Abstract:
:O6-Methylguanine-DNA methyltransferase (MT) specific activity (fmol/mg protein) was measured in human T lymphocytes which were maintained as exponentially growing cultures from 6 to 17 days. The T lymphocytes were not transformed and were grown under the same conditions used previously for determination of spontaneous human mutant frequencies. Although large inter-individual differences in activity were found, the differences were not attributable to donor age, sex or time in culture. The reported specific activity results, including the age and sex independence, were similar to other laboratories even though non-cultured peripheral blood T lymphocytes were previously used. Since cells from Alzheimer's disease (AD) patients have been shown to be overly sensitive to alkylation damage induced by N-methyl-N'-nitro-N-nitrosoguanine, and since no one has previously assayed MT activity in cells from AD patients, we compared MT activities in cultured T lymphocytes from AD patients, healthy controls and neurological controls. Similar levels of MT specific activity were found in each category analysed.
journal_name
Mutagenesisjournal_title
Mutagenesisauthors
Bartlett JD,Robison SHdoi
10.1093/mutage/5.2.169subject
Has Abstractpub_date
1990-03-01 00:00:00pages
169-72issue
2eissn
0267-8357issn
1464-3804journal_volume
5pub_type
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