Abstract：
:The reaction characteristics of chlorothalonil with glyceraldehyde-3-phosphate dehydrogenase (GPDH), from yeast, (EC 1.2.1.12) were studied in vitro. Enzyme inhibition was related to the amount of [14C]chlorothalonil bound to the protein. Kinetics of enzyme inhibition was non-competitive for the substrate glyceraldehyde-3-phosphate (GAP) (Ki = 0.42 muM). Reversal of enzyme inhibition could not be demonstrated with the low molecular thiol dithiothreitol (DDT), although the thiol did protect the protein against the toxic action of the fungicide. Because 5,5' dithiobis-(2-nitrobenzoic) acid (DTNB) reduced the binding of 14C-labeled fungicide by approximately 90% it is postulated that chlorothalonil affects catalytic activity by reacting with the 4 sulfhydryl sites (cysteine-149) responsible for the binding of GAP. Certain reaction characteristics of the trichloromethyl fulfenyl fungicides with GPDH were found to be similar to those of chlorothalonil. However, chlorothalonil differed from those fungicides in that it did not react with non-thiol groups of either GPDH or alpha-chymotrypsin (alphaCT) and had a slower reaction rate with the GPDH. It is suggested that the differences in reaction rates of the fungicides are due to the molecular size and the chemical nature of the reactive toxiphores.
journal_name
Chem Biol Interactjournal_title
Chemico-biological interactionsauthors
Long JW,Siegel MRdoi
10.1016/0009-2797(75)90069-1subject
Has Abstractpub_date
1975-06-01 00:00:00pages
383-94issue
6eissn
0009-2797issn
1872-7786pii
0009-2797(75)90069-1journal_volume
10pub_type
杂志文章abstract：:Endometriosis is a frequent benign gynecological disease characterized by endometrial tissue outside the uterine cavity. The estimated prevalence in the general population is 6-10%, but this reaches 30-50% in women with infertility and/or pain. As ectopic tissue within the pelvic cavity provokes inflammation, endometr...
journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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更新日期：1980-06-01 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2010.02.015
更新日期：2010-09-06 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2010.04.003
更新日期：2010-06-07 00:00:00
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2009.12.025
更新日期：2010-03-19 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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journal_title：Chemico-biological interactions
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doi：10.1016/j.cbi.2016.02.003
更新日期：2016-03-25 00:00:00
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更新日期：2020-01-05 00:00:00
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journal_title：Chemico-biological interactions
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：2014-10-05 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：1978-09-01 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2007.05.006
更新日期：2007-08-15 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：2000-01-15 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：2017-10-01 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：1982-11-01 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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更新日期：2016-02-05 00:00:00
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
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journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/0009-2797(93)90112-c
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:Cell death induced by intracellular glutathione depletion has been reported to be dependent on the presence of trace amounts of extracellular copper ions. Since little is known about the relationship between glutathione depletion and copper homeostasis, we have in the present study further investigated the r...
journal_title：Chemico-biological interactions
pub_type： 杂志文章
doi：10.1016/j.cbi.2007.01.005
更新日期：2007-04-05 00:00:00
