Abstract：
:Macroautophagy is a dynamic process whereby cytoplasmic components are initially sequestered within autophagosomes. Recent studies have shown that the autophagosome membrane can selectively recognize ubiquitinated proteins and organelles through interaction with adapter proteins such as p62 and NBR1. Both proteins are structurally similar at the amino acid level, and bind with ubiquitin and ubiquitinated proteins. Although p62 is incorporated into a wide spectrum of pathological inclusions in various neurodegenerative diseases, abnormalities of NBR1 have not been reported in these diseases. Our immunohistochemical examination revealed that the vast majority of Lewy bodies (LBs) in Parkinson's disease and dementia with LBs (DLB) as well as of glial cytoplasmic inclusions in multiple system atrophy (MSA) were positive for NBR1. Neuronal and glial inclusions in tauopathies and TAR DNA-binding protein of 43 kDa proteinopathies were rarely immunolabeled, or were unstained. Using cultured cells bearing LB-like inclusions, formation of α-synuclein aggregates was repressed in cells with NBR1 knockdown. Immunoblot analysis showed that the level of NBR1 was significantly increased by 2.5-fold in MSA, but not in DLB. These findings suggest that NBR1 is involved in the formation of cytoplasmic inclusions in α-synucleinopathy.
journal_name
Acta Neuropatholjournal_title
Acta neuropathologicaauthors
Odagiri S,Tanji K,Mori F,Kakita A,Takahashi H,Wakabayashi Kdoi
10.1007/s00401-012-0975-7subject
Has Abstractpub_date
2012-08-01 00:00:00pages
173-86issue
2eissn
0001-6322issn
1432-0533journal_volume
124pub_type
杂志文章abstract：:An in vivo method for positively staining dead neurons was developed and compared with an in vitro staining method using acid fuchsin. Neurons previously killed by intracerebral injections of kainic acid were selectively stained by trypan blue within 15 min of its injection in vivo into the central nervous system of r...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00687254
更新日期：1989-01-01 00:00:00
abstract：:Data on the dural invasiveness of pituitary adenomas have been correlated to the expression of matrix metalloproteinases (e.g. MMP-9). Serine proteases have not yet been investigated in human pituitary adenomas. In this study, paraffin-embedded material from 84 human pituitary adenomas (acromegaly n=18, Cushing's dise...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-003-0747-5
更新日期：2003-11-01 00:00:00
abstract：:Twenty necropsy cases of the association of fetal encephalitis with porencephaly, hydranencephaly or polymicrogyria were reviewed including 5 from the authors' material. The latter include a basket brain, a porencephalic necrosis of recent date and a polymicrogyria in the formative state. The supratentorial lesions ar...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00685011
更新日期：1978-08-07 00:00:00
abstract：:Cornelia de Lange syndrome (CDLS) is a rare multisystemic malformative syndrome of uncertain etiology characterized by severe psychomotor and mental retardation. Here we report the neuropathological analysis of a 35-year-old patient who displayed the classical clinical symptomatology of CDLS. A congenital dysgenesis o...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-002-0562-4
更新日期：2002-09-01 00:00:00
abstract：:The nuclei for the nerves of a dorsal (m. splenius) and a ventral (m. longus capitis) neck muscle of the rat were retrogradely labeled by applying horseradish peroxidase (HRP) to the respective cut muscle nerves. Motoneurons of both muscles were analyzed for their localization, diameter of perikarya, and area of dendr...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00690368
更新日期：1981-01-01 00:00:00
abstract：:Flow cytofluorometric analysis was used to determine the distribution of the DNA content in cells from selected areas of normal human brain and in benign and malignant brain tumors. Propidium iodide was employed as DNA fluorochrome and the analysis was carried out on a suspension of single cells. Normal, nonstimulated...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00684802
更新日期：1979-04-12 00:00:00
abstract：:Spinal cord-notochord relationship was analyzed histologically and immunohistochemically in normal human conceptuses between the 4-8 developmental weeks and in a 8-week embryo with double spinal cord. In the early 4-week embryo, the gradual closure of the neural tube along the cranio-caudal body axis was paralleled by...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00228587
更新日期：1993-01-01 00:00:00
abstract：:Eleven atypical teratoid/rhabdoid tumors (AT/RT) and 121 primitive neuroectodermal tumors/medulloblastomas (PNET/MB) were included in this study for evaluation of the histopathological features of AT/RT and comparison between AT/RT and PNET/MB. Histopathological studies of AT/RT showed that in addition to the commonly...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s004010051149
更新日期：2000-05-01 00:00:00
abstract：:Amyloid-beta (Abeta) peptide pathology in Alzheimer's disease (AD) comprises extracellular plaques and cerebral amyloid angiopathy (CAA). In Parkinson's disease (PD), alpha-synuclein forms intraneuronal Lewy bodies (LBs), and cortical LBs are thought to play a major role in cognitive decline designated as PD with deme...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-007-0336-0
更新日期：2008-04-01 00:00:00
abstract：:We report the case of an elderly woman with a history of headache, vomiting and dizziness while walking. On CT scans a mass was identified in the right cerebellar hemisphere exhibiting radiological characteristics of lipomatous tissue. Surgery revealed a compact lesion consisting of whitish-yellow tissue with a fatty ...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00389504
更新日期：1994-01-01 00:00:00
abstract：:Huntington's disease (HD) is an autosomal dominant neurodegenerative disease caused by an expanded trinucleotide CAG repeat in the gene coding for huntingtin. Deregulation of chromatin remodeling is linked to the pathogenesis of HD but the mechanism remains elusive. To identify what genes are deregulated by trimethyla...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-013-1103-z
更新日期：2013-05-01 00:00:00
abstract：:We describe the clinical and neuropathological findings of three unrelated autopsy cases of MELAS harboring the A3243G transition in the mitochondrial DNA (mtDNA). Using immunohistochemical techniques, we studied the expression of several subunits of the respiratory chain in various brain regions from the same cases. ...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-003-0716-z
更新日期：2003-09-01 00:00:00
abstract：:Using a 3H-labelled virion DNA probe applied to tissue sections, we have previously identified the precise microscopic anatomical location of herpes simplex virus (HSV) during the acute and latent stages of infection of the mouse trigeminal ganglia and central nervous system (CNS). In the present investigation, we com...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00692842
更新日期：1987-01-01 00:00:00
abstract：:Seizures commonly occur in glioma patients, but their pathogenesis is poorly understood, in part due to a lack of valid and versatile experimental models. We have established a new model that enables comprehensive neuropathological and neurophysiological analysis on identical tissue preparations. Rat C6 glioma cells s...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-003-0771-5
更新日期：2004-01-01 00:00:00
abstract：:Cerebral β-amyloidosis can be exogenously induced by the intracerebral injection of brain extracts containing aggregated β-amyloid (Aβ) into young, pre-depositing Aβ precursor protein- (APP) transgenic mice. Previous work has shown that the induction involves a prion-like seeding mechanism in which the seeding agent i...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-014-1339-2
更新日期：2014-10-01 00:00:00
abstract：:Frontotemporal dementia (FTD) is a prevalent neurodegenerative disease of heterogeneous histopathology. Neuropathological subtypes are identified on the basis of the presence or absence of tau- or ubiquitin-positive neuronal inclusions. Our recent work has established four disease stages that are independent of neurop...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-004-0917-0
更新日期：2004-12-01 00:00:00
abstract：:By unknown mechanisms, the symptoms of amyotrophic lateral sclerosis (ALS) seem to spread along neuroanatomical pathways to engulf the motor nervous system. The rate at which symptoms spread is one of the primary drivers of disease progression. One mechanism by which ALS symptoms could spread is by a prion-like propag...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-014-1342-7
更新日期：2014-12-01 00:00:00
abstract：:A giant-cell glioblastoma was examined by electron microscopy and by the freeze-fracture technique. The cell membranes bordering the extensive extracellular space often showed complicated undulations and peripheral vacuoles as well as occasional microvilli or filopodia. The undulations were mainly composed of plasmale...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00689558
更新日期：1978-01-19 00:00:00
abstract：:Collections of human postmortem brains gathered in brain banks have underpinned many significant developments in the understanding of central nervous system (CNS) disorders and continue to support current research. Unfortunately, the worldwide decline in postmortem examinations has had an adverse effect on research ti...
journal_title：Acta neuropathologica
pub_type： 杂志文章,评审
doi：10.1007/s00401-008-0360-8
更新日期：2008-05-01 00:00:00
abstract：:To clarify the role of prostaglandins in peripheral nerve demyelination, we examined the expression of cyclooxygenase-2 (COX-2) using selected nerve specimens from patients with chronic inflammatory demyelinating polyneuropathy. COX-2 protein was up-regulated in macrophages causing active demyelination. In situ hybrid...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s004010000279
更新日期：2001-02-01 00:00:00
abstract：:Gelatinase A is an enzyme capable of cleaving soluble beta-amyloid protein (beta AP), and may function as an alpha-secretase to produce secretory forms of amyloid precursor protein. We examined gelatinase A immunoreactivity in the brains and posterior roots of neurologically normal, lacunar stroke, Alzheimer disease (...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00309334
更新日期：1995-01-01 00:00:00
abstract：:Wistar rats were given intravenous (i.v.) horseradish peroxidase (HRP) in saline in doses commonly used to study vascular permeability and the blood-brain barrier. Samples of blood were taken from conscious animals via indwelling catheters at intervals up to 6 h after the HRP injection. Plasma concentrations of adreno...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00687945
更新日期：1986-01-01 00:00:00
abstract：:This ultrastructural study deals with the synapses of primary dendrites of the anterior horn neurons in the lower lumbar spinal cords of seven patients with amyotrophic lateral sclerosis (ALS) who had mild neuronal depletion. Specimens from seven age-matched, neurologically normal individuals served as controls. In ea...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s004010050426
更新日期：1996-01-01 00:00:00
abstract：:The pattern of recovery of myelinated axons in the posterior tibial nerve after crushing was studied in rats chronically intoxicated with 2,5-hexanedione. It was given for 2 weeks before crushing (200 mg/kg i.p. 5 times a week) or additionally for two further weeks after the nerve crush. Two animals were examined from...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00703206
更新日期：1983-01-01 00:00:00
abstract：:Solanezumab and Crenezumab are two humanized antibodies targeting Amyloid-β (Aβ) which are currently tested in multiple clinical trials for the prevention of Alzheimer's disease. However, there is a scientific discussion ongoing about the target engagement of these antibodies. Here, we report the immunohistochemical s...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-015-1489-x
更新日期：2015-11-01 00:00:00
abstract：:We studied a case of primary leptomeningeal glioma (PLG) on the left parietal lobe of a 74-year-old woman and compared the tissue with heterotopic glial tissue from another case. The PLG tumor consisted of spindle-shaped cells with marked nuclear atypism, which tended to be arranged in a fascicular pattern, and the ma...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00310033
更新日期：1992-01-01 00:00:00
abstract：:A model of hypothermic circulatory arrest with recovery has been developed in the newborn dog. Eleven puppies were anesthetized with halothane, paralyzed and artificially ventilated with 70% nitrous oxide -30% oxygen to paO2 > 60 mm Hg, paCO2 = 33-42 mm Hg and pHa = 7.35-7.42. Animals were surface cooled to 20 degrees...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00227767
更新日期：1993-01-01 00:00:00
abstract：:The E200K mutation is the most frequent prion protein gene (PRNP) mutation detected worldwide that is associated with Creutzfeldt-Jakob disease (CJD) and thought to have overlapping features with sporadic CJD, yet detailed neuropathological studies have not been reported. In addition to the prion protein, deposition o...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/s00401-010-0713-y
更新日期：2011-01-01 00:00:00
abstract：:An 18 month old cat had a 6 month history of abnormal high stepping gait, ataxia, muscle twitching and loss of sensory perception. The clinical course was interrupted by several episodes of temporary remission. The essential pathological features were those of a polyradiculoneuritis with segmental demyelination, focal...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00689561
更新日期：1978-01-19 00:00:00
abstract：:Immunohistochemical analysis of inflammatory cell density and infiltrate subpopulations in 42 meningiomas was performed. Evaluation of infiltrating cell density was carried out by cell counting. Meningothelial and fibroblastic meningiomas contained an average of 3% mononuclear cells; the few lymphocytes were localized...
journal_title：Acta neuropathologica
pub_type： 杂志文章
doi：10.1007/BF00294381
更新日期：1989-01-01 00:00:00
