Abstract：
AIMS:17β-Oestradiol (E2) and its receptors (ERα and ERβ) are important regulators of physiological and pathological processes in the cardiovascular system. ER act in concert with other regulatory factors mediating oestrogenic effects. However, the underlying mechanisms modulating ER transcriptional activity are not fully elucidated. To gain better understanding of E2-induced ERα action in the human heart, we aimed to identify and functionally analyse interaction partners of ERα. METHODS AND RESULTS:Using yeast two-hybrid assays with a human heart cDNA library, we identified atrial natriuretic peptide precursor A (NPPA), a well-known cardiac hypertrophy marker, as a novel ERα interaction partner interacting in an E2-dependent manner. Mutation analyses and immunofluorescence data indicated that the LXXLL motif within NPPA is necessary for its E2-induced interaction with ERα, its action as a co-repressor of ERα, and its translocation into the nucleus of human and rat cardiomyocytes. Expression analysis and chromatin immunoprecipitation assays in a human left ventricular cardiomyocyte cell line, AC16, showed that NPPA interacts with E2/ERα, suppressing the transcriptional activity of ERα on E2-target genes, such as NPPA, connexin43, αactinin-2, nuclear factor of activated T-cells, and collagens I and III. CONCLUSION:We characterize for the first time an E2-regulated interaction of NPPA with ERα in cardiomyocytes, that may be crucial in physiological and/or pathological cardiac processes, thereby representing a potential therapeutic target.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Mahmoodzadeh S,Pham TH,Kuehne A,Fielitz B,Dworatzek E,Kararigas G,Petrov G,Davidson MM,Regitz-Zagrosek Vdoi
10.1093/cvr/cvs281subject
Has Abstractpub_date
2012-12-01 00:00:00pages
411-21issue
3eissn
0008-6363issn
1755-3245pii
cvs281journal_volume
96pub_type
杂志文章abstract：:The mechanisms whereby glucose reduces fatty acid-induced release of enzyme from the coronary-ligated isolated perfused working rat heart are investigated. Alterations in the tissue contents of ATP, phosphocreatine, or glycogen could be excluded as possible mechanisms for the beneficial effect of glucose in this syst...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.12.693
更新日期：1979-12-01 00:00:00
abstract：OBJECTIVE:To determine the effect of acute left ventricular dilatation on refractoriness in normal hearts. METHODS:During sustained ventricular fibrillation (VF) in isolated perfused hearts, recording of local activation time yields VF intervals which provide an index of local refractoriness. Simultaneous measurement ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(98)00092-3
更新日期：1998-08-01 00:00:00
abstract：OBJECTIVE:We propose that ischemic preconditioning (IPC) and mitochondrial K(ATP) channel activation protect the myocardium by inhibiting mitochondrial permeability transition pore (MPTP) opening at reperfusion. METHODS:Isolated rat hearts were subjected to 35 min ischemia/120 min reperfusion and assigned to the follo...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(02)00455-8
更新日期：2002-08-15 00:00:00
abstract：:The mechanical, haemodynamic, and energetic functions of isolated perfused working hearts from chronic alcoholic and control rats were studied. Male Long-Evans rats were fed a nutritionally-complete liquid diet containing 38% of daily calories as ethanol (isocaloric replacement with dextrin-maltose for controls) for 3...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/13.3.136
更新日期：1979-03-01 00:00:00
abstract：:Atherosclerosis is considered to be a form of chronic inflammation governed by a complex network of inter- and intra-cellular signaling pathways. The pleiotropic cytokine interferon-gamma (IFN-gamma) is a key pro-inflammatory mediator that is expressed at high levels in atherosclerotic lesions. IFN-gamma regulates the...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/j.cardiores.2005.04.019
更新日期：2005-07-01 00:00:00
abstract：:Changes in left ventricular internal diameter resulting from increasing intrapericardial pressure induced by increasing the pericardial fluid volume were studied in closed chest, anaesthetized dogs. Left ventricular internal diameters were measured by placing an ultrasonic dimension catheter in the left ventricle at t...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/9.6.707
更新日期：1975-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to determine whether chronic beta-adrenergic receptor (beta-AR) stimulation induces proliferation of human cardiac fibroblasts and to investigate the mechanism(s) involved. METHODS AND RESULTS:In vitro cultures of human cardiac fibroblasts were established from biopsies of right atr...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(02)00729-0
更新日期：2003-03-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of the ATP-sensitive potassium (KATP) channel inhibitor sodium 5-hydroxydecanoate (5-HD) on the reduction in myocardial infarct size afforded by ischaemic preconditioning in the sodium pentobarbitone rabbit. METHODS:New Zealand white rabbits were anaesthetised with sodium pentobarbi...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：OBJECTIVE:In many species arrhythmias induced by myocardial ischaemia appear to be in part dependent upon cardiac sympathetic nerves. However, previous experiments in rats did not suggest that myocardial or other catecholamines are involved in ischaemic arrhythmogenesis in this species. The aim of this study was to inv...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(99)00103-0
更新日期：1999-09-01 00:00:00
abstract：OBJECTIVE:In human heart failure beta-adrenergic-mediated protein kinase A (PKA) activity is down-regulated, while protein kinase C (PKC) activity is up-regulated. PKC-mediated myofilament protein phosphorylation might be detrimental for contractile function in cardiomyopathy. This study was designed to reveal the effe...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/j.cardiores.2005.11.021
更新日期：2006-03-01 00:00:00
abstract：:The cardiac SR Ca(2+)-ATPase (SERCA2a) regulates intracellular Ca(2+)-handling and thus, plays a crucial role in initiating cardiac contraction and relaxation. SERCA2a may be modulated through its accessory phosphoprotein phospholamban or by direct phosphorylation through Ca(2+)/calmodulin dependent protein kinase II ...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(02)00694-6
更新日期：2003-01-01 00:00:00
abstract：Aims:Endothelial hyperpermeability exacerbates multiple organ damage during inflammation or infection. The endothelial glycocalyx, a protective matrix covering the luminal surface of endothelial cells (ECs), undergoes enzymatic shedding during inflammation, contributing to barrier hyperpermeability. A disintegrin and m...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvy167
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVES:The present study was designed to determine whether tonic nitric oxide (NO)-mediated vasodilator innervation participates in basilar arterial dilatation in the anesthetized Japanese monkey. METHODS:The basilar arterial diameter was angiographically measured, and NG-nitro-L-arginine (L-NNA), a nitric oxide s...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：OBJECTIVE:The aim was to investigate how platelet activation during myocardial ischaemia can induce electrophysiological and arrhythmogenic effects, and examine the involvement of different platelet membrane receptors in producing these effects. METHODS:Transmembrane action potentials and electrograms were recorded fr...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/28.11.1662
更新日期：1994-11-01 00:00:00
abstract：:Severe sepsis and septic shock, which are among the most common causes of death in intensive care units worldwide, cause high morbidity, mortality, and social and economic costs. Therefore, developing successful therapies against sepsis is one of the most important challenges in critical care medicine. Death from sept...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvq317
更新日期：2011-02-15 00:00:00
abstract：:Cardiomyocytes express a surprisingly large number of potassium channel types. The primary physiological functions of the currents conducted by these channels are to maintain the resting membrane potential and mediate action potential repolarization under basal conditions and in response to changes in the concentratio...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvaa068
更新日期：2020-07-15 00:00:00
abstract：:The static mechanical properties of major branches of the human arteries (common carotid artery, abdominal aorta, femoral artery, and brachial artery) were studied in 39 subjects, aged 6-81 years, using an ultrasonic phase locked echo tracking system that allows continuous transcutaneous measurement of the diameter of...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/21.9.678
更新日期：1987-09-01 00:00:00
abstract：:This study is the first report describing the sequence of biochemical alterations of myocardium during the progression of naturally occurring hypertrophy in spontaneously hypertensive rats (SHRs) and then with its reversal by alpha-methyldopa therapy. Changes in DNA, RNA, hydroxyproline, as well as incorporation of 14...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/10.2.254
更新日期：1976-03-01 00:00:00
abstract：AIMS:The aim of this work was to use hyperpolarized carbon-13 ((13)C) magnetic resonance (MR) spectroscopy and cine MR imaging (MRI) to assess in vivo cardiac metabolism and function in the 15-week-old spontaneously hypertensive rat (SHR) heart. At this time point, the SHR displays hypertension and concentric hypertrop...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvs164
更新日期：2012-07-01 00:00:00
abstract：Aims:MicroRNAs (miRNAs) have been implicated in the pathogenesis of pulmonary hypertension (PH), a multifactorial and progressive condition associated with an increased afterload of the right ventricle leading to heart failure and death. The main aim of this study was to correlate the levels of miR-424(322) with the se...
journal_title：Cardiovascular research
pub_type： 杂志文章,多中心研究
doi：10.1093/cvr/cvx187
更新日期：2018-01-01 00:00:00
abstract：AIMS:Sleep disturbances exhibit a strong social patterning, and inadequate sleep has been associated with adverse health outcomes, including cardiovascular disorders (CVD). However, the contribution of sleep to socioeconomic inequalities in CVD is unclear. This study pools data from eight European cohorts to investigat...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvz267
更新日期：2020-07-01 00:00:00
abstract：:Numerous epidemiological studies documented an inverse relationship between plasma high-density lipoprotein (HDL) cholesterol levels and the extent of atherosclerotic disease. However, clinical interventions targeting HDL cholesterol failed to show clinical benefits with respect to cardiovascular risk reduction, sugge...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1093/cvr/cvu136
更新日期：2014-08-01 00:00:00
abstract：AIMS:KCNQ1 (alias KvLQT1 or Kv7.1) and KCNE1 (alias IsK or minK) co-assemble to form the voltage-activated K(+) channel responsible for I(Ks)-a major repolarizing current in the human heart-and their dysfunction promotes cardiac arrhythmias. The channel is a component of larger macromolecular complexes containing known...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvn085
更新日期：2008-08-01 00:00:00
abstract：:The major limitation to antiarrhythmic drug therapy is the risk of arrhythmia promotion, or 'proarrhythmia.' This complication may be lethal, and greatly restricts the value of antiarrhythmic agents, particularly for arrhythmias without an intrinsic mortality risk, such as atrial fibrillation. In order for improved an...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(97)00293-9
更新日期：1998-03-01 00:00:00
abstract：:Exogenous angiotensin II causes noradrenaline supersensitivity in rat mesenteric vasculature preparations. The noradrenaline supersensitivity of tissues from renal/salt hypertensive rats, with low plasma renin activity, is not caused by endogenous angiotensin II since it was unaffected by Sar1 Ileu8 angiotensin II. Di...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/10.2.232
更新日期：1976-03-01 00:00:00
abstract：OBJECTIVE:Jervell and Lange-Nielsen syndrome (JLNS) is a recessively inherited long QT syndrome (LQTS) characterised by profound sensorineural deafness and predisposition to syncope and sudden cardiac death. Mutation analysis has established the presence of mutations in affected individuals in the genes KCNQ1 and KCNE1...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(01)00350-9
更新日期：2001-09-01 00:00:00
abstract：:Angiotensin II (AII) plays a critical role in cardiac remodeling. This peptide promotes cardiac myocyte hypertrophy and cardiac fibroblast interstitial fibrotic changes associated with left ventricular hypertrophy, post myocardial infarction remodeling and congestive heart failure. AII mediates cardiac myocyte hypertr...
journal_title：Cardiovascular research
pub_type： 杂志文章,评审
doi：10.1016/s0008-6363(00)00044-4
更新日期：2000-05-01 00:00:00
abstract：OBJECTIVE:This study tested the hypothesis that overproduction of endogenous nitric oxide (NO) during endotoxemia may modulate coronary autoregulation and myocardial reactive hyperemia. METHODS:Hearts of endotoxin-pretreated rats and controls were isolated and arranged for perfusion in a Langendorff preparation. Autor...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(97)00132-6
更新日期：1997-08-01 00:00:00
abstract：BACKGROUND:In the myocardium, myocyte cell division is irreversibly blocked shortly after birth. The signal that initiates cell cycle withdrawal is unknown. The purpose of this study was to relate changes in expression of beta1 integrin and its associated alpha subunits to cardiomyocyte cell cycle progression during th...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1016/s0008-6363(00)00140-1
更新日期：2000-09-01 00:00:00
abstract：AIMS:Hyperhomocysteinaemia is an independent risk factor for atherosclerotic vascular disease and is associated with vascular endothelial dysfunction. Homocysteine modulates cellular methylation reactions. P66shc is a protein that promotes oxidative stress whose expression is governed by promoter methylation. We asked ...
journal_title：Cardiovascular research
pub_type： 杂志文章
doi：10.1093/cvr/cvr250
更新日期：2011-12-01 00:00:00
