Isolation and characterization of 52 polymorphic EST-SSR markers for Callitris columellaris (Cupressaceae).

Abstract:

PREMISE OF THE STUDY:We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan. METHODS AND RESULTS:mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism within 16 individuals representative of the species' entire range, with the number of alleles per locus and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively. CONCLUSIONS:The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation genetics and reproductive ecology of the species.

journal_name

Am J Bot

authors

Sakaguchi S,Uchiyama K,Ueno S,Ujino-Ihara T,Tsumura Y,Prior LD,Bowman DM,Crisp MD,Isagi Y

doi

10.3732/ajb.1100276

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

e363-8

issue

12

eissn

0002-9122

issn

1537-2197

pii

ajb.1100276

journal_volume

98

pub_type

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