Abstract:
:The metabolism of [3H]benzo[a]pyrene (BP) and (-)-trans-[14C]7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene (BP-7,8-diol) was studied in freshly isolated hepatocytes of the wild benthic fish, brown bullhead (Ictalurus nebulosus). Bullhead hepatocytes incubated with 40 microM [3H]BP for 1 h metabolized BP to water soluble metabolites which were separated on silica gel t.l.c. plates to reveal conjugates with glucuronic acid, glutathione, and sulfate (51%, 14% and 4% of total metabolites, respectively). Additional metabolites that were extractable with ethyl acetate were separated by reversed phase HPLC to reveal only two major metabolites: BP-9,10-dihydrodiol and BP-7,8-diol (13% and 2.6% of total metabolites, respectively). Hepatocytes isolated from individual fish displayed an 11-fold variability in the rates at which they metabolized BP (756 +/- 167 pmol x mg dry wt-1 x h-1), which correlated negatively (r = -0.7, P less than 0.01) with an 18-fold variability in the glycogen content of the cells. Hepatocytes isolated from the same fish, in parallel incubations under the same optimum conditions, metabolized BP-7,8-diol 4.5-fold faster than they metabolized BP. The variability in the rate of BP-7,8-diol metabolism was about 7-fold. Major metabolites included glutathione conjugates, glucuronides and sulfates (35%, 25% and 30% of total metabolites, respectively). These conjugates, like those formed from BP, were degradable with gamma-glutamyltransferase, beta-glucuronidase and arylsulfatase, respectively. Ethyl acetate extractable metabolites were predominantly isomeric benzo-ring tetrahydrotetrols (9% of total metabolites). In summary, this study indicates that during short-term incubations bull-head hepatocytes metabolize BP and BP-7,8-diol primarily to conjugated derivatives. The usefulness of thin-layer chromatography for the convenient determination of the rate of BP-7,8-diol metabolism is demonstrated.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Steward AR,Zaleski J,Sikka HCdoi
10.1016/0009-2797(90)90063-ssubject
Has Abstractpub_date
1990-01-01 00:00:00pages
119-38issue
1-2eissn
0009-2797issn
1872-7786pii
0009-2797(90)90063-Sjournal_volume
74pub_type
杂志文章abstract::American oysters (Crassostrea virginica) were exposed to 0.1 ppm cadmium for 0--15 days in a flowing seawater system and then placed into clean flowing seawater for 24 h prior to sacrifice. Whole oysters were homogenized and a cadmium-binding protein isolated and purified by a process of centrifugation, heat-treatment...
journal_title:Chemico-biological interactions
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更新日期:1979-05-01 00:00:00
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journal_title:Chemico-biological interactions
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2017-10-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2005.06.006
更新日期:2005-09-10 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1997-05-02 00:00:00
abstract::S1 nuclease hydrolysis and benzoylated naphthoylated DEAE cellulose (BND-cellulose) chromatography have been used to study the effect of riboflavin and visible light on DNA. Native calf thymus DNA was incubated with riboflavin in the presence of fluorescent light for various time periods and subjected to S1 nuclease h...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(84)90146-7
更新日期:1984-03-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1980-01-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2016-01-25 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章,评审
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更新日期:2010-11-05 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(91)90031-2
更新日期:1991-01-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(89)90019-7
更新日期:1989-01-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(92)90063-q
更新日期:1992-12-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章,评审
doi:10.1016/s0009-2797(00)00198-8
更新日期:2000-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-2797(01)00222-8
更新日期:2001-06-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/s0009-2797(85)80128-9
更新日期:1985-10-01 00:00:00
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journal_title:Chemico-biological interactions
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更新日期:2020-01-25 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1987-01-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1992-01-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1978-02-01 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2002-01-22 00:00:00
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journal_title:Chemico-biological interactions
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
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journal_title:Chemico-biological interactions
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2014-09-05 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:2000-01-15 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/j.cbi.2008.10.007
更新日期:2009-01-15 00:00:00
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journal_title:Chemico-biological interactions
pub_type: 杂志文章
doi:10.1016/0009-2797(79)90089-9
更新日期:1979-06-01 00:00:00
abstract::Chronic exposure of humans to benzene causes severe bone marrow cell depression leading to aplastic anemia. Marrow stromal macrophage dysfunction and deficient interleukin-1 production has been reported for patients with severe aplastic anemia. The stromal macrophage, a target of benzene toxicity, is involved in hemat...
journal_title:Chemico-biological interactions
pub_type: 杂志文章
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更新日期:1995-12-22 00:00:00