Abstract:
OBJECTIVE:replication of HIV-1 after cell entry is essentially dependent on the reverse transcriptase (RT). Antiretroviral drugs impairing the function of the RT currently aim at the polymerase subunit. One reason for failure of antiretroviral treatment is the evolvement of resistance-associated mutations in the viral genome. For RT inhibitors, almost all identified mutations are located within the polymerase; therefore, general genotyping confines to investigate this subunit. Recently several studies have shown that substitutions within the RNase H and the connection domain increase antiviral drug-resistance in vitro, and some of them are present in patient isolates. AIM:the aim of the present study was to investigate the prevalence of these substitutions and their association with mutations in the polymerase domain arising during antiretroviral treatment. MATERIAL AND METHODS:we performed genotypic analyzes on seventy-four virus isolates derived from treated and untreated patients, followed at the HIV Centre of the Johann Wolfgang Goethe University Hospital (Frankfurt/Main, Germany). We subsequently ana?lysed the different substitutions in the c-terminal region to evaluate whether there were associations with each other, n-terminal substitutions or with antiretroviral treatment. RESULTS:We identified several primer grip substitutions, but almost all of them were located in the connection domain. This is consistent with other in-vivo studies, in which especially the primer grip residues located in the RNase H were unvaried. Furthermore, we identified other substitutions in the connection domain and in the RNase H. Especially E399D seemed to be associated with an antiretroviral treatment and N-terminal resistance-delivering mutations. CONCLUSION:some of the identified substitutions were associated with antiviral treatment and drug resistance-associated mutations. Due to the low prevalence of C-terminal mutations and as only a few of them could be associated with antiviral treatment and N-terminal resistance-delivering mutations, we would not recommend routinely testing of the C-terminal RT region.
journal_name
Eur J Med Resjournal_title
European journal of medical researchauthors
Michels I,Staszewski S,Gürtler L,Nisius G,Müller A,Locher L,Doerr HW,Stürmer Mdoi
10.1186/2047-783x-15-10-415subject
Has Abstractpub_date
2010-10-25 00:00:00pages
415-21issue
10eissn
0949-2321issn
2047-783Xjournal_volume
15pub_type
杂志文章abstract::CMV infection is common in pediatric HIV-1 infected patients. We present a case report, to our knowledge the first pediatric patient, in which Addison-disease due to CMV was suspected during lifetime. We want to point out the importance of routine clinical and laboratory follow up once a HIV-1 infected child is shown ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-06-20 00:00:00
abstract:OBJECTIVE:Evaluating the effects of prophylactic administration of IgM-enriched immunoglobulins (IVIG) on immunological- and clinical parameters in cardiac surgical patients. PATIENTS AND METHODS:41 patients were randomized to receive either an IgM-enriched immunoglobulin (Pentaglobin(R)) preparation (1,300 ml immunog...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-12-17 00:00:00
abstract:BACKGROUND:Osteoporosis affects 200 million people worldwide and places an enormous economic burden on society. We aim to identify the feature genes that are related to osteoprotegerin in osteoporosis and to perform function analysis with DNA microarray from human bone marrow. METHODS:We downloaded the gene expression...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-15
更新日期:2013-06-03 00:00:00
abstract:BACKGROUND:It is hitherto still questionable which of the physical properties of the bile fluid influence the outcome of extracorporeal shock wave lithotripsy (ESWL). METHODS:In this study the influence of the density and viscosity of bile simulating fluid on ESWL was tested in vitro by applying CsCl solutions of dens...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1996-01-19 00:00:00
abstract:BACKGROUND:Children with persistent mild to moderate bronchial asthma require anti-inflammatory therapy. According to current treatment guidelines both sodium cromoglycate (SCG) and inhaled corticosteroids can be used. If children remain symptomatic despite regular SCG therapy, corticosteroids are the next therapeutic ...
journal_title:European journal of medical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2002-06-28 00:00:00
abstract:BACKGROUND:Drug-eluting stents (DES) compared to bare metal stents (BMS) have shown superior clinical performance, but are considered less suitable in complex cases. Most studies do not distinguish between DES and BMS with respect to their mechanical performance. The objective was to obtain mechanical parameters for di...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-017-0300-y
更新日期:2018-01-08 00:00:00
abstract::Cancer and cardiovascular diseases are the main causes for morbidity and mortality in modern society. In the United States of America (USA), over 1.7 million new cancer cases will presumably be observed in 2018. Progress in cancer treatment has greatly improved survival and it is estimated that 15.5 million cancer sur...
journal_title:European journal of medical research
pub_type: 社论
doi:10.1186/s40001-018-0359-0
更新日期:2019-01-03 00:00:00
abstract::Basal cell carcinoma (BCC) is the most common skin cancer, occurring more frequently than malignancies of any other tissue or organ, either individually or in total. Medical treatment modalities of BCC offer cost reduction and clinical advantages in selected cases. Neomycin has been reported to have an important role ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2005-05-20 00:00:00
abstract::The aim of the study was to investigate whether cholecystokinin, neurotensin, and cholinergic mechanisms act as mediators of bile salt-stimulated exocrine pancreatic secretion. Ten fasting healthy subjects provided with a double-lumen tube received 2, 4, and 6 g cattle bile and 200, 400, and 600 mg Na-taurodeoxycholat...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:Patients with a simple transversal fracture of the olecranon are often treated with a tension band wiring (TBW), because it is known as a biomechanically appropriate and cost-effective procedure. Nevertheless, the technique is in detail more challenging than thought, resulting in a considerable high rate of ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-019-0392-7
更新日期:2019-10-08 00:00:00
abstract::A number of neurodegenerative diseases including Parkinson's disease (PD) have been shown to be associated with polymorphic variants of mitochondrial DNA. The A4336G mutation in the tRNA(Gln) gene was reported to occur at a higher frequency in individuals with Parkinson's disease and Alzheimer's disease (AD) than in a...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1997-03-24 00:00:00
abstract::Progressive infantile spastic tetraparesis spans a wide spectrum of partially rare differential diagnoses. Based on a clinical example the differential diagnostic thoughts are discussed in detail. Though juvenile motor neuron disease is a rare entity, it has to be kept in mind for differential diagnostics in cases of ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2008-10-27 00:00:00
abstract:OBJECTIVES:The present study was carried out to assess compliance rates with annual colorectal cancer screening for fecal occult blood, and to identify the subjects with higher priority in health education to increase screen compliance. DESIGN AND SETTING:A screening program for colorectal cancer was conducted between...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:1998-07-20 00:00:00
abstract::The purpose of this report is to document a case of unsuspected ameloblastoma involving the right man dibular subpontic region in a 38-year-old Cambodian female patient. This lesion was purportedly preceded by multiple radiolucencies which were diagnosed as radicular cysts and treated a few times in the past years by ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-15-3-135
更新日期:2010-03-30 00:00:00
abstract::We report the case of a 64-year old woman with severe attacks of allergic alveolitis occurring frequently independent of a concrete place. These attacks were regularely preceded by diarrhoe. Laboratory data showed marked leukopenia and circulating Candida albicans (C.a.)-antigen. Bronchoalveolar lavage revealed an inc...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-03-27 00:00:00
abstract:BACKGROUND:The literature did not show clearly if a correlation between trunk and facial asymmetry exists. The aim of this study was to verify the association between trunk and facial asymmetries, and trunk and facial sagittal configuration in adolescents. METHODS:This is a cross-sectional screening study. It was carr...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-017-0280-y
更新日期:2017-09-29 00:00:00
abstract:BACKGROUND:Exacerbations of chronic obstructive pulmonary disease (COPD) are sporadic, acute worsening of symptoms. Identifying predictors of exacerbation frequency may facilitate medical interventions that reduce exacerbation frequency and severity. The objective of this study was to determine predictors of exacerbati...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-19-18
更新日期:2014-04-08 00:00:00
abstract::Pentalogy of Fallot is a rare cyanotic congenital heart disease characterized by biventricular origin of the aorta above a large ventricular septal defect, obstruction of the pulmonary outflow, right ventricular hypertrophy (tetralogy of Fallot), and an atrial septal defect. Mortality due to syncope, arrhythmia or con...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2001-06-28 00:00:00
abstract:BACKGROUND:Hereditary non-polyposis colorectal cancer (HNPCC) is an autosomal dominant tumor syndrome predisposing to predominantly colorectal and endometrial cancer. In 90% of the cases, molecular analyses reveal microsatellite instabilities due to germline mutations in DNA mismatch repair (MMR) genes, mainly MLH1, MS...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2005-01-28 00:00:00
abstract:BACKGROUND:Prostate cancer (PCa) originating from the prostate base may intrude into the urinary bladder and may be misdiagnosed as bladder cancer. In this retrospective study, we reviewed the clinic data on PCa cases which were initially misdiagnosed as bladder cancer in order to identify diagnostic methods that would...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-36
更新日期:2013-10-03 00:00:00
abstract:BACKGROUND:Fingertip injuries treated with occlusive dressings (ODs) lead to nearly scar-free, functionally, and aesthetically pleasing results. We hypothesized that paracrine factors in the wound fluid (secretome) may influence migration and proliferation of mesenchymal stem cells (MSCs) and fibroblasts and modulate t...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-018-0357-2
更新日期:2018-12-26 00:00:00
abstract:BACKGROUND:Dopamine agonists (DA) are the first-choice drug for treatment of the early stage of Parkinson's disease (PD) in subjects younger than 70 years. Recently, a number of third generation DA have been marketed, including transdermal patch of rotigotine and extended release oral formulation of ropinirole and pram...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-60
更新日期:2013-12-21 00:00:00
abstract:BACKGROUND:The immunological and clinical benefits of structured treatment interruptions (STIs) during primary HIV-1 infection remain largely unclear. PATIENTS AND METHODS:Eight patients identified during primary HIV-1 infection were immediately treated with HAART and underwent subsequent STIs after reaching complete ...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2006-07-31 00:00:00
abstract::The Gasperini syndrome is a very rare brainstem disease characterized by the typical combination of ipsilateral lesions of the cranial nerves V-VII and dissociated contralateral hemihypesthesia, whereas both contralateral and ipsilateral hypacusis was described. Since the first description in 1912, only a few cases of...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-9-413
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:The study investigated the feasibility and potential outcome measures during acute hyperoxia in type 2 diabetes patients (DM2). METHODS:Eleven DM2 patients (7 men and 4 women) were included in the study. The patients cycled (30 min at 20% Wmax) whilst breathing three different supplemental oxygen flows (SOF,...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/s40001-015-0194-5
更新日期:2016-01-08 00:00:00
abstract:BACKGROUND:The aim of the study was to test for human papilloma virus (HPV) infection and human telomerase RNA component (hTERC) gene amplification in tissues derived from esophageal cancer, in esophagus displaying atypical hyperplasia and in normal tissue, and to analyze the relationship between them and discuss wheth...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783X-18-11
更新日期:2013-05-01 00:00:00
abstract::Under antidepressive treatment with amitryptiline (100 mg/d) a 71-year old woman developed delirious symptoms, hyponatremia and a grand mal seizure followed by cardiovascular arrest. A few month later she ingested 48 mg reboxetine with suicidal intent. Overdosing of reboxetine, a selective noradrenaline re-uptake inhi...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2002-09-30 00:00:00
abstract:BACKGROUND:Vascular injury is the main mechanism in pathophysiology of PR3-ANCA-associated vasculitis. Soluble serum thrombomodulin (sTM) is a membrane-bound receptor for thrombin expressed by vascular endothelial cells. - OBJECTIVE:The aim of study was to determine the blood levels of sTM in patients with PR3-ANCA-as...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:10.1186/2047-783x-14-s4-268
更新日期:2009-12-07 00:00:00
abstract::Fluid shift after entering into microgravity, but also under equivalent flight conditions leads to enormous increase of intraocular pressure. To assess this precisely position - and gravity independent, handsome, automatic tonometers have been developed (German-Spacelab D1-Mission, German-Spacelab D2-Mission, German-R...
journal_title:European journal of medical research
pub_type: 杂志文章
doi:
更新日期:2000-01-26 00:00:00
abstract::In 1973 copper-associated liver disease was first described in an Australian boy of non-Indian origin who died at the age of 15 months with liver cirrhosis. At necropsy the hepatic copper concentration was excessively high (> 300 mg/g dry weight). It was shown that the boy s disease was caused by consumption of copper...
journal_title:European journal of medical research
pub_type: 历史文章,杂志文章
doi:
更新日期:1999-06-28 00:00:00