Abstract:
UNLABELLED: PREMISE OF THE STUDY:Large-scale population genetics studies are required to investigate the dispersal processes underlying the emergence of Mycosphaerella fijiensis, a fungal pathogen of banana. To this end, we have developed an optimized genotyping procedure combining novel microsatellite markers and a modified DNA extraction protocol. • METHODS AND RESULTS:Primers for tetranucleotide loci were designed directly from the recently published genome sequence of M. fijiensis. A total of 19 new polymorphic and easy-to-score markers were developed. Their use was combined with an adapted protocol for total DNA extraction starting from young lesions collected from banana leaves, thus avoiding a pathogen isolation step. • CONCLUSIONS:The combination of the two technical developments presented here will permit the expansion of genotyping capacity in M. fijiensis, allowing large-scale analysis of samples from various geographic locations.
journal_name
Am J Botjournal_title
American journal of botanyauthors
Robert S,Rieux A,Argout X,Carlier J,Zapater MFdoi
10.3732/ajb.1000295subject
Has Abstractpub_date
2010-12-01 00:00:00pages
e130-2issue
12eissn
0002-9122issn
1537-2197pii
ajb.1000295journal_volume
97pub_type
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journal_title:American journal of botany
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更新日期:2014-12-01 00:00:00
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doi:10.3732/ajb.93.4.505
更新日期:2006-04-01 00:00:00
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更新日期:2001-10-01 00:00:00
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更新日期:2015-08-01 00:00:00
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