Volatile components of cigarette smoke: effect of acrolein and acetaldehyde on human gingival fibroblasts in vitro.

Abstract:

BACKGROUND:Tobacco and some of its volatile and non-volatile components have been found to affect many types of cells including gingival fibroblasts. Since normal gingival fibroblast functioning is fundamental to the maintenance of the periodontal connective tissue, as well as to wound healing, we examined the effect of acrolein and acetaldehyde, volatile components of cigarette smoke, on proliferation, attachment, and ultrastructure of human gingival fibroblasts (HGFs) in culture. METHODS:Human gingival fibroblast (HGF) strains derived from healthy individuals with non-inflamed gingiva were used in this study. The cells were incubated in the presence of different concentrations of acrolein and acetaldehyde. Cell attachment and proliferation were evaluated after incubation for 3 hours and 5 days, respectively. In addition, the cells were examined with a transmission electron microscope in order to evaluate their morphology. RESULTS:The results show that acrolein and acetaldehyde produced dose-dependent inhibition of HGF attachment and proliferation. The cytotoxic effect was, however, reversible when both substances were removed, after 3 days, from the medium. The main ultrastructural finding for the HGF cytoplasm was the presence of vacuoles and lysosomal structures that became prominent with increasing concentration of acrolein and acetaldehyde. CONCLUSIONS:Our experimental data suggest that acrolein and acetaldehyde, volatile components of tobacco smoke, are detrimental to HGF survival and consequently to the oral connective tissue. According to our morpho-functional evidence, these findings corroborate clinical and epidemiological investigations demonstrating smoke as a risk factor in the development of periodontal disease.

journal_name

J Periodontol

authors

Cattaneo V,Cetta G,Rota C,Vezzoni F,Rota MT,Gallanti A,Boratto R,Poggi P

doi

10.1902/jop.2000.71.3.425

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

425-32

issue

3

eissn

0022-3492

issn

1943-3670

journal_volume

71

pub_type

杂志文章
  • Effect of citric acid and lyophilized autologous plasma on healing following periodontal flap surgery in monkeys.

    abstract::The purpose of this histologic, histometric, and autoradiographic study was to examine the effect of citric acid conditioning and lyophilized autologous plasma (LAP) application on healing following periodontal flap surgery. Mucoperiosteal flaps were elevated in six rhesus monkeys using the modified Widman flap proced...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1987.58.11.770

    authors: Nasjleti CE,Caffesse RG,Castelli WA,Smith BA,Lopatin DE,Kowalski CJ

    更新日期:1987-11-01 00:00:00

  • Human gingival Langerhans cells stimulate allogeneic lymphocytes: requirement for MHC class II antigens.

    abstract::Langerhans cells (LC) are antigen-presenting cells which express high levels of Class II MHC antigens on their plasma membranes. While the expression of these antigens on gingival LC has been documented, their functional significance is unclear. In this study, the mixed epithelial cell-lymphocyte culture reaction (MEC...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1990.61.6.328

    authors: Walsh LJ,Seymour GJ,Powell RN

    更新日期:1990-06-01 00:00:00

  • The interaction between serum amyloid A and Toll-like receptor 2 pathway regulates inflammatory cytokine secretion in human gingival fibroblasts.

    abstract:BACKGROUND:Serum amyloid A (SAA) has been identified to trigger inflammation response, and play a crucial role in chronic inflammatory diseases. However, the regulatory mechanism of SAA still remains unclear during the development of periodontitis METHODS: SAA mRNA and protein expression were detected in healthy and in...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1002/JPER.19-0050

    authors: Song LT,Lai W,Li JS,Mu YZ,Li CY,Jiang SY

    更新日期:2020-01-01 00:00:00

  • Low-level laser therapy in the treatment of mucous membrane pemphigoid: a promising procedure.

    abstract:BACKGROUND:Mucous membrane pemphigoid is a heterogeneous group of autoimmune, subepithelial, blistering diseases. A combination of topical and systemic steroid treatment is often used when managing patients with mucous membrane pemphigoid. The use of systemic steroids presents an increased risk of adverse side effects....

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2010.100095

    authors: Yilmaz HG,Kusakci-Seker B,Bayindir H,Tözüm TF

    更新日期:2010-08-01 00:00:00

  • Gingival Margin Stability After Mucogingival Plastic Surgery. The Effect of Manual Versus Powered Toothbrushing: A Randomized Clinical Trial.

    abstract:BACKGROUND:Oscillating-rotating power toothbrushes have been proven to be clinically efficacious. To the best of the authors' knowledge, a clinical evaluation of the safety of these toothbrushes after surgical root coverage procedures has not been published. The aim of this study is to evaluate the gingival margin (GM)...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,随机对照试验

    doi:10.1902/jop.2016.150528

    authors: Acunzo R,Limiroli E,Pagni G,Dudaite A,Consonni D,Rasperini G

    更新日期:2016-10-01 00:00:00

  • Coping with stress: its influence on periodontal disease.

    abstract:BACKGROUND:Various forms of stress behavior were documented and in patients with periodontitis their relationship with periodontal disease was investigated. METHODS:Eighty-nine patients with different forms of chronic periodontitis were included in this retrospective case-control study. They were all undergoing period...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2002.73.11.1343

    authors: Wimmer G,Janda M,Wieselmann-Penkner K,Jakse N,Polansky R,Pertl C

    更新日期:2002-11-01 00:00:00

  • The influence of interleukin gene polymorphism on expression of interleukin-1beta and tumor necrosis factor-alpha in periodontal tissue and gingival crevicular fluid.

    abstract:BACKGROUND:A specific composite genotype of the polymorphic interleukin-1 (IL-1) gene cluster has recently been associated with severe periodontitis. One polymorphism of the composite periodontitis-associated genotype (PAG) has been functionally linked with expression of high levels of IL-1. The purpose of this study w...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1999.70.6.567

    authors: Engebretson SP,Lamster IB,Herrera-Abreu M,Celenti RS,Timms JM,Chaudhary AG,di Giovine FS,Kornman KS

    更新日期:1999-06-01 00:00:00

  • Periodontal surgery of vertical bony defects with or without synthetic bioabsorbable barriers. 12-month results.

    abstract::The aim of the present study was to clinically and radiographically compare guided tissue regeneration (GTR) therapy with bioabsorbable polyglactin 910 barriers and conventional periodontal surgery in intrabony defects. In 26 patients with advanced periodontitis, 29 teeth exhibiting interproximal intrabony defects wer...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章

    doi:10.1902/jop.1998.69.11.1210

    authors: Eickholz P,Lenhard M,Benn DK,Staehle HJ

    更新日期:1998-11-01 00:00:00

  • Immunohistochemical expression of extracellular matrix components of normal and healing periodontal tissues in the beagle dog.

    abstract::Periodontal regeneration requires formation of periodontal tissues lost due to periodontal disease. To better understand the formation of new periodontal tissues during periodontal repair and regeneration, immunohistochemical expression of extracellular matrix components of normal as well as healing periodontal tissue...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1995.66.7.579

    authors: Matsuura M,Herr Y,Han KY,Lin WL,Genco RJ,Cho MI

    更新日期:1995-07-01 00:00:00

  • Effects of plaque control on the patency of dentinal tubules: an in vivo study in beagle dogs.

    abstract:BACKGROUND:The aim of this in vivo study was to evaluate the effects of plaque control on the patency of dentinal tubules using vital teeth of beagle dogs. METHODS:Class V cavities were prepared on the cervical areas of the mandibular and maxillary molars in each dog with a diamond point. To simulate the state of dent...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2006.050159

    authors: Suge T,Kawasaki A,Ishikawa K,Matsuo T,Ebisu S

    更新日期:2006-03-01 00:00:00

  • An evaluation of the efficacy of a curved bristle and conventional toothbrush. A comparative clinical study.

    abstract:BACKGROUND:The aim of this study was to determine the plaque-removing ability of a curved bristle toothbrush compared to a conventional, straight bristle, manual toothbrush. METHODS:The study group consisted of 100 volunteers 16 to 24 years of age from a professional engineering college. A four-week post-prophylaxis, ...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1902/jop.2000.71.5.785

    authors: Chava VK

    更新日期:2000-05-01 00:00:00

  • An evaluation of laser Doppler readings obtained from human gingival sulci.

    abstract::A small laser doppler probe with an outside diameter comparable to a conventional periodontal probe (0.5 mm) was developed. The objectives of this investigation were to determine if intrasulcular laser doppler readings (LDR): 1) are reproducible; 2) can detect vascular changes associated with trauma from probe placeme...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章

    doi:10.1902/jop.1995.66.3.171

    authors: Hinrichs JE,LaBelle LL,Aeppli D

    更新日期:1995-03-01 00:00:00

  • A short-term study of the effects of SBHAN, a novel compound, on gingival inflammation in the beagle dog.

    abstract::Unique hydroxyl ion-modulating compounds based on the amino acid glycine have been developed that possess both antimicrobial and pro-healing properties. The purpose of the present study was to determine the effects of one of these compounds, 8.5% (w/v) sodium N, N-bis-2 (hydroxylethyl) aminoacetate (SBHA) with 0.3% (w...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1997.68.5.448

    authors: Cutler CW,Ghaffar KA

    更新日期:1997-05-01 00:00:00

  • Treatment of aggressive periodontitis by osseointegrated dental implants. A case report.

    abstract:BACKGROUND:Generalized aggressive periodontitis is described as a clinical entity affecting both deciduous and permanent dentition with extensive alveolar bone loss, mobility, and exfoliation of all or many teeth. Controversy exists on dental implant use to restore missing dentition in younger patients. METHODS:This c...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2001.72.3.411

    authors: Yalçin S,Yalçin F,Günay Y,Bellaz B,Onal S,Firatli E

    更新日期:2001-03-01 00:00:00

  • The effect of a supragingival plaque-control regimen on the subgingival microbiota in smokers and never-smokers: evaluation by real-time polymerase chain reaction.

    abstract:BACKGROUND:The aim of the present study was to evaluate the effect of strict supragingival plaque control on the subgingival microbiota in smokers and never-smokers. Research into the impact of supragingival plaque control on the number of subgingival bacteria has resulted in contradictory findings. Real-time polymeras...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章

    doi:10.1902/jop.2008.070558

    authors: Gomes SC,Nonnenmacher C,Susin C,Oppermann RV,Mutters R,Marcantonio RA

    更新日期:2008-12-01 00:00:00

  • Biochemical and clinical factors influencing oral malodor in periodontal patients.

    abstract::The amounts of volatile sulfur compounds (VSC) and methyl mercaptan/hydrogen sulfide ratio in mouth air from patients with periodontal involvement were 8 times greater than those of control subjects. Our studies demonstrated that, in patients with periodontal disease: 1) the concentration of disulfide, which is conver...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,评审

    doi:10.1902/jop.1992.63.9.783

    authors: Yaegaki K,Sanada K

    更新日期:1992-09-01 00:00:00

  • The prevention of localized ridge deformities using guided tissue regeneration.

    abstract::Healing of extraction sockets where the buccal plate of bone has been destroyed often results in localized ridge deformities. This is most likely due to the ingrowth of extraskeletal connective tissue into the space originally occupied by the tooth which then fills with a combination of bone and soft tissue. Technique...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1994.65.1.17

    authors: O'Brien TP,Hinrichs JE,Schaffer EM

    更新日期:1994-01-01 00:00:00

  • Association of plasma 25-hydroxyvitamin d concentrations and pathogenic oral bacteria in postmenopausal females.

    abstract:BACKGROUND:Previous findings of an association between 25-hydroxyvitamin D [25(OH)D] concentrations and periodontal disease may be partially explained by the antimicrobial properties of vitamin D. To the best of the authors' knowledge, no study has investigated the association between 25(OH)D and pathogenic oral bacter...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2013.130518

    authors: Sahli MW,Wactawski-Wende J,Ram PK,LaMonte MJ,Hovey KM,Genco RJ,Andrews CA,Millen AE

    更新日期:2014-07-01 00:00:00

  • Salivary and plasma levels of Toll-like receptor 2 and Toll-like receptor 4 in chronic periodontitis.

    abstract:BACKGROUND:This cross-sectional study was planned to investigate whether patients with chronic periodontitis exhibit different salivary or plasma concentrations of Toll-like receptor (TLR) 2 and TLR4 compared to subjects who are clinically healthy. METHODS:Whole saliva and plasma samples were obtained and full-mouth c...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2010.100467

    authors: Buduneli N,Özçaka Ö,Nalbantsoy A

    更新日期:2011-06-01 00:00:00

  • A laboratory model biofilm fermenter: design and initial trial on a single species biofilm.

    abstract:BACKGROUND:The minimum inhibitory concentration (MIC) does not provide information on the efficacy of antimicrobial agents against infections involving biofilms, which are many times more resistant than planktonic forms of bacteria. This report is on the design and initial trial of a device for growing standard biofilm...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2005.76.9.1443

    authors: Wirthlin MR,Chen PK,Hoover CI

    更新日期:2005-09-01 00:00:00

  • Factitious gingival ulceration: as presenting sign of Munchausen's syndrome?

    abstract::This report describes the case of a 21-year-old female who presented with localized gingival ulceration that over a period of 4 weeks became generalized, affecting all the buccal gingiva as well as the lower lip. A detailed medical history suggested that the patient was generating the lesions herself. She was monitore...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1994.65.5.442

    authors: Heasman PA,MacLeod I,Smith DG

    更新日期:1994-05-01 00:00:00

  • Periodontal wound healing with and without platelet-rich plasma: histologic observations and assessment of flap tensile strength.

    abstract:BACKGROUND:Platelet-rich plasma (PRP) has been promoted as a surgical adjunct to enhance hard and soft tissue wound healing. Although anecdotally reported to be of value, the results of controlled studies examining the added effects of PRP on surgical procedures have been mixed. The purpose of this study was to test th...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2009.080626

    authors: Powell CA,Bannister SR,Mackey SA,Maller SC,McDonnell HT,Deas DE

    更新日期:2009-06-01 00:00:00

  • Evaluation of the titanium Ti-6Al-7Nb alloy with and without plasma-sprayed hydroxyapatite coating on growth and viability of cultured osteoblast-like cells.

    abstract:BACKGROUND:Osseointegrated dental implants are currently recognized as a standard treatment method in dentistry. Titanium (Ti) and its alloys are the metals of choice for endosseous parts of currently available dental implants. Ti-6Al-4V is the most used Ti alloy, however; an improved version, Ti-6Al-7Nb, has been rece...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2002.73.8.900

    authors: de Lavos-Valereto IC,Deboni MC,Azambuja N Jr,Marques MM

    更新日期:2002-08-01 00:00:00

  • Local oxygen therapy for treating acute necrotizing periodontal disease in smokers.

    abstract:BACKGROUND:The main aim of treatment for acute necrotizing periodontal disease is fast and effective reduction of anaerobic destructive microorganisms to avoid periodontal damage. The effect of adjunctive local oxygen therapy in the treatment of necrotizing periodontal disease was examined in this study. METHODS:Thirt...

    journal_title:Journal of periodontology

    pub_type: 杂志文章,随机对照试验

    doi:10.1902/jop.2006.77.1.31

    authors: Gaggl AJ,Rainer H,Grund E,Chiari FM

    更新日期:2006-01-01 00:00:00

  • Langerhans-cell histiocytosis: a clinical case without bone involvement.

    abstract:BACKGROUND:Langerhans-cell histiocytosis (LCH) is a group of rare disorders histologically characterized by the proliferation of Langerhans cells. Multiple organs and systems may be involved by the disease. Typically, there is bone involvement and, less frequently, lesions may be found in the lungs, liver, lymph nodes,...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2005.76.1.143

    authors: Manfredi M,Corradi D,Vescovi P

    更新日期:2005-01-01 00:00:00

  • Demineralization of the contacting surfaces in autologous onlay bone grafts improves bone formation and bone consolidation.

    abstract:BACKGROUND:Autologous bone grafts are usually well consolidated after 4 to 5 months but can be incompletely interlocked with the native bone. This study investigated the effect of acid demineralization of the graft-bed interface on graft consolidation. METHODS:Onlay bone grafts were performed on the calvaria of 36 gui...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2013.130298

    authors: Rezende ML,Consolaro A,Sant'Ana AC,Damante CA,Greghi SL,Passanezi E

    更新日期:2014-05-01 00:00:00

  • Absence of histologic signs of chronic inflammation following closed subgingival scaling and root planing using the dental endoscope: human biopsies - a pilot study.

    abstract:BACKGROUND:The primary goal of periodontal therapy is the reduction or elimination of inflammation. Traditionally, this is accomplished through removal of subgingival tooth-borne accretions using non-surgical and/or surgical treatment modalities. Numerous studies indicated the difficulty in removing these accretions to...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2008.080190

    authors: Wilson TG Jr,Carnio J,Schenk R,Myers G

    更新日期:2008-11-01 00:00:00

  • Preprosthetic periodontal surgery in the interproximal area with modification of the COL area: anatomic and histologic study in dogs.

    abstract:BACKGROUND:Due to its concave morphology, a COL creates difficulties for proper oral hygiene. When this characteristic is accentuated by tooth position or caries and when restorations are necessary, they should be corrected by preprosthetic surgery. However, there are no data proving the efficacy of the procedures. The...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.2001.72.12.1734

    authors: Novaes AB Jr,Novaes AB,De Oliveira PT

    更新日期:2001-12-01 00:00:00

  • Day-long reduction of oral malodor by a two-phase oil:water mouthrinse as compared to chlorhexidine and placebo rinses.

    abstract::Few scientific investigations have addressed the ability of mouthrinses to reduce oral malodor for periods longer than 3 hours. In the present report, we have employed simple, recently described techniques to assess the day-long reduction in oral malodor of a novel 2-phase oil:water mouthrinse (TPM), as compared to a ...

    journal_title:Journal of periodontology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1902/jop.1992.63.1.39

    authors: Rosenberg M,Gelernter I,Barki M,Bar-Ness R

    更新日期:1992-01-01 00:00:00

  • Root isolation for new attachment procedures. A surgical and suturing method: three case reports.

    abstract::A surgical and suturing method is described for the subgingival placement of Gore-tex periodontal material during new attachment procedures. The periodontal material isolates the root surface from epithelium and gingival connective tissue. Three cases were treated. Clinical new attachment was evident from clinical pro...

    journal_title:Journal of periodontology

    pub_type: 杂志文章

    doi:10.1902/jop.1987.58.12.819

    authors: Becker W,Becker BE,Prichard JF,Caffesse R,Rosenberg E,Gian-Grasso J

    更新日期:1987-12-01 00:00:00