Abstract：
:The binding of the lectin Concanavalin-A (Con-A) to crystallins was investigated. For this purpose, human fetal and juvenile lens crystallins were isofocused and specifically stained brown for glycoproteins by the lectin Con-A, and purple by the periodic acid Schiff's reagent (PAS). In reference experiments protein bands were stained with Coomassie Blue for proteins. Since Con-A is a protein with glucose-specific receptors, the following crystallins, glycated with this sugar, were visualized after isoelectric focusing: HM-, beta L-, beta S- and gamma-crystallins, but not alpha L- and beta H-crystallins. Glycation increased significantly with fetal age. The crystallins themselves could also function as sugar receptors, because it was shown that they possessed also receptors for glucose, like Con-A. This crystallin receptor staining revealed beta L-, beta S-, gamma- but hardly HM-crystallins. The PAS, Con-A and receptor stainings gave in principle identical results. The glycoproteins Con-A, horseradish peroxidase and lentil lectins were used as positive controls.
journal_name
Mech Ageing Devjournal_title
Mechanisms of ageing and developmentauthors
Ahrend MH,Bours Jdoi
10.1016/s0047-6374(97)00097-3subject
Has Abstractpub_date
1997-12-30 00:00:00pages
167-79issue
3eissn
0047-6374issn
1872-6216pii
S0047637497000973journal_volume
99pub_type
杂志文章abstract：:The growing proportion of older people, especially the very old, is often thought of in negative terms such as the need for more health care. But the implications are wider and affect the future of family care as well as issues of intergenerational relations, employment, living arrangements and attitudes. This paper l...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/s0047-6374(01)00418-3
更新日期：2002-04-01 00:00:00
abstract：:Lipofuscin-specific autofluorescence of living, cultured rat neonatal myocardial myocytes was studied with respect to intensity and spectral characteristics. The autofluorescence emission spectrum changed over time indicating continuously ongoing intramolecular reorganisation of the pigment. Moreover, as compared with...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(94)90040-x
更新日期：1994-01-01 00:00:00
abstract：:The various phases of homo- and heteroclonal encounters between stolons of Podocoryne carnea M. Sars have been studied with the light and electron microscope. The first clone-unspecific actions and reactions to an imminent encounter between the outgrowing tip of stolon and the subterminal part of an opposed homologous...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(83)90045-3
更新日期：1983-03-01 00:00:00
abstract：:A morphometric investigation has been carried out on the synaptic junctions in the cerebellar glomeruli of young-adult rats chronically deprived of vitamin E for 10 months and control animals of the same age. The following parameters were evaluated: the average length of the synapses (L), the numerical (NV) as well as...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(84)90073-3
更新日期：1984-02-01 00:00:00
abstract：:To test the hypothesis that the aging mammalian heart and brain might have increased vulnerability to acute stress, DNA fragmentation was studied after hypoxia-reoygenation in young adult (6 months) and old (22-24 months) F344 rats. Heart and brain tissue were examined at the following time points: 30, 60, or 90 min o...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(99)00048-2
更新日期：1999-12-07 00:00:00
abstract：:Mutated huntingtin is expressed in nervous and non nervous system included lymphoblasts. Eneregetic metabolism is impaired in Huntington's disease (HD) and other neurodegenerative diseases. Human HD lymphoblasts have provided clear-cut data on mitochondnal disruption. Here we report morphological, morphometric and mem...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2005.09.010
更新日期：2006-02-01 00:00:00
abstract：:The circulating antibody titers to bovine serum albumin (BSA) were determined for two groups of aged Fischer 344 rats obtained form the National Institute on Aging. One group received a single immunization of BSA in complete Freund's adjuvant, and the second group was immunized three times at two-week intervals with t...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(81)90032-4
更新日期：1981-05-01 00:00:00
abstract：:BCL-2 functions as a death repressor molecule in an evolutionary conserved cell death pathway. Inactivation of bcl-2 in mice results in pleiotropic effects including postnatal growth retardation, massive apoptosis in lymphoid tissues, polycystic kidney disease (PKD) and shortened lifespan. To evaluate the influence of...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2006.02.009
更新日期：2006-07-01 00:00:00
abstract：:The present work explores the application of information theoretical measures, such as entropy and normalized mutual information, for research of biomarkers of aging. The use of information theory affords unique methodological advantages for the study of aging processes, as it allows evaluating non-linear relations be...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2017.01.003
更新日期：2017-04-01 00:00:00
abstract：:Quantitative measurements of glycogen in aging bruchids showed there was a considerable decrease (> 50%) with age except on the fifth day when an increase was observed, followed by a decrease on the sixth day. The variations in glycogen content with age suggests that glycogen serves as one of the energy sources respon...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(80)90082-2
更新日期：1980-01-01 00:00:00
abstract：:Human diploid cells, TIG-7, were serially cultivated under 1%, 5% or 21% (air) oxygen. The limit of their cumulative population doublings was extended by 16% or 10% under 1% or 5% oxygen, respectively, as compared with that under 21% oxygen. When TIG-7 cells were serially cultivated in the presence of 10 mM or 20 mM 3...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(95)01584-m
更新日期：1995-07-14 00:00:00
abstract：:Although myocardial actomyosin ATPase specific activity determined at Ca2+ levels of 1 x 10(-3) to 1 x 10(-2) M is consistently higher in two-month old male Fischer rats than in twelve-month old individuals, this difference can be eliminated if the enzyme is assayed at lower calcium concentrations. This suggests that ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(78)90040-4
更新日期：1978-12-01 00:00:00
abstract：:The general objective of this study was to examine the relationship between oxygen free radicals and the aging process. The rate of superoxide anion radical (O2.-) generation was measured in liver sub-mitochondrial particles from mouse, rat, rabbit, pig and cow, and in flight muscle sub-mitochondrial particles from th...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/0047-6374(89)90096-1
更新日期：1989-08-01 00:00:00
abstract：:Loss of telomere length homeostasis has been linked to age-related disease especially cancer. In this review, we discuss two major causes of telomere dysfunction that potentially lead to tumorigenesis: replicative aging and environmental assaults. Aging has long been recognized as a source for telomere dysfunction thr...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2007.10.010
更新日期：2008-01-01 00:00:00
abstract：:The purpose of this research is to determine possible causes and mechanisms involved in the age-associated decline in mitochondrial activity. We have focused on cytochrome c oxidase because it is comprised of both nuclear and mitochondrial-encoded subunits and may provide some insight into the coordination of the two ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(98)00043-8
更新日期：1998-06-15 00:00:00
abstract：:Vascular tree development depends on the timely differentiation of endothelial and vascular smooth muscle cells. These latter are key players in the formation of the vascular scaffold that offers resistance to the blood flow. This review aims at providing an overview on the role of PDE5, the cGMP-specific phosphodiest...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2020.111311
更新日期：2020-09-01 00:00:00
abstract：:Active oxygen species have been considered to be responsible for the aging process and for the induction and initiation of neoplastic processes. The effect of hydrogen peroxide, an active oxygen species, was investigated in the chromosomes of three young women (20-21 years of age) and of three elderly women (73-79 yea...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(90)90002-w
更新日期：1990-11-01 00:00:00
abstract：:Previous studies from this laboratory have documented a progressive age-related decline in trophic hormone (or second messenger cAMP) stimulated corticosterone production in isolated adrenocortical cells. In the current study, we examined the possibility that the aging process exerts this effect by interfering with an...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(94)90043-4
更新日期：1994-11-25 00:00:00
abstract：:Elastase and lipid levels of the plasmas of subjects and of those who have suffered a stroke have been measured, and it has been shown that the normal age related fall in elastase content is mirrored more closely by the fall in high density lipoprotein cholesterol than by changes in total cholesterol. After a stroke a...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(80)90003-2
更新日期：1980-11-01 00:00:00
abstract：:Falls following a meal occur commonly in older persons. These falls have been related to a decrease in postprandial blood pressure due to carbohydrates in the meal. The mechanism by which this occurs is not known. In this study, we examined the possible role of a vasodilatory peptide, calcitonin gene-related peptide (...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(96)01688-0
更新日期：1996-06-07 00:00:00
abstract：:The B cell responsiveness mediated by T cells has been evaluated in 50 aged donors by using a Pokeweed mitogen (PWM)-driven differentiation system. Peripheral blood mononuclear cells (PBMC) from aged donors exhibited a lower plaque-forming cell (PFC) generation in comparison with young donors. Similar results were obt...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(87)90056-x
更新日期：1987-11-01 00:00:00
abstract：:Type I and II macrophage scavenger receptors are implicated in the pathologic deposition of cholesterol during the atherogenesis. There is a charged collagen structure of type I and II receptors identified as a ligand binding domain, which can recognize a wide range of negatively charged macromolecules including oxidi...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/s0047-6374(99)00079-2
更新日期：1999-11-01 00:00:00
abstract：:Age-related changes in regulation of receptor response have been observed in several tissues and include regulation of beta-adrenergic receptor (beta-receptor) responses. The role of cellular aging in age-related changes in receptor response is not clear. We have examined the effect of aging in vitro on human fibrobla...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/0047-6374(92)90003-v
更新日期：1992-05-01 00:00:00
abstract：:Aging is characterized by a progressive loss of several physiological functions that can cause various age-related disorders. Several factors have been identified as causes of aging to elucidate the decline in functions. Various aspects of physiological deterioration are controlled by the hypothalamus, a critical brai...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2018.04.008
更新日期：2019-01-01 00:00:00
abstract：:Understanding mechanisms underlying longevity, and endeavor towards the specific goals of alleviating frailty in old age, require a comprehensive approach that considers the various theoretical and experimental approaches, as well as compiling the data on humans. This logistic has underlined the program of the confere...
journal_title：Mechanisms of ageing and development
pub_type： 社论,评审
doi：10.1016/j.mad.2004.08.013
更新日期：2005-02-01 00:00:00
abstract：:Multimorbidity and depression are complex multifactorial conditions with major implications for older individuals, their families, and healthcare providers. In this scoping review, we aimed to 1) review findings from longitudinal epidemiological studies investigating the association between multimorbidity and depressi...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2020.111383
更新日期：2020-12-01 00:00:00
abstract：:Age is the only one non-modifiable risk of cerebral ischemia. Advances in stroke medicine and behavioral adaptation to stroke risk factors and comorbidities was successful in decreasing stroke incidence and increasing the number of stroke survivors in western societies. Comorbidities aggravates the outcome after cereb...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2020.111312
更新日期：2020-09-01 00:00:00
abstract：:The incidence of chronic diseases such as cardiovascular diseases is lower in Mediterranean Southern Europe than Northern Europe. This may be due to a lower level of oxidative stress and a higher antioxidant status in people living around the Mediterranean Sea. Oxidative stress may influence the rate of shortening of ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/j.mad.2012.04.003
更新日期：2012-06-01 00:00:00
abstract：:In a longitudinal design in which each animal was examined repeatedly throughout its life, we used flow cytometry to determine the numbers or proportions of a variety of T lymphocyte subsets in the peripheral blood of mice aged 8-20 months. Half of the 128 mice were given a low calorie diet which is known to extend li...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章
doi：10.1016/s0047-6374(97)01893-9
更新日期：1997-06-01 00:00:00
abstract：:Accumulation of oxidative DNA damage in the human brain has been implicated in etiologies of post-traumatic and age-associated declines in neuronal function. In neurons, because of high metabolic rates and prolonged life span, exposure to free radicals is intense and risk for accumulation of damaged DNA is amplified. ...
journal_title：Mechanisms of ageing and development
pub_type： 杂志文章,评审
doi：10.1016/j.mad.2008.02.001
更新日期：2008-07-01 00:00:00
