Abstract:
:The CYP121 gene from the pathogenic bacterium Mycobacterium tuberculosis has been cloned and expressed in Escherichia coli, and the protein purified to homogeneity by ion exchange and hydrophobic interaction chromatography. The CYP121 gene encodes a cytochrome P450 enzyme (CYP121) that displays typical electronic absorption features for a member of this superfamily of hemoproteins (major Soret absorption band at 416.5 nm with alpha and beta bands at 565 and 538 nm, respectively, in the oxidized form) and which binds carbon monoxide to give the characteristic Soret band shift to 448 nm. Resonance Raman, EPR and MCD spectra show the protein to be predominantly low-spin and to have a typical cysteinate- and water-ligated b-type heme iron. CD spectra in the far UV region describe a mainly alpha helical conformation, but the visible CD spectrum shows a band of positive sign in the Soret region, distinct from spectra for other P450s recognized thus far. CYP121 binds very tightly to a range of azole antifungal drugs (e.g. clotrimazole, miconazole), suggesting that it may represent a novel target for these antibiotics in the M. tuberculosis pathogen.
journal_name
J Inorg Biochemjournal_title
Journal of inorganic biochemistryauthors
McLean KJ,Cheesman MR,Rivers SL,Richmond A,Leys D,Chapman SK,Reid GA,Price NC,Kelly SM,Clarkson J,Smith WE,Munro AWdoi
10.1016/s0162-0134(02)00479-8subject
Has Abstractpub_date
2002-09-20 00:00:00pages
527-41issue
4eissn
0162-0134issn
1873-3344pii
S0162013402004798journal_volume
91pub_type
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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journal_title:Journal of inorganic biochemistry
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pub_type: 杂志文章
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