Abstract:
:A pair of chiral binuclear ruthenium(II) complexes were prepared and their binding affinities towards double stranded native DNA were assessed by observing isotropic absorption, polarized light spectra - circular and linear dichroism (CD and LD), fluorescence quenching and DNA thermal denaturation. Upon binding to DNA, the complexes produced LD signals consisting of positive and negative signals in the absorption region, although they exhibited red shift and hypochromism in the absorption spectrum. These contrasting observations indicated that the binding modes of the complexes are largely deviated from classical intercalative binding. Groove binding of the complexes to DNA was found to be more likely than intercalative binding. The small increase of DNA melting temperature in the presence of the complexes indicated a predominance of DNA groove binding. The absence of "molecular light switch effect" further supported non-intercalative binding. The groove binding propensity of complexes was also supported by comparison of the resulting data with the [Ru(phen)(2)(dppz)](2+).
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
Chitrapriya N,Jang YJ,Kim SK,Lee Hdoi
10.1016/j.jinorgbio.2011.08.016subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1569-75issue
12eissn
0162-0134issn
1873-3344pii
S0162-0134(11)00229-7journal_volume
105pub_type
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