Abstract:
:Two samples of highly pure multiwalled carbon nanotubes (MWCNTs) similar in hydrophobicity and surface reactivity were prepared with similar length, <5 μm, but markedly different diameter (9.4 vs 70 nm). The samples were compared for their cytotoxic activity, uptake, and ability to induce oxidative stress (ROS production and intracellular GSH depletion) in vitro in murine alveolar macrophages (MH-S). The in vivo toxicity was evaluated by measuring biochemical (LDH activity and total proteins) and cellular responses in bronchoalveolar lavage (BAL) after intratracheal instillation in rats. Both samples were internalized in MH-S cells. However, thin MWCNTs appeared significantly more toxic than the thicker ones, both in vitro and in vivo, when compared on a mass-dose basis. The data reported herein suggest that the nanotube diameter is an important parameter to be considered in the toxicological assessment of CNTs.
journal_name
Chem Res Toxicoljournal_title
Chemical research in toxicologyauthors
Fenoglio I,Aldieri E,Gazzano E,Cesano F,Colonna M,Scarano D,Mazzucco G,Attanasio A,Yakoub Y,Lison D,Fubini Bdoi
10.1021/tx200255hsubject
Has Abstractpub_date
2012-01-13 00:00:00pages
74-82issue
1eissn
0893-228Xissn
1520-5010journal_volume
25pub_type
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