Abstract:
:Larval locomotion is a sensitive readout of a range of nervous system deficits in Drosophila, and has been utilised to quantify modulation of the disease phenotype in models of human disease. Single larvae are typically analysed in series using manual quantification of parameters such as contraction rate, or grouped together and studied en-masse. Here, we describe the development of tests for the analysis of several spatially isolated third instar larvae in parallel. We rapidly quantify larval turning rate and velocity during wandering behaviour in a 4 plate assay. In a second test, larvae are recorded as they race along five parallel lanes towards a yeast stimulus. This allows increased throughput analysis of comparative genotypes simultaneously, video archiving, and detection of exacerbation or rescue of defective locomotion in a Drosophila model of tauopathy, as we demonstrate genetically and through delivery of candidate therapeutic chemicals in fly food. The tests are well-suited for rapid comparison of locomotion capability in Drosophila mutants or candidate modulation screens in Drosophila models of human disease.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Sinadinos C,Cowan CM,Wyttenbach A,Mudher Adoi
10.1016/j.jneumeth.2011.08.037subject
Has Abstractpub_date
2012-01-30 00:00:00pages
325-34issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(11)00496-1journal_volume
203pub_type
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