Optimized genotyping with microsatellite markers in the fungal banana pathogen Mycosphaerella fijiensis (Mycosphaerellaceae).

Abstract:

UNLABELLED: PREMISE OF THE STUDY:Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis, a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. • METHODS AND RESULTS:Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. • CONCLUSIONS:The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations.

journal_name

Am J Bot

authors

Robert S,Rieux A,Argout X,Carlier J,Zapater MF

doi

10.3732/ajb.1000295

subject

Has Abstract

pub_date

2010-12-01 00:00:00

pages

e130-2

issue

12

eissn

0002-9122

issn

1537-2197

pii

ajb.1000295

journal_volume

97

pub_type

杂志文章
  • Seasonal changes in physiological performance in wild Clarkia xantiana populations: Implications for the evolution of a compressed life cycle and self-fertilization.

    abstract:PREMISE OF THE STUDY:One explanation for the evolution of selfing, the drought escape hypothesis, proposes that self-fertilization may evolve under conditions of intensifying seasonal drought as part of a suite of traits that enable plants to accelerate the completion of their life cycle, thereby escaping late-season d...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1400557

    authors: Dudley LS,Hove AA,Emms SK,Verhoeven AS,Mazer SJ

    更新日期:2015-06-01 00:00:00

  • Distinct geographic structure as evidenced by chloroplast DNA haplotypes and ploidy level in Japanese Aucuba (Aucubaceae).

    abstract::The geographic distribution of diploid and tetraploid cytotypes and cpDNA haplotypes throughout the entire range of Aucuba japonica was investigated. We measured relative genome size using flow cytometry and sequenced two cpDNA intergenic regions, atpB-rbcL and psbA-trnH (GUG). Two haplotypes include both diploids and...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.90.11.1645

    authors: Ohi T,Kajita T,Murata J

    更新日期:2003-11-01 00:00:00

  • Ribosomal DNA phylogeny of the Bangiophycidae (Rhodophyta) and the origin of secondary plastids.

    abstract::We sequenced the nuclear small subunit ribosomal DNA coding region from 20 members of the Bangiophycidae and from two members of the Florideophycidae to gain insights into red algal evolution. A combined alignment of nuclear and plastid small subunit rDNA and a data set of Rubisco protein sequences were also studied t...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Müller KM,Oliveira MC,Sheath RG,Bhattacharya D

    更新日期:2001-08-01 00:00:00

  • Resilience to multiple stressors in an aquatic plant and its microbiome.

    abstract:PREMISE:Outcomes of species interactions, especially mutualisms, are notoriously dependent on environmental context, and environments are changing rapidly. Studies have investigated how mutualisms respond to or ameliorate anthropogenic environmental changes, but most have focused on nutrient pollution or climate change...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1404

    authors: O'Brien AM,Yu ZH,Luo DY,Laurich J,Passeport E,Frederickson ME

    更新日期:2020-02-01 00:00:00

  • Bioclimatic factors at an intrabiome scale are more limiting than cyanobiont availability for the lichen-forming genus Peltigera.

    abstract:PREMISE OF THE STUDY:Factors shaping spatiotemporal patterns of associations in mutualistic systems are poorly understood. We used the lichen-forming fungi Peltigera and their cyanobacterial partners Nostoc to investigate the spatial structure of this symbiosis at an intrabiome scale and to identify potential factors s...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1119

    authors: Lu J,Magain N,Miadlikowska J,Coyle JR,Truong C,Lutzoni F

    更新日期:2018-07-01 00:00:00

  • Placing the woody tropical genera of Polygonaceae: A hypothesis of character evolution and phylogeny.

    abstract:UNLABELLED: PREMISE OF THE STUDY:Taxonomic groups have often been recognized on the basis of geographic distinctions rather than accurately representing evolutionary relationships. This has been particularly true for temperate and tropical members from the same family. Polygonaceae exemplifies this problem, wherein th...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1000022

    authors: Burke JM,Sanchez A,Kron K,Luckow M

    更新日期:2010-08-01 00:00:00

  • Origin of the disjunct tetraploid Cardamine amporitana (Brassicaceae) assessed with nuclear and chloroplast DNA sequence data.

    abstract::Seventy-four nucleotide sequences from the ITS regions of nuclear ribosomal DNA and 76 from the trnL-trnF spacer of chloroplast DNA were used to address the origin of tetraploid Cardamine amporitana, the conspecifity of central Italian and northeastern Spanish populations, and the possible cause for such geographic di...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.91.8.1231

    authors: Lihová J,Fuertes Aguilar J,Marhold K,Nieto Feliner G

    更新日期:2004-08-01 00:00:00

  • Estimating near-infrared leaf reflectance from leaf structural characteristics.

    abstract::The relationship between near-infrared reflectance at 800 nm (NIRR) from leaves and characteristics of leaf structure known to affect photosynthesis was investigated in 48 species of alpine angiosperms. This wavelength was selected to discriminate the effects of leaf structure vs. chemical or water content on leaf ref...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Slaton MR,Raymond Hunt E Jr,Smith WK

    更新日期:2001-02-01 00:00:00

  • Genotype-specific effects of ericoid mycorrhizae on floral traits and reproduction in Vaccinium corymbosum.

    abstract:PREMISE:Most plants interact with mycorrhizal fungi and animal pollinators simultaneously. Yet, whether mycorrhizae affect traits important to pollination remains poorly understood and may depend on the match between host and fungal genotypes. Here, we examined how ericoid mycorrhizal fungi affected flowering phenology...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1372

    authors: Brody AK,Waterman B,Ricketts TH,Degrassi AL,González JB,Harris JM,Richardson LL

    更新日期:2019-11-01 00:00:00

  • Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae).

    abstract:PREMISE OF THE STUDY:We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. METHODS AND RESULTS:mRNA from an individual seedling was subjected to cDNA synthesis ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1100276

    authors: Sakaguchi S,Uchiyama K,Ueno S,Ujino-Ihara T,Tsumura Y,Prior LD,Bowman DM,Crisp MD,Isagi Y

    更新日期:2011-12-01 00:00:00

  • Predicting mating patterns from pollination syndromes: the case of "sapromyiophily" in Tacca chantrieri (Taccaceae).

    abstract::Tacca, a genus of tropical herbs, possesses near black flowers, conspicuous involucral bracts and whisker-like filiform bracteoles. These features have been assumed to function as a "deceit syndrome" in which reproductive structures resemble decaying organic material attracting flies that facilitate cross-pollination ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.92.3.517

    authors: Zhang L,Barrett SC,Gao JY,Chen J,Cole WW,Liu Y,Bai ZL,Li QJ

    更新日期:2005-03-01 00:00:00

  • New findings on the pollination biology of Mauritia flexuosa (Arecaceae) in Roraima, Brazil: linking dioecy, wind, and habitat.

    abstract:UNLABELLED: PREMISE OF THE STUDY:Despite the ecological, economic, and cultural importance of the palm Mauritia flexuosa in Amazonia, knowledge of its reproductive biology is scarce. Previous observations of this species suggested beetles as the probable pollinator, but experimental work to confirm this hypothesis was...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1200446

    authors: Khorsand Rosa R,Koptur S

    更新日期:2013-03-01 00:00:00

  • Hughmillerites vancouverensis sp. nov. and the Cretaceous diversification of Cupressaceae.

    abstract:UNLABELLED:• PREMISE OF THE STUDY:Two ovulate conifer cones, one of which is attached terminally to a short leafy shoot, reveal the presence of a new species of Hughmillerites in the Early Cretaceous Apple Bay flora of Vancouver Island, British Columbia, Canada. This ancient conifer expands the diversity of Cupressace...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1400369

    authors: Atkinson BA,Rothwell GW,Stockey RA

    更新日期:2014-12-01 00:00:00

  • Ecological factors influencing tetraploid establishment in snow buttercups (Ranunculus adoneus , Ranunculaceae): minority cytotype exclusion and barriers to triploid formation.

    abstract::Polyploid speciation is an ongoing, important source of angiosperm diversity. However, the barriers to polyploid speciation and mechanisms of establishment remain poorly understood for all but a few species. Several factors likely to have influenced tetraploid establishment, including barriers to triploid formation, c...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.92.11.1827

    authors: Baack EJ

    更新日期:2005-11-01 00:00:00

  • Development of microsatellite markers for Anadenanthera colubrina var. cebil (Fabaceae), a native tree from South America.

    abstract:PREMISE OF THE STUDY:Microsatellite primers were developed in the native legume tree Anadenanthera colubrina var. cebil to study the genetic diversity and genetic structure in natural populations in Argentina. METHODS AND RESULTS:Nine microsatellite markers were identified using a genomic library enriched for tandemly...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1200078

    authors: Barrandeguy ME,Prinz K,García MV,Finkeldey R

    更新日期:2012-09-01 00:00:00

  • Development of EST-SSR markers for Elaeocarpus photiniifolia (Elaeocarpaceae), an endemic taxon of the Bonin Islands.

    abstract:PREMISE OF THE STUDY:Expressed sequence tag (EST)-derived microsatellite markers were developed for Elaeocarpus photiniifolia, an endemic taxon of the Bonin Islands. METHODS AND RESULTS:Initially, a complementary DNA (cDNA) library was constructed by de novo pyrosequencing of total RNA extracted from a seedling. A tot...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1100391

    authors: Sugai K,Setsuko S,Uchiyama K,Murakami N,Kato H,Yoshimaru H

    更新日期:2012-02-01 00:00:00

  • Moving with climbing plants from Charles Darwin's time into the 21st century.

    abstract::We provide an overview of research on climbing plants from Charles Darwin to the present day. Following Darwin's interests, this review will focus on functional perspectives including attachment mechanisms and stem structure and function. We draw attention to a number of unsolved problems inviting future research. The...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.0900045

    authors: Isnard S,Silk WK

    更新日期:2009-07-01 00:00:00

  • Aborted fruits of Opuntia microdasys (Cactaceae): insurance against reproductive failure.

    abstract::New individuals in clonal populations arise through the recruitment of sexual or clonal offspring. The predominance of one type of regeneration over the other has been correlated with different selective environmental pressures. We compared the reproductive mode (sexual through seeds and vegetative through plantlets o...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.93.4.505

    authors: Palleiro N,Mandujano MC,Golubov J

    更新日期:2006-04-01 00:00:00

  • Strait of Gibraltar: an effective gene-flow barrier for wind-pollinated Carex helodes (Cyperaceae) as revealed by DNA sequences, AFLP, and cytogenetic variation.

    abstract::The Strait of Gibraltar is the most important barrier disconnecting the landmasses of Europe and Africa on the western Mediterranean extreme. Carex helodes is a wind-pollinated species endemic to the western Mediterranean. Because molecular and cytogenetic data allow the inference of its evolutionary history, we analy...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.2007342

    authors: Escudero M,Vargas P,Valcárcel V,Luceño M

    更新日期:2008-06-01 00:00:00

  • The pattern of carbon allocation supporting growth of preformed shoot primordia in Acomastylis rossii (Rosaceae).

    abstract::Extreme preformation, the initiation of leaves or inflorescences more than 1 yr before maturation and function, is common in arctic and alpine habitats. This extended pattern of development provides a potential means to alleviate an apparent asynchrony between carbon supplied by photosynthesis in the summer and carbon...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.90.9.1313

    authors: Meloche CG,Diggle PK

    更新日期:2003-09-01 00:00:00

  • Rediscovery of Pedilanthus coalcomanensis (Euphorbiaceae), a threatened endemic Mexican species.

    abstract::Pedilanthus coalcomanensis was described from specimens collected by George B. Hinton in 1941 but was not collected again until 1999, when we found it in a tropical deciduous forest near Tehuantepec, in Chinicuila, Michoacán, Mexico. After analyzing Hinton's original collection notes, we concluded that this is the typ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.89.9.1485

    authors: Lomelí-Sención JA,Sahagún-Godínez E

    更新日期:2002-09-01 00:00:00

  • A comparison of male and female responses to inbreeding in Cucurbita pepo subsp. texana (Cucurbitaceae).

    abstract::Accurate estimates of inbreeding depression are necessary in order to predict the evolutionary dynamics of a population, but many studies estimate inbreeding depression based solely on components of female function such as fruit set, seed set, and seed quality. Because total fitness is achieved through both male and f...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.92.1.107

    authors: Hayes CN,Winsor JA,Stephenson AG

    更新日期:2005-01-01 00:00:00

  • Does selfing or outcrossing promote local adaptation?

    abstract::The degree to which plants self-fertilize may impact their potential for genetic adaptation. Given that the mating system influences genetic processes within and among populations, the mating system could limit or promote local adaptation. I conducted a literature survey of published reciprocal transplant experiments ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.0900224

    authors: Hereford J

    更新日期:2010-02-01 00:00:00

  • Amplified fragment length polymorphisms (AFLP) reveal details of polyploid evolution in Dactylorhiza (Orchidaceae).

    abstract::The utility of the PCR-based AFLP technique (polymerase chain reaction; amplified fragment length polymorphisms) was explored in elucidating details of polyploid evolution in the Eurasian orchid genus Dactylorhiza. We emphasized Swedish taxa but also included some material from the British Isles and elsewhere in Europ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Hedrén M,Fay MF,Chase MW

    更新日期:2001-10-01 00:00:00

  • Geographic patterns in the reproductive ecology of Agave lechuguilla (Agavaceae) in the Chihuahuan desert. I. Floral characteristics, visitors, and fecundity.

    abstract::Floral characteristics such as morphology and flower color have been interpreted as adaptive traits that evolved through selective pressures generated by pollinators. Differences among populations in the expression of floral characters could result from natural selection for their adaptive value to local conditions. W...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.90.3.377

    authors: Silva-Montellano A,Eguiarte LE

    更新日期:2003-03-01 00:00:00

  • Wood vessel diameter is related to elevation and genotype in the Hawaiian tree Metrosideros polymorpha (Myrtaceae).

    abstract::We tested the hypothesis that trees growing at high elevations with occasional freezing temperatures have smaller diameter xylem vessels than trees of the same species growing at lower and warmer elevations. The young branch wood of the wide-ranging Hawaiian tree species Metrosideros polymorpha (Myrtaceae) was examine...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.94.5.709

    authors: Fisher JB,Goldstein G,Jones TJ,Cordell S

    更新日期:2007-05-01 00:00:00

  • Tracking temporal shifts in area, biomes, and pollinators in the radiation of Salvia (sages) across continents: leveraging anchored hybrid enrichment and targeted sequence data.

    abstract:PREMISE OF THE STUDY:A key question in evolutionary biology is why some clades are more successful by being widespread geographically, biome diverse, or species-rich. To extend understanding of how shifts in area, biomes, and pollinators impact diversification in plants, we examined the relationships of these shifts to...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.1002/ajb2.1268

    authors: Kriebel R,Drew BT,Drummond CP,González-Gallegos JG,Celep F,Mahdjoub MM,Rose JP,Xiang CL,Hu GX,Walker JB,Lemmon EM,Lemmon AR,Sytsma KJ

    更新日期:2019-04-01 00:00:00

  • The pollination ecology of buzz-pollinated Rhexia virginica (Melastomataceae).

    abstract::We examined the function of floral traits associated with buzz pollination through studies of Rhexia virginica (Melastomataceae) in the Muskoka region of Ontario, Canada. Controlled pollinations demonstrated that the species is self-compatible, but dependent on insects for pollen transfer. Bumble bees made 82 and 90% ...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:

    authors: Larson BM,Barrett SC

    更新日期:1999-04-01 00:00:00

  • Long-term shifts in the phenology of rare and endemic Rocky Mountain plants.

    abstract:UNLABELLED:• PREMISE OF THE STUDY:Mountainous regions support high plant productivity, diversity, and endemism, yet are highly vulnerable to climate change. Historical records and model predictions show increasing temperatures across high elevation regions including the Southern Rocky Mountains, which can have a stron...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1500156

    authors: Munson SM,Sher AA

    更新日期:2015-08-01 00:00:00

  • Phylogeny of palaeotropic Derris-like taxa (Fabaceae) based on chloroplast and nuclear DNA sequences shows reorganization of (infra)generic classifications is needed.

    abstract:PREMISE OF THE STUDY:Palaeotropic Derris-like taxa (family Fabaceae, tribe Millettieae) comprise 6-9 genera. They are well known as important sources of rotenone toxin, which are used as organic insecticide and fish poison. However, their phylogenetic relationships and classification are still problematic due to insuff...

    journal_title:American journal of botany

    pub_type: 杂志文章

    doi:10.3732/ajb.1200390

    authors: Sirichamorn Y,Adema FA,Gravendeel B,van Welzen PC

    更新日期:2012-11-01 00:00:00