Characterization of 21 microsatellite markers from cogongrass, Imperata cylindrica (Poaceae), a weed species distributed worldwide.

Abstract:

PREMISE OF THE STUDY:Microsatellite loci were developed from Imperata cylindrica, a traditional medicinal herb in Asia and among the top 10 worst invasive weeds in the world, to aid in the identification of the limits of asexual clonal individuals. METHODS AND RESULTS:A total of 21 microsatellite markers, including 18 polymorphic and three monomorphic loci, were developed from I. cylindrica using a magnetic bead enrichment protocol. The primers amplified dinucleotide, trinucleotide, and complex repeats. The number of alleles ranged from one to 19 per locus, with an observed heterozygosity ranging from 0.09 to 1.00. Several loci deviated significantly from the within-population Hardy-Weinberg equilibrium as a result of asexual clonal reproduction. CONCLUSIONS:These polymorphic markers should be useful tools in further studies on the identification of the range of clonal reproduction units and the selection and classification of the medicinal cultivar.

journal_name

Am J Bot

authors

Chiang YC,Tsai CC,Hsu TW,Chou CH

doi

10.3732/ajb.1200152

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

e428-30

issue

11

eissn

0002-9122

issn

1537-2197

pii

ajb.1200152

journal_volume

99

pub_type

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