Abstract：
:Heat shock protein 90 (Hsp90) serves to stabilise and correctly fold multiple significant client proteins associated with cell proliferation and cell survival. However, little is known about the Hsp90 client proteins that regulate cell metabolism. Here, we describe a unique ability of Hsp90 to regulate the stability and activity of AMP-activated kinase (AMPK), a key sensor of cellular energy status. Hsp90 is found to interact with AMPK and to maintain its AMP-activated kinase activity, which in turn is required for the phosphorylation of its substrate, acetyl-CoA carboxylase (ACC), the key enzyme in fatty acid metabolism. Our binding analysis reveals that both the γ subunit and the α subunit of AMPK bind to Hsp90 with a high affinity. We demonstrate that Hsp90 inhibitors, including geldanamycin (GA) and mycoepoxydiene (MED), can induce the dissociation of AMPK from Hsp90, and cause a significant decrease in phosphorylation of AMPK and ACC. Furthermore, we demonstrate that shRNAs of Hsp90 can efficiently suppress the activation of AMPK. These findings not only establish a novel interaction between Hsp90 and AMPK but also suggest a new mechanism for regulating tumour cell fatty acid metabolism.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Zhang L,Yi Y,Guo Q,Sun Y,Ma S,Xiao S,Geng J,Zheng Z,Song Sdoi
10.1016/j.cellsig.2011.12.001subject
Has Abstractpub_date
2012-04-01 00:00:00pages
859-65issue
4eissn
0898-6568issn
1873-3913pii
S0898-6568(11)00379-2journal_volume
24pub_type
杂志文章abstract：:Homer1 protein is an important scaffold protein at postsynaptic density and has been demonstrated to play a central role in calcium signaling in the central nervous system. The aim of this study was to investigate the effects of Homer1 knockdown on MPP(+) induced neuronal injury in cultured dopamine (DA) neurons. We f...
journal_title：Cellular signalling
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更新日期：2013-12-01 00:00:00
abstract：:Protein kinase C and the annexins appear to share some unusual and potentially important membrane- and calcium-binding properties. While these proteins are calcium response elements, they are not calcium-binding proteins in the formal sense; at intracellular calcium concentrations, they only bind significant amounts o...
journal_title：Cellular signalling
pub_type： 杂志文章,评审
doi：10.1016/0898-6568(93)90075-w
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abstract：:Inflammation is characterized by early influx of polymorphonuclear neutrophils (PMNs), followed by a second wave of monocyte recruitment. PMNs mediate monocyte recruitment via their release of heparin binding protein (HBP), which activates CCR2 (CC-chemokine receptor 2) on monocytes. However, the pathways for such sig...
journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2017.12.008
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2016.04.012
更新日期：2016-08-01 00:00:00
abstract：:Protein kinase CK2 is a critical factor for the survival of cells. It is overexpressed in many cancer cells and provides protection against apoptosis in these cells. Inhibition of CK2 kinase activity in various cancer cells leads to apoptosis, which makes CK2 an attractive target for cancer therapy. Little is, however...
journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2014.03.014
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journal_title：Cellular signalling
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journal_title：Cellular signalling
pub_type： 杂志文章,评审
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journal_title：Cellular signalling
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doi：10.1016/0898-6568(94)00041-7
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2013.09.014
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journal_title：Cellular signalling
pub_type： 杂志文章
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更新日期：1989-01-01 00:00:00
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2018.09.016
更新日期：2019-01-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2012.07.014
更新日期：2012-11-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/0898-6568(95)00039-r
更新日期：1995-09-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/0898-6568(92)90023-2
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2010.04.008
更新日期：2010-09-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2010.10.011
更新日期：2011-02-01 00:00:00
abstract：:The focal adhesion kinase Pyk2 integrates signals from cell adhesion receptors, growth factor receptors, and G-protein-coupled receptors leading to the activation of intracellular signaling pathways that regulate cellular phenotypes. The intrinsic mechanism for the activation of Pyk2 activity remains to be fully defin...
journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2010.09.015
更新日期：2011-01-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章,评审
doi：10.1016/j.cellsig.2004.05.014
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/0898-6568(95)00019-l
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2010.10.017
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journal_title：Cellular signalling
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2015.07.010
更新日期：2015-10-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2006.12.005
更新日期：2007-06-01 00:00:00
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2015.02.028
更新日期：2015-06-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2013.11.005
更新日期：2014-02-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2015.01.003
更新日期：2015-04-01 00:00:00
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2005.09.009
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journal_title：Cellular signalling
pub_type： 杂志文章,评审
doi：10.1016/j.cellsig.2018.07.012
更新日期：2018-11-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2013.06.005
更新日期：2013-10-01 00:00:00
abstract：:C5L2 is a receptor that binds to C5a and belongs to the family of G protein-coupled receptors, but its role in physiological C5a-mediated responses remains under debate. Here we show that, like the canonical C5a receptor C5aR, C5L2 plays a pro-inflammatory role in a murine model of acute experimental colitis. We demon...
journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2014.02.021
更新日期：2014-07-01 00:00:00
