Microsatellite markers of an important medicinal plant, Eurycoma longifolia (Simaroubaceae), for DNA profiling.

Abstract:

PREMISE OF THE STUDY:Microsatellite markers of an important medicinal plant, Eurycoma longifolia (Simaroubaceae), were developed for DNA profiling and genetic diversity studies. METHODS AND RESULTS:Eighteen polymorphic microsatellite loci were developed for E. longifolia. The primers were designed from a genomic library enriched for dinucleotide (CT) repeats and screened on 32 samples from a natural population. The number of alleles detected per locus ranged from four to 16, while the observed heterozygosity ranged from 0.097 to 0.938. No significant deviation from Hardy-Weinberg equilibrium was detected in all the 18 loci, and no linkage disequilibrium was found between these loci after conservative Bonferroni correction. CONCLUSIONS:The 18 microsatellite markers of E. longifolia are highly polymorphic and informative. These markers would serve as an important tool for DNA profiling and genetic diversity studies.

journal_name

Am J Bot

authors

Tnah LH,Lee CT,Lee SL,Ng KK,Ng CH,Hwang SS

doi

10.3732/ajb.1000469

subject

Has Abstract

pub_date

2011-05-01 00:00:00

pages

e130-2

issue

5

eissn

0002-9122

issn

1537-2197

pii

ajb.1000469

journal_volume

98

pub_type

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