Abstract:
:In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA or RNA strand to localize a specific DNA or RNA sequence on a chromosome or section of tissue (in-situ) fixed on a slide. Flourescence in-situ hybridization (FISH) technique facilitates the localization of genes to different chromosomal locations. It is extensively applied as a gene mapping tool for identification and validation of cytogenetic aberrations identified through comparative genomic hybridization (CGH) on large cohort of archival samples in a tissue microarray format. The discovery of cytogenetic aberrations in cancer has led to the development of quite a few FDA approved molecularly targeted drugs for the management of patients undergoing cancer treatment. FISH technique is extensively utilized as a predictor of responsiveness to treatment with targeted inhibitors, residual disease monitoring and also in noninvasive methods for the detection of tumor cells. Furthermore detection of circulating tumor cells can be detected which have metastatic potential with poor survival prospects. With the development of high throughput technologies like comparative genomic hybridization CGH and next-generation DNA sequencing, human pathology archival specimens of human tumors in various stages of development, can be utilized in the post-human-genome-sequencing era to obtain diagnostic and therapeutic guidance. This article will discuss the extensive application of FISH in diagnosis, prognosis and therapeutic monitoring of cancer.
journal_name
Curr Med Chemjournal_title
Current medicinal chemistryauthors
Jehan Z,Uddin S,Al-Kuraya KSdoi
10.2174/092986712801661031subject
Has Abstractpub_date
2012-01-01 00:00:00pages
3730-8issue
22eissn
0929-8673issn
1875-533Xpii
CMC-EPUB-20120607-20journal_volume
19pub_type
杂志文章,评审abstract::Among naturally occurring isoflavones, soy isoflavones are an important class with various biological activities. Due to their phytoestrogenic structure, their effects on the brain are profound thus making the neurobiological effects of these compounds an active area of research. One such compound is daidzein, which h...
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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更新日期:2014-01-01 00:00:00
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journal_title:Current medicinal chemistry
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更新日期:2013-01-01 00:00:00
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更新日期:2012-01-01 00:00:00
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journal_title:Current medicinal chemistry
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更新日期:2010-01-01 00:00:00
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journal_title:Current medicinal chemistry
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更新日期:2012-01-01 00:00:00
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journal_title:Current medicinal chemistry
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更新日期:2004-04-01 00:00:00
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更新日期:2011-01-01 00:00:00
abstract::There is evidence that hyperglycemia results in the generation of reactive oxygen species, leading to oxidative stress in various tissues, including vascular system. An important link between oxidative stress, inflammatory response and insulin activity is now well established. The ability of antioxidants to protect ag...
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更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:Chronic, degenerative diseases are often characterized by inflammation and aberrant angiogenesis. For these pathologies, including rheumatoid arthritis, cardiovascular and autoimmune diseases, cancer, diabetes, and obesity, current therapies have limited efficacy. OBJECTIVES:The validation of novel (chemo)p...
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更新日期:2019-01-01 00:00:00
abstract::Over the past few years, use of the genome-wide association study (GWAS) has made it possible to identify the primary genetic mechanisms of essential hypertension. GWAS results have helped identify many loci in or near genes that generally were not expected to be associated with blood pressure or essential hypertensio...
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更新日期:2015-01-01 00:00:00
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journal_title:Current medicinal chemistry
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abstract::Serotonin (5-hydroxytryptamine, 5-HT) is one of the most attractive targets for medicinal chemists. Among 5-HTRs, the 5-HT(1A) subtype is the best studied and it is generally accepted that it is involved in psychiatric disorders such as anxiety and depression. Several structurally different compounds are known to bind...
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journal_title:Current medicinal chemistry
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更新日期:2015-05-13 00:00:00
abstract::This research evaluated the effect of multiple-wave lasertherapy on the healing process of surgical wounds based on in vitro models denominated stem-dermal equivalents. These human skin models were obtained from a co-culture of dermal cells and bone marrow mesenchymal stem cells. The experimental tests were carried ou...
journal_title:Current medicinal chemistry
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更新日期:2012-01-01 00:00:00
abstract::Osteogenic peptides are, or have potential to be, therapies for the treatment of osteoporosis, fracture repair, and repair of loosened bone implants. Human parathyroid hormone has been approved for the treatment of post-menopausal osteoporosis. Constrained analogs of PTH and the parathyroid-hormone related peptide (PT...
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abstract::Epigenetics is a field of biological sciences focused on the study of reversible, heritable changes in gene function not due to modifications of the genomic sequence. These changes are the result of a complex cross-talk between several molecular mechanisms, that is in turn orchestrated by genetic and environmental fac...
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更新日期:2020-11-17 00:00:00
abstract::Hereditary amyloidogenic transthyretin (TTR) (ATTR) amyloidosis is an autosomal dominant form of fatal hereditary amyloidosis. Owing to progress in biochemical and molecular genetic analyses, this disease is now believed to occur worldwide. As of today, reports of about 120 different points of single or double mutatio...
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更新日期:2012-01-01 00:00:00
abstract::Since its discovery in 1995 adiponectin has garnered considerable interest from the academic, clinical and biotech communities due to its proposed salutary anti-inflammatory, anti-diabetic, anti-atherogenic and cardioprotective properties. As a result our appreciation of adiponectin's structure and the importance of p...
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更新日期:2012-01-01 00:00:00
abstract::The mitotic cell cycle is a tightly regulated process that ensures the correct division of one cell into two daughter cells. Progress along the different phases of the cell cycle is positively regulated by the sequential activation of a family of serine-threonine kinases called CDKs (Cyclin Dependent Kinases). Their a...
journal_title:Current medicinal chemistry
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更新日期:2005-01-01 00:00:00
abstract::Neurodegenerative disorders (NDDs) like Alzheimer's disease, Parkinson's disease and Huntington's disease are a heterogeneous group of disorders with the progressive and severe loss of neurons. There are no full proof cures for these diseases, and only medicines are available that can alleviate some of the symptoms. D...
journal_title:Current medicinal chemistry
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更新日期:2018-01-01 00:00:00
abstract::Thrombomodulin (TM) is a membrane protein mainly expressed by endothelial cells. It is part of the anticoagulant protein C system but recently several effects were discovered which occur independently of protein C activation. TM binds thrombin and promotes the cleavage of protein C and the thrombin activatable fibrino...
journal_title:Current medicinal chemistry
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更新日期:2014-01-01 00:00:00