Microsatellite markers developed for Corallodiscus lanuginosus (Gesneriaceae) and their cross-species transferability.

Abstract:

UNLABELLED: PREMISE OF THE STUDY:Corallodiscus is widespread across China and in adjacent countries to the west and south, with C. lanuginosus covering the entire range of the genus. Microsatellite markers will be useful to address within-complex taxonomic and biogeographic structures of this species. • METHODS AND RESULTS:Fourteen markers were developed using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol. Polymorphism was assessed in 12 individuals each from two populations from West Yunnan, China. Nine primers displayed polymorphisms. The number of alleles per locus ranged from one to six (mean: 2.7). The observed and expected heterozygosities ranged from 0 to 1 and from 0 to 0.772 (average: 0.487 and 0.448). Only one of the polymorphic loci deviated from Hardy-Weinberg equilibrium. Among the four congeneric species, cross-amplification success ranged from two to eight markers. • CONCLUSIONS:The markers will be useful for population genetic and evolutionary history studies for C. lanuginosus and the allied species C. bhutanicus, C. cooperi, and C. kingianus.

journal_name

Am J Bot

authors

Gao LM,Zhang ZR,Zhou P,Möller M,Li DZ

doi

10.3732/ajb.1200178

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

e490-2

issue

12

eissn

0002-9122

issn

1537-2197

pii

ajb.1200178

journal_volume

99

pub_type

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