successful organ transplantation. Placing dental implants in these patients has been questioned because of their increased risk for infections. The 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immu...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2004.75.6.909
更新日期:2004-06-01 00:00:00
abstract:BACKGROUND:This updated Cochrane systematic review (SR) evaluated the efficacy of different root coverage (RC) procedures in the treatment of single and multiple gingival recessions (GR). METHODS:We included randomized controlled trials (RCTs) only of at least 6 months' duration evaluating Miller's Class I or II GR (≥...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.19-0079
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND:This study evaluates the effects on clinical and biochemical parameters of Lactobacillus reuteri-containing probiotic supplementation adjunctive to initial periodontal therapy in patients with chronic periodontitis (CP). METHODS:Thirty patients with CP were included and divided into two groups. Every patien...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2015.140612
更新日期:2015-06-01 00:00:00
abstract:BACKGROUND:A subcategory of chronic neutropenia is chronic benign neutropenia, which is characterized by a prolonged non-cyclic neutropenia as the sole abnormality, with no underlying disease to which the neutropenia can be attributed. Chronic neutropenia is defined as a low absolute neutrophil count for >6 months. In ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.060076
更新日期:2006-11-01 00:00:00
abstract:BACKGROUND:Considering xenogeneic collagen matrix (CM) and enamel matrix derivative (EMD) characteristics, it is suggested that their combination could promote superior clinical outcomes in root coverage procedures. Thus, the aim of this parallel, double-masked, dual-center, randomized clinical trial is to evaluate cli...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2017.170126
更新日期:2017-12-01 00:00:00