Abstract:
PREMISE OF THE STUDY:We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. METHODS AND RESULTS:mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism within 16 individuals representative of the species' entire range, with the number of alleles per locus and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively. CONCLUSIONS:The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation genetics and reproductive ecology of the species.
journal_name
Am J Botjournal_title
American journal of botanyauthors
Sakaguchi S,Uchiyama K,Ueno S,Ujino-Ihara T,Tsumura Y,Prior LD,Bowman DM,Crisp MD,Isagi Ydoi
10.3732/ajb.1100276subject
Has Abstractpub_date
2011-12-01 00:00:00pages
e363-8issue
12eissn
0002-9122issn
1537-2197pii
ajb.1100276journal_volume
98pub_type
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