Abstract:
:Demineralized bone matrix (DBM) is widely used in the repair of pathologies associated with skeletal defects and periodontal diseases. The present study was directed at establishing in vivo and in vitro models for a quantitative assessment of the osteoinductivity of DBM before clinical use. Athymic mice were used in an in vivo assay to overcome the species limitations (for human DBM) found in xenogeneic animal models. Calcium contents of explants, as an indicator of new bone formation, were assayed and expressed as a change in the weight percent calcium in the explant as compared to the weight percent of calcium in the implanted material. A total of 82 mice (2 implants per mouse) were used in this study. Significant amounts of new bone were induced in this animal model in response to implantation of DBM. Muscular implantation was found to be more osteoinductive (increases of 10.0 +/- 0.4 calcium weight percent of explant) than subcutaneous implantation (increases of 1.62 +/- 0.27 calcium weight percent of explant) and new bone formation in muscular implantation sites of athymic mice mimics endochondral bone formation. Between weeks 1 to 4, the weight of explanted materials did not significantly differ from the weight of the implanted material; however, by week 5 the explant weight began to increase. Calcium deposition over the 5 weeks of implantation increased in a nearly linear fashion. Consequently week 4 was chosen as the optimum time for explantation in the in vivo assay in that sufficient calcium levels had been achieved without a significant increase in explant dry weight. Aliquots of 10, 20, 30, and 40 mg per implantation site were used in dose response studies in the in vivo bioassay. Dose response curves with DBM exhibited maximal activity at the 20 mg DBM implant dose in the in vivo bioassay. An in vitro bioassay was also developed where human periosteal (HPO) cells were chosen because osteoprogenitor cells found in bone repair typically come from periosteal tissue. Alkaline phosphatase (ALP) activity in confluent cell cultures of HPO cells exposed to DBM, as an indicator of osteoblast induction, reached its highest level on day 5 of DBM treatment. Aliquots of 2, 5, 10, 20, 30, and 40 mg DBM per flask were chosen in dose response studies using the in vitro bioassay. These dose response studies with DBM revealed that quantities approximating 5 to 10 mg DBM in the in vitro model provided for maximal levels of ALP in cell extracts. A linear correlation (R2 = 0.7397) was demonstrated between the in vivo calcium remineralization assay and the in vitro ALP assay of osteoinductivity of DBM, suggesting that the in vitro assay can be used to quantitatively assess the osteoinductive potential of DBM where production and distribution of clinically usable DBM dictates rapid analysis.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Zhang M,Powers RM Jr,Wolfinbarger L Jrdoi
10.1902/jop.1997.68.11.1076subject
Has Abstractpub_date
1997-11-01 00:00:00pages
1076-84issue
11eissn
0022-3492issn
1943-3670journal_volume
68pub_type
杂志文章abstract:BACKGROUND:Combined periodontal regenerative technique (CPRT) is a surgical procedure that combines the use of barrier membranes with a filling material in the treatment of periodontal defects. The effectiveness of CPRT has been evaluated in many studies in comparison to GTR with membranes alone, but conflicting result...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.2002.73.2.158
更新日期:2002-02-01 00:00:00
abstract::Among the pseudocysts of the jaws, the traumatic bone cyst is known as an asymptomatic lesion often noted unintentionally during routine radiographic examinations. The lesion neither devitalizes the teeth within its borders, nor does it cause resorption of their roots. The well-demarcated traumatic bone cyst often pro...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1997.68.10.1019
更新日期:1997-10-01 00:00:00
abstract::The clinical periodontal status of 44 insulin-dependent diabetic children and adolescents and 20 healthy control subjects was compared for a period of approximately 5 years. Fasting blood glucose, fructosamine, and glycosylated hemoglobin (HbA1) values were determined at baseline and 5 years later. The differences in ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1997.68.2.136
更新日期:1997-02-01 00:00:00
abstract:BACKGROUND:Crestal bone thickness is a critical determinant of peri-implant tissue stability. This retrospective observational study sought to quantify the buccal bone thickness achieved adjacent to virtual dental implants following guided bone regeneration (GBR) surgery and evaluate the influence of patient- and proce...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1002/JPER.18-0304
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Tobacco and some of its volatile and non-volatile components have been found to affect many types of cells including gingival fibroblasts. Since normal gingival fibroblast functioning is fundamental to the maintenance of the periodontal connective tissue, as well as to wound healing, we examined the effect o...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2000.71.3.425
更新日期:2000-03-01 00:00:00
abstract:BACKGROUND:With declining general health, the maintenance of oral health becomes increasingly difficult. Unfortunately, health care resources are limited. An assessment tool for detecting a patient's need for oral health care assistance would promote its adequate distribution. The aim of this study is to evaluate if th...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2015.150349
更新日期:2016-01-01 00:00:00
abstract::This article describes the occurrence of numerous calcifications, amyloid deposits, and islands of odontogenic epithelium in the gingiva of 3 siblings with familial gingival fibromatosis. These microscopic features have not been reported previously in patients with this condition. ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1995.66.11.1008
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND:A 43-year-old African American male initially presented for a dental evaluation of a recurrent swelling on the buccal aspect of tooth #12. His medical history was unremarkable except for a 20-pack year history of smoking. He was eventually diagnosed as having a necrotic pulp #12, and received root canal trea...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.090418
更新日期:2010-05-01 00:00:00
abstract:BACKGROUND:Mounting evidence supports a connection between periodontal disease and coronary heart disease, ischemic stroke, and diabetes. This theoretically grounded study examines the knowledge, attitudes, and screening and referral behaviors of primary care nurse practitioners (NPs) in relation to the periodontal dis...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2010.100259
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:The aim of this split-mouth study is to compare long-term (18 to 35 years) periodontal conditions of sites treated with gingival augmentation procedures (GAPs) and untreated homologous contralateral sites. METHODS:Forty-seven patients with 64 sites (test group), with lack of attached gingiva associated with...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2016.160284
更新日期:2016-12-01 00:00:00
abstract::The aims of this paper are to briefly describe laser physics, the types of lasers currently available for use on soft tissues focusing primarily on CO2 and Nd:YAG laser energies, the histological effects of lasers on oral tissues, laser safety, the clinical applications of lasers on oral soft tissues, and future direc...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1993.64.7.589
更新日期:1993-07-01 00:00:00
abstract::A retrospective study of tooth loss in 211 patients who were treated for periodontal disease in private practice and maintained for 15 to 34 years on 3- to 6-month recall schedules is reported. The average age of the patients was 42 years, and the average length of time in maintenance was 22 years. On the basis of res...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1986.57.6.347
更新日期:1986-06-01 00:00:00
abstract:BACKGROUND:Chronic periodontitis (CP) exhibits inflammation and alveolar bone loss, and severe forms of periodontitis are suggested to have a genetic basis. Vitamin D receptor (VDR) regulates bone metabolism and inflammation-related genes, and single nucleotide polymorphisms (SNPs) in the VDR gene may affect the functi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080465
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:Epidemiologic and randomized controlled studies have shown that periodontal diseases may be associated with preterm labor and delivery of infants with low birth weights. The purpose of the present study was to determine the presence of microbial invasion of the amniotic cavity by periodontopathic bacteria in...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2007.060368
更新日期:2007-07-01 00:00:00
abstract:BACKGROUND:Porphyromonas gingivalis is one of the major microbial pathogens associated with chronic periodontitis. To eradicate such pathogens by periodontal therapy, it is essential to clarify the source of infection. Recent findings suggest that the genotype of the fimbriae is one of the important factors in infectio...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2003.74.9.1355
更新日期:2003-09-01 00:00:00
abstract::The interpretation of dental radiographs for the diagnosis of periodontal disease conditions poses several difficulties. These include the inability to adequately reproduce the projection geometry and optical density of the exposures. In order to improve the ability to extract accurate quantitative information from a ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1983.54.7.435
更新日期:1983-07-01 00:00:00
abstract::Primary socket closure in immediate implantation may be difficult, due to the opening left by the extracted tooth. The absolute need for bone augmentation and primary flap closure for implants placed into fresh extraction sites has never been proven. The hypothesis of the present study was that immediate implants can ...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1998.69.7.743
更新日期:1998-07-01 00:00:00
abstract::The purpose of this study was to evaluate the healing potential of deep, primarily 3wall intrabony defects which were treated by flap debridement and expanded polytetrafluoroethylene (ePTFE) membranes and followed for extended time periods. All of the treated defects were in the mandibular arch. Thirty-two patients we...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1993.64.11s.1138
更新日期:1993-11-01 00:00:00
abstract::The inability to examine initiation and progression of periodontal disease and to assess certain therapies in humans has led to a great interest in the use of animal models in periodontal research. Some of the most prominent animals used are non-human primates. This article reviews the characteristics of non-human pri...
journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1993.64.6.497
更新日期:1993-06-01 00:00:00
abstract::It is generally recognized that bacteria in dental plaque at sites of periodontal diseases are not commonly found at sites of periodontal health. One hypothesis to explain the etiology of periodontitis is that pathogenic bacteria from diseased sites infect healthy sites. It has been suggested that gingival inflammatio...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1996.67.2.109
更新日期:1996-02-01 00:00:00
abstract:BACKGROUND:To our knowledge, prospective studies (matched for sex, smoking, and diabetes) that investigated the influence of compliance in the progression of periodontitis and tooth loss in periodontal maintenance therapy (PMT) programs were not previously reported. METHODS:A total of 58 regular complier (RC) and 58 e...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2011.100664
更新日期:2011-09-01 00:00:00
abstract::A histologic review of 129 circumscribed mesenchymal lesions of the gingivae was performed. Two cases were peripheral reparative giant cell granulomas; eight were peripheral odontogenic fibromas; 41 were inflammatory hyperplasias; 68 were pyogenic granulomas; 11 were "hard fibromas"; one lesion exhibited the histologi...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1978.49.8.423
更新日期:1978-08-01 00:00:00
abstract:BACKGROUND:Pain generated by needle sticks (Ns) for the delivery of local anesthetic and/or scaling and root planing (SRP) instrumentation is commonly addressed by the use of topical anesthetics, such as a benzocaine-gel preparation (BGP). Pain suppression following such use has been highly variable. Development of med...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.2001.72.4.479
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND:The use of nanoparticles of graft materials may lead to breakthrough applications for periodontal regeneration. However, due to their small particle size, nanoparticles may be eliminated from periodontal defects by phagocytosis. In an attempt to improve nanoparticle retention in periodontal defects, the pres...
journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2013.120701
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND:Cyclosporin A (CsA) induces gingival overgrowth (GO) in patients who seem to be prone to this disorder. It is still impossible to determine which patients will develop GO. Patients treated with the new immunosuppressive drug tacrolimus seem not to have GO. The aims of this study were to investigate transform...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2006.050086
更新日期:2006-05-01 00:00:00
abstract::This investigation was undertaken to compare the Gindex saliva test scores with the Gingival Index scores and crevicular fluid flow scores, as proposed by Löe and Holm-Pedersen. Eighty-one patients were tested using the Gindex chemical analysis kit. Crevicular fluid was collected from the six teeth advocated by Ramfjo...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1978.49.11.564
更新日期:1978-11-01 00:00:00
abstract::Healing of extraction sockets where the buccal plate of bone has been destroyed often results in localized ridge deformities. This is most likely due to the ingrowth of extraskeletal connective tissue into the space originally occupied by the tooth which then fills with a combination of bone and soft tissue. Technique...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.1.17
更新日期:1994-01-01 00:00:00
abstract::This investigation was conducted on seven individuals referred for treatment of moderate adult periodontitis. Twenty-eight sites with an average probing depth of 5.6 +/- 0.9 mm were evaluated. One tooth per jaw quadrant was randomly assigned to receive one of the following treatments: (I) no treatment, i.e., control; ...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.1988.59.5.301
更新日期:1988-05-01 00:00:00
abstract::The capacity of epithelial cells to migrate and grow from gingival explants cultured on mineralized and partially demineralized root surfaces in an in vitro system was assessed. Explants of attached gingiva were obtained from mongrel dogs, cut into rectangular pieces (1 x 2 mm) and cultured either on mineralized or pa...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1988.59.8.531
更新日期:1988-08-01 00:00:00
abstract::The recognition that periodontal diseases are primarily caused by specific microorganisms has led researchers to explore the possibility that antibiotics may enhance the effect of mechanical debridement procedures such as scaling and surgery. For some selected periodontal diseases, this has proven to be true. This pap...
journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1993.64.8s.760
更新日期:1993-08-01 00:00:00