Abstract:
BACKGROUND:Adenosine monophosphate (AMP)-activated protein kinase (AMPK), as a sensor of cellular energy status, has been known to play an important role in the pathophysiology of diabetes and its complications. As AMPK is also expressed in podocytes, it is possible that podocyte AMPK would be an important contributing factor in the development of diabetic proteinuria. We investigated the roles of AMPK in the pathological changes of podocytes induced by angiotensin II (Ang II), a major injury inducer in diabetic proteinuria. METHODS:Mouse podocytes were incubated in media containing various concentrations of Ang II and AMPK-modulating agents. The changes of AMPKα were analyzed by confocal imaging and Western blotting in response to Ang II. RESULTS:Ang II changed the localization of AMPKα from peripheral cytoplasm into internal cytoplasm and peri- and intranuclear areas in podocytes. Ang II also reduced AMPKα (Thr172) phosphorylation in time- and dose-sensitive manners. In particular, 10(-7 )M Ang II reduced phospho-AMPKα significantly and continuously at 6, 24, and 48 h. AMPK activators, metformin and 5-aminoimidazole-4-carboxamide-1β-riboside, restored the suppressed AMPKα (Thr172) phosphorylation. Losartan, an Ang II type 1 receptor antagonist, also recovered the suppression and the mal-localization of AMPKα, which were induced by Ang II. PD98059, a mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor, also restored the AMPKα (Thr172) phosphorylation suppressed by Ang II. CONCLUSION:We suggest that Ang II induces the relocation and suppression of podocyte AMPKα via Ang II type 1 receptor and MAPK signaling pathway, which would be an important mechanism in Ang II-induced podocyte injury.
journal_name
Clin Exp Nephroljournal_title
Clinical and experimental nephrologyauthors
Choi JY,Ha TS,Park HY,Ahn HYdoi
10.1007/s10157-012-0649-8subject
Has Abstractpub_date
2013-02-01 00:00:00pages
16-23issue
1eissn
1342-1751issn
1437-7799journal_volume
17pub_type
杂志文章abstract:BACKGROUND:Analyses of selected cases suggest that immunosuppressive treatment could reduce proteinuria and delay the progression of immunoglobulin A nephropathy (IgAN). The aim of this study was to examine the long-term effectiveness of this therapy on the clinical course of IgAN. We also examined the relationship bet...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-008-0045-6
更新日期:2008-08-01 00:00:00
abstract::The clinical course of cryoglobulinemic glomerulonephritis (CGGN) is usually slowly progressive, and only a minority of these patients progress to end-stage renal failure. This report describes an atypical case of a patient with CGGN who demonstrated a rapidly progressive and irreversible renal deterioration. After pr...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-010-0301-4
更新日期:2010-10-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-004-0303-1
更新日期:2004-09-01 00:00:00
abstract::A 61-year-old man infected with hepatitis C virus developed urinary protein. Two-dimensional electrophoresis and immunoblotting of sera revealed no monoclonal proteins. Light microscopy and immunofluorescence of a kidney biopsy specimen demonstrated bubbling appearance and formation of spikes, associated with predomin...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-009-0227-x
更新日期:2010-02-01 00:00:00
abstract::Recent studies on dendritic cells (DCs) and macrophages have greatly advanced our knowledge of glomerular immunopathology. This rapidly developing field most likely has considerable impact on our understanding of the major mediators of tissue injury and repair in kidney disease. The pivotal role of cytokine in the ini...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章,评审
doi:10.1007/s10157-009-0218-y
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abstract:BACKGROUND:In order to clarify the interaction between cardiac dysfunction and sodium homeostasis in the kidney, we used a murine model of cardiac dysfunction and investigated the effect on sodium transporters in renal tubular cells. METHODS:Cardiac function was deteriorated by abdominal aortic banding, and the gene e...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-013-0820-x
更新日期:2014-02-01 00:00:00
abstract::Inflammatory pseudotumour (IPT) is a rare disease of unknown cause that most commonly involves the lung but can occur in almost any site in the body. Occurrence in the kidneys is very rare and bilateral renal involvement even rarer. There are 34 previously reported cases in the English-language medical literature betw...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-009-0242-y
更新日期:2010-04-01 00:00:00
abstract::We describe here two sisters with nephronophthisis, which was not detected until the development of endstage renal failure. Twenty- and 15-year-old female siblings were admitted to our hospital for further examination of renal dysfunction. No urinalysis abnormalities had been found in yearly health checks in either pa...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-005-0377-4
更新日期:2005-12-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-018-1628-5
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journal_title:Clinical and experimental nephrology
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章,评审
doi:10.1007/s10157-009-0252-9
更新日期:2010-06-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章,评审
doi:10.1007/s10157-013-0891-8
更新日期:2014-04-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-014-1036-4
更新日期:2015-08-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-013-0802-z
更新日期:2014-02-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-012-0665-8
更新日期:2013-02-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-003-0267-6
更新日期:2004-03-01 00:00:00
abstract:BACKGROUND:The current (2012) histological classification of immunoglobulin A nephropathy was established using a case-control study of 287 patients. However, the risk of progression to end-stage renal disease (ESRD) has not been validated for the previous (2002) classification. This study aimed to determine whether th...
journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-013-0848-y
更新日期:2014-06-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-014-0967-0
更新日期:2015-04-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章,多中心研究
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journal_title:Clinical and experimental nephrology
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更新日期:2012-02-01 00:00:00
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更新日期:2020-02-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2013-12-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-020-02009-0
更新日期:2021-01-20 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-018-1666-z
更新日期:2019-04-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-013-0841-5
更新日期:2014-06-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-020-02006-3
更新日期:2021-01-04 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-008-0042-9
更新日期:2008-08-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-009-0260-9
更新日期:2010-04-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-010-0342-8
更新日期:2011-02-01 00:00:00
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journal_title:Clinical and experimental nephrology
pub_type: 杂志文章
doi:10.1007/s10157-016-1374-5
更新日期:2017-10-01 00:00:00