Abstract:
BACKGROUND:Tobacco and some of its volatile and non-volatile components have been found to affect many types of cells including gingival fibroblasts. Since normal gingival fibroblast functioning is fundamental to the maintenance of the periodontal connective tissue, as well as to wound healing, we examined the effect of acrolein and acetaldehyde, volatile components of cigarette smoke, on proliferation, attachment, and ultrastructure of human gingival fibroblasts (HGFs) in culture. METHODS:Human gingival fibroblast (HGF) strains derived from healthy individuals with non-inflamed gingiva were used in this study. The cells were incubated in the presence of different concentrations of acrolein and acetaldehyde. Cell attachment and proliferation were evaluated after incubation for 3 hours and 5 days, respectively. In addition, the cells were examined with a transmission electron microscope in order to evaluate their morphology. RESULTS:The results show that acrolein and acetaldehyde produced dose-dependent inhibition of HGF attachment and proliferation. The cytotoxic effect was, however, reversible when both substances were removed, after 3 days, from the medium. The main ultrastructural finding for the HGF cytoplasm was the presence of vacuoles and lysosomal structures that became prominent with increasing concentration of acrolein and acetaldehyde. CONCLUSIONS:Our experimental data suggest that acrolein and acetaldehyde, volatile components of tobacco smoke, are detrimental to HGF survival and consequently to the oral connective tissue. According to our morpho-functional evidence, these findings corroborate clinical and epidemiological investigations demonstrating smoke as a risk factor in the development of periodontal disease.
journal_name
J Periodontoljournal_title
Journal of periodontologyauthors
Cattaneo V,Cetta G,Rota C,Vezzoni F,Rota MT,Gallanti A,Boratto R,Poggi Pdoi
10.1902/jop.2000.71.3.425subject
Has Abstractpub_date
2000-03-01 00:00:00pages
425-32issue
3eissn
0022-3492issn
1943-3670journal_volume
71pub_type
杂志文章abstract::The purpose of this histologic, histometric, and autoradiographic study was to examine the effect of citric acid conditioning and lyophilized autologous plasma (LAP) application on healing following periodontal flap surgery. Mucoperiosteal flaps were elevated in six rhesus monkeys using the modified Widman flap proced...
journal_title:Journal of periodontology
pub_type: 杂志文章
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更新日期:1987-11-01 00:00:00
abstract::Langerhans cells (LC) are antigen-presenting cells which express high levels of Class II MHC antigens on their plasma membranes. While the expression of these antigens on gingival LC has been documented, their functional significance is unclear. In this study, the mixed epithelial cell-lymphocyte culture reaction (MEC...
journal_title:Journal of periodontology
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journal_title:Journal of periodontology
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更新日期:2016-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of periodontology
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
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更新日期:1998-11-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2000-05-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
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更新日期:1995-03-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
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更新日期:1997-05-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2001.72.3.411
更新日期:2001-03-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章
doi:10.1902/jop.2008.070558
更新日期:2008-12-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章,评审
doi:10.1902/jop.1992.63.9.783
更新日期:1992-09-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1994.65.1.17
更新日期:1994-01-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2013.130518
更新日期:2014-07-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
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更新日期:2011-06-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.9.1443
更新日期:2005-09-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2009.080626
更新日期:2009-06-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2002.73.8.900
更新日期:2002-08-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章,随机对照试验
doi:10.1902/jop.2006.77.1.31
更新日期:2006-01-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2005.76.1.143
更新日期:2005-01-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2013.130298
更新日期:2014-05-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2008.080190
更新日期:2008-11-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.2001.72.12.1734
更新日期:2001-12-01 00:00:00
abstract::Few scientific investigations have addressed the ability of mouthrinses to reduce oral malodor for periods longer than 3 hours. In the present report, we have employed simple, recently described techniques to assess the day-long reduction in oral malodor of a novel 2-phase oil:water mouthrinse (TPM), as compared to a ...
journal_title:Journal of periodontology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1902/jop.1992.63.1.39
更新日期:1992-01-01 00:00:00
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journal_title:Journal of periodontology
pub_type: 杂志文章
doi:10.1902/jop.1987.58.12.819
更新日期:1987-12-01 00:00:00