Preoperative assessment of tumor angiogenesis by vascular endothelial growth factor mRNA expression in homogenate samples of breast carcinoma: fine-needle aspirates vs. resection samples.

Abstract:

BACKGROUND AND OBJECTIVES:Tumor angiogenesis is receiving increased attention as a prognostic factor and also as a possible target for new anticancer agents. We investigated whether extent of vascular endothelial growth factor (VEGF) mRNA expression correlated with degree of neovascularization, and whether this expression in fine-needle aspirates could be a marker for assessing angiogenic potential of breast tumors. METHODS:VEGF mRNA expression was semiquantitated by reverse transcriptase-polymerase chain reaction (RT-PCR) followed by Southern blotting. Tumor neovascularization was assessed by immunohistochemical staining with anti-CD31 (PECAM) antibody. RESULTS:There was a positive correlation between degree of neovascularization and semiquantitated VEGF mRNA expression in invasive ductal carcinomas (r2 = 0.346, n = 48, P < 0.05). Extent of VEGF mRNA expression in fine-needle aspirates was closely correlated with that in resected invasive ductal carcinomas equal to or less than 3 cm in size (r2 = 0.874, n = 14, P < 0.05). CONCLUSION:These data suggest that semiquantitation of VEGF mRNA expression in fine-needle aspirates is useful for assessing angiogenic potential of invasive ductal carcinomas.

journal_name

J Surg Oncol

authors

Anan K,Morisaki T,Katano M,Ikubo A,Tsukahara Y,Uchiyama A,Kuroki S,Tanaka M,Torisu M

doi

10.1002/(sici)1096-9098(199712)66:4<257::aid-jso7>

subject

Has Abstract

pub_date

1997-12-01 00:00:00

pages

257-63

issue

4

eissn

0022-4790

issn

1096-9098

pii

10.1002/(SICI)1096-9098(199712)66:4<257::AID-JSO7>

journal_volume

66

pub_type

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