当前位置： SCI文献检索 > ANNALS OF NUCLEAR MEDICINE期刊下所有文献 > Comparison of (99m)Tc-labeled PR81 and its F(ab')₂ fragments as radioimmunoscintigraphy agents for breast cancer imaging.

Comparison of (99m)Tc-labeled PR81 and its F(ab')₂ fragments as radioimmunoscintigraphy agents for breast cancer imaging.

Abstract：

OBJECTIVE:We digested anti-MUC1 monoclonal antibody PR81 to produce F(ab')₂ fragments. A comparison was performed between the two radiolabeled PR81 and F(ab')₂ fragments for breast tumor imaging in a mouse model. METHODS:The optimum conditions for pepsin digestion of PR81 were investigated in terms of enzymes: antibody ratio, digestion time duration and preserved immunoreactivity of the produced fragments. The F(ab')₂ fragments were labeled with Technetium-99m using HYNIC as a chelator and tricine as a co-ligand. The immunoreactivity of the complexes was assessed by radioimmunoassay using MCF7 cells. Biodistribution and imaging studies were performed in female BALB/c mice with breast tumor xenograft at 4, 8 and 24 h post-administration. The PR81 was labeled with technetium-99m in the same way for comparison. RESULTS:The optimum time duration for PR81 digestion was found to be 28 h at an enzyme:antibody weight ratio of 1:20 that resulted in 95.2 ± 4.7% purity. The labeling of intact PR81 and its F(ab')₂ fragments were 87.6 ± 4.2 and 76.1 ± 3.3% after 1 h, respectively (p value <0.05). The percentage of immunoreactivity of F(ab')₂ fragments and intact PR81 were 75.4 ± 2.1% and 85.7 ± 2.9%, respectively (p value <0.05). The biodistribution and imaging studies demonstrated localization of the fragments at 4 h post-administration with high sensitivity and specificity. CONCLUSION:The results showed that F(ab')₂ fragment of PR81 is more suitable than intact PR81 for safer and more rapid detection of human breast cancer.

journal_name

Ann Nucl Med

journal_title

Annals of nuclear medicine

authors

Salouti M,Babaei MH,Rajabi H,Foroutan H,Rasaee MJ,Rajabi AB,Mohammadnejad J,Shafiee M,Mazidi M,Daha FJ

doi

10.1007/s12149-010-0434-2

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

87-92

issue

2

eissn

0914-7187

issn

1864-6433

journal_volume

25

pub_type

杂志文章

相关文献

ANNALS OF NUCLEAR MEDICINE文献大全
  • Washout rates of Tc-99m tetrofosmin in asymmetric septal hypertrophy.

    abstract：:The aim of this study was to evaluate the washout rate of Tc-99m tetrofosmin in asymmetric septal hypertrophy (ASH). As Tc-99m tetrofosmin accumulation and retention depend on sarcolemmal and/or mitochondrial function, the presence or absence of abnormalities in the washout rate of Tc-99m tetrofosmin could provide inf...

    journal_title：Annals of nuclear medicine

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF02986332

    authors： Büyükdereli G,Kanadasi M,Kibar M

    更新日期：2005-02-01 00:00:00

  • Synthesis and evaluation of radioiodinated phenoxyquinazoline and benzylaminoquinazoline derivatives as new EGF receptor tyrosine kinase imaging ligands for tumor diagnosis using SPECT.

    abstract：OBJECTIVE:Epidermal growth factor receptor tyrosine kinase (EGFR-TK) represents an attractive target for tumor diagnosis agents. Previously, the radioiodinated 4-(3-iodoanilino)-6,7-diethoxyquinazoline ([(125)I]m-IPQ) has been reported to possess good characteristics as a tumor imaging agent; however, it was also found...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-012-0583-6

    authors： Hirata M,Kanai Y,Naka S,Matsumuro K,Kagawa S,Yoshimoto M,Ohmomo Y

    更新日期：2012-06-01 00:00:00

  • Visual and semi-quantitative analyses of dual-phase breast-specific gamma imaging with Tc-99m-sestamibi in detecting primary breast cancer.

    abstract：OBJECTIVES:Breast cancer is the most common malignancy for females worldwide. This study was to evaluate the application of dual-phase breast-specific gamma imaging (BSGI) in detecting primary breast cancer. METHODS:Seventy-six patients with indeterminate breast lesions that underwent dual-phase BSGI enrolled in this ...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-013-0776-7

    authors： Tan H,Jiang L,Gu Y,Xiu Y,Han L,Wu P,Zhang H,Shi H

    更新日期：2014-01-01 00:00:00

  • Assessment of collimators in radium-223 imaging with channelized Hotelling observer: a simulation study.

    abstract：OBJECTIVE:Radium-223 (223Ra) is used in unsealed radionuclide therapy for metastatic bone tumors. The aim of this study is to apply a computational model observer to 223Ra planar images, and to assess the performance of collimators in 223Ra imaging. METHODS:The 223Ra planar images were created via an in-house Monte Ca...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-018-1286-4

    authors： Takahashi A,Baba S,Sasaki M

    更新日期：2018-12-01 00:00:00

  • Distinguishing benign from malignant gallbladder wall thickening using FDG-PET.

    abstract：OBJECTIVE:Because thickening of the gallbladder wall is observed not only in patients with gallbladder cancer but also in those with benign diseases such as chronic cholecystitis and gallbladder adenomyosis, it is difficult to distinguish between benign and malignant gallbladder wall thickening by conventional techniqu...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02984683

    authors： Oe A,Kawabe J,Torii K,Kawamura E,Higashiyama S,Kotani J,Hayashi T,Kurooka H,Tsumoto C,Kubo S,Shiomi S

    更新日期：2006-12-01 00:00:00

  • Clinical significance of diastolic function as an indicator of myocardial ischemia assessed by 16-frame gated myocardial perfusion SPECT.

    abstract：OBJECTIVE:Studies have suggested that ischemia-induced diastolic dysfunction persists longer than systolic dysfunction. We examined whether global left ventricular (LV) diastolic function during stress testing assessed by 16-frame gated myocardial perfusion single-photon emission computed tomography (SPECT) is useful a...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-008-0174-8

    authors： Nakae I,Matsuo S,Matsumoto T,Mitsunami K,Horie M

    更新日期：2008-10-01 00:00:00

  • Scintigraphic changes in bone metastasis from prostate cancer after hormonal therapy--comparison with tumor markers and bone X-ray.

    abstract：:Bone scintigraphy is often performed to assess the response to systemic therapy of bone metastasis from prostate cancer. We examined the changes in bone scintigraphic findings and the agreement with AIP, AcP, or other tumor markers measured in the follow-up of patients with known bone metastasis after hormonal therapy...

    journal_title：Annals of nuclear medicine

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF03165024

    authors： Koizumi K,Uchiyama G,Komatsu H

    更新日期：1994-11-01 00:00:00

  • [99mTc]3PRGD2 for integrin receptor imaging of esophageal cancer: a comparative study with [18F]FDG PET/CT.

    abstract：OBJECTIVES:This study was designed to investigate the efficacy of 99mtechnetium-three polyethylene glycol spacers-arginine-glycine-aspartic acid ([99mTc]3PRGD2) in the evaluation of patients with esophageal cancer. METHODS:Twenty-nine patients with a suspected esophageal lesion and for whom definite pathological diagn...

    journal_title：Annals of nuclear medicine

    pub_type： 临床试验,杂志文章

    doi：10.1007/s12149-018-1315-3

    authors： Zheng S,Chen Z,Huang C,Chen Y,Miao W

    更新日期：2019-02-01 00:00:00

  • Relationship between bone scintigraphy and tumor markers in patients with breast cancer.

    abstract：PURPOSE:The aim of this study is to specify the precise role of bone scintigraphy and serum CEA and CA 15-3 assays in the monitoring of breast cancers in order to optimize their use and to determine whether it is possible to guide the prescription of bone scan by the use of CEA and CA 15-3 assays in the monitoring of b...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02984567

    authors： Yildiz M,Oral B,Bozkurt M,Cobaner A

    更新日期：2004-09-01 00:00:00

  • Evaluation of pleural lesions after pleurodesis with OK-432 by fluorodeoxyglucose-positron emission tomography/CT.

    abstract：OBJECTIVE:This study aimed to determine changes in FDG-PET/CT after pleurodesis with OK-432 and to investigate differences in the changes between non-malignant and malignant lesions. METHODS:Study participants were 17 patients with a history of malignant chest disease who underwent FDG-PET/CT after pleurodesis using O...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-020-01508-0

    authors： Nishimori M,Yoshimatsu R,Iwasa H,Miyatake K,Nitta N,Anayama T,Yamagami T

    更新日期：2020-10-01 00:00:00

  • Unusual patterns of I-131 contamination.

    abstract：:Whole body imaging with radioiodine can detect functioning metastases, which can often be effectively treated with appropriate amounts of radioiodine. Non-physiologic I-131 uptake detected on images is usually interpreted as suggesting functioning thyroid metastases. However, extra-thyroidal I-131 accumulation does no...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02985010

    authors： Ozguven M,Ilgan S,Arslan N,Karacalioglu AO,Yuksel D,Dundar S

    更新日期：2004-05-01 00:00:00

  • Uptake and washout of I-123-MIBG in neuronal and non-neuronal sites in rat hearts: relationship to renal clearance.

    abstract：:We investigated the uptake and washout of I-123-metaiodobenzylguanidine (MIBG) in neuronal (both intra- and extravesicular) and non-neuronal sites in the heart and its relationship to renal clearance. Acute renal failure was induced in rats by ligating the renal vessels, and the findings were compared with those of sh...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF03165394

    authors： Arbab AS,Koizumi K,Araki T

    更新日期：1996-05-01 00:00:00

  • Lack of correlation between Tc-99m-sestaMIBI uptake and cadherin expression in infiltrating ductal breast carcinoma as prognostic indicators.

    abstract：:Despite using various kinds of prognostic indicators, it is still not possible to predict the biological behavior of breast cancer in all patients. Tc-99m-sestaMIBI (MIBI) uptake determined by breast scintigraphy and cadherin expression of tumor tissue revealed by immunohistochemistry are suggested as potential agents...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02988522

    authors： Uğur Y,Sari O,Uğur O,Korkusuz P,Varoğlu E,Arslan N,Gürcan N,Yildirim M,Sökmensüer C,Aşan E,Aras T

    更新日期：2003-06-01 00:00:00

  • 125I-iomazenil-benzodiazepine receptor binding during psychological stress in rats.

    abstract：OBJECTIVE:We investigated the changes in 125I-iomazenil (125I-IMZ) benzodiazepine receptor (BZR) binding with psychological stress in a rat model. METHODS:Six male Wistar rats were placed under psychological stress for 1 hour by using a communication box. No physical stress was not received. 1.85 MBq of 125I-IMZ was i...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02996307

    authors： Fukumitsu N,Tsuchida D,Ogi S,Uchiyama M,Mori Y

    更新日期：2002-05-01 00:00:00

  • Does lung perfusion scintigraphy continue to have a role in the clinical management of patients suspected of pulmonary embolism in the CT pulmonary angiography era?

    abstract：OBJECTIVE:Acute pulmonary embolism (PE) is a life-threatening disorder with high mortality. A prompt diagnosis and treatment is essential for reducing the mortality rate. The purpose of the study is to evaluate if lung perfusion scintigraphy (LPS) continues to have a role in the clinical management of patients suspecte...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-018-1295-3

    authors： Koukouraki SI,Hatzidakis AA,Mitrouska I,Stathaki MI,Perisinakis K

    更新日期：2018-12-01 00:00:00

  • Performance profile of FDG-PET and PET/CT for cancer screening on the basis of a Japanese Nationwide Survey.

    abstract：OBJECTIVE:The aim of this study is to survey the situation of (18)F-fluorodeoxyglucose-positron emission tomography (FDG-PET) cancer screening in Japan and to describe its performance profile. METHODS:"FDG-PET for cancer screening" was defined as FDG-PET or positron emission tomography and computed tomography (PET/CT)...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章,多中心研究

    doi：10.1007/s12149-007-0061-8

    authors： Minamimoto R,Senda M,Uno K,Jinnouchi S,Iinuma T,Ito K,Okuyama C,Oguchi K,Kawamoto M,Suzuki Y,Tsukamoto E,Terauchi T,Nakashima R,Nishio M,Nishizawa S,Fukuda H,Yoshida T,Inoue T

    更新日期：2007-11-01 00:00:00

  • Receiver operating characteristic (ROC) analysis of images reconstructed with iterative expectation maximization algorithms.

    abstract：PURPOSE:The quality of images reconstructed by means of the maximum likelihood-expectation maximization (ML-EM) and ordered subset (OS)-EM algorithms, was examined with parameters such as the number of iterations and subsets, then compared with the quality of images reconstructed by the filtered back projection method....

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02988506

    authors： Takahashi Y,Murase K,Higashino H,Sogabe I,Sakamoto K

    更新日期：2001-12-01 00:00:00

  • Quantifying regional cerebral blood flow with N-isopropyl-p[123I]iodoamphetamine by ring-type single-photon emission computed tomography: validity of a method to estimate early reference value by means of regional brain time-activity curve.

    abstract：:A more accurate quantitative method for the measurement of regional cerebral blood flow (rCBF) with the microsphere model and N-isopropyl-p-[123I]iodoamphetamine (123I-IMP) and ring-type single-photon emission computed tomography (SPECT) was developed. Continuous withdrawal of arterial blood was carried out for 5 minu...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF03165028

    authors： Takahashi N

    更新日期：1994-11-01 00:00:00

  • The role of 13N-ammonia in the differential diagnosis of gliomas and brain inflammatory lesions.

    abstract：OBJECTIVE:To investigate the utility of 13N-ammonia PET/CT imaging in the differential diagnosis of gliomas and brain inflammations. METHODS:13N-ammonia PET/CT imaging data of 77 patients with gliomas and 34 patients with brain inflammations were retrospectively analyzed. No patients received any treatment before 13N-...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-018-1308-2

    authors： Yi C,Shi X,Zhang X,Luo G,Zhang B,Zhang X

    更新日期：2019-01-01 00:00:00

  • FDG-PET of patients with suspected renal failure: standardized uptake values in normal tissues.

    abstract：OBJECTIVE:This study aims to clarify the effect of renal function on 2-[(18)F] fluoro-2-deoxy-D-glucose positron emission tomography (FDG-PET) imaging and determine the clinical significance of renal function in this setting. We compared FDG distribution between normal volunteers and patients with suspected renal failu...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-007-0012-4

    authors： Minamimoto R,Takahashi N,Inoue T

    更新日期：2007-06-01 00:00:00

  • Myocardial accumulation of a dopamine D2 receptor-binding radioligand, 2'-iodospiperone.

    abstract：:125I-2'-iodospiperone (2'-ISP), which has a high and selective affinity for dopamine D2 receptors, produced a high myocardial accumulation of radioactivity in the early phase after intravenous injection into mice. A human scintigraphic study also showed that the myocardium was clearly visualized soon after intravenous...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF03164959

    authors： Saji H,Yonekura Y,Tanahashi K,Iida Y,Iwasaki Y,Magata Y,Konishi J,Yokoyama A

    更新日期：1993-08-01 00:00:00

  • Thallium-201 brain SPECT to diagnose aggressiveness of meningiomas.

    abstract：:This investigation was conducted to determine the ability of 201Tl brain SPECT with respect to preoperative prediction of lesional aggressiveness of meningioma. Fifty-nine lesions in 42 patients were examined. Early (15 min) and late (3 h) SPECT were obtained. Early uptake ratio (ER; lesion to normal brain average cou...

    journal_title：Annals of nuclear medicine

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF03006435

    authors： Kinuya K,Ohashi M,Itoh S,Sakai S,Yamamoto K,Kakuda K,Nobata K,Terahata S,Taki S,Kinuya S

    更新日期：2003-09-01 00:00:00

  • Morphine-augmented cholescintigraphy enhances duodenogastric reflux.

    abstract：:Morphine intervention in cholescintigraphy decreases imaging time to diagnose acute cholecystitis. Not infrequently we observe duodenogastric reflux during scintigraphy with and without morphine intervention. To evaluate occurrence of duodenogastric reflux related to morphine, we reviewed 55 patients who underwent cho...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF03168405

    authors： Shih WJ,Lee JK,Magoun S,Wierzbinski B,Ryo UY

    更新日期：1995-11-01 00:00:00

  • Value of analyzing deep gray matter and occipital lobe perfusion to differentiate dementia with Lewy bodies from Alzheimer's disease.

    abstract：OBJECTIVE:Dementia with Lewy bodies (DLB) is generally characterized by a decrease in regional cerebral blood flow (rCBF) in the occipital lobe. However, not all patients with DLB have this feature. We explored characteristics of rCBF pattern changes to improve the identification of DLB, in addition to occipital hypope...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-008-0193-5

    authors： Shimizu S,Hanyu H,Hirao K,Sato T,Iwamoto T,Koizumi K

    更新日期：2008-12-01 00:00:00

  • Development of a simple non-invasive microsphere quantification method for cerebral blood flow using I-123-IMP.

    abstract：OBJECTIVE:In clinical practice, measurement of the rCBF has mainly been conducted by I-123-N-isopropyl-p-iodoamphetamine ((123)I-IMP) SPECT using the microsphere (MS) method, with continuous arterial blood sampling. While several non-invasive (123)I-IMP quantification methods have been developed, their accuracy has bee...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-015-1053-8

    authors： Ofuji A,Mimura H,Yamashita K,Takaki A,Sone T,Ito S

    更新日期：2016-04-01 00:00:00

  • Comparison of FDG-PET, MRI and CT for post radiofrequency ablation evaluation of hepatic tumors.

    abstract：PURPOSE:Treatment effect of radiofrequency ablation (RFA) is traditionally accomplished with MRI and/or CT. The aim of the study was to assess the role of FDG-PET in post RFA hepatic tumor evaluation, in comparison with MRI and CT. MATERIALS AND METHODS:28 patients (33 hepatic RFA lesions) who had post RFA FDG-PET wit...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-012-0656-6

    authors： Chen W,Zhuang H,Cheng G,Torigian DA,Alavi A

    更新日期：2013-01-01 00:00:00

  • (99m)Tc-DTPA dynamic SPECT and CT volumetry for measuring split renal function in live kidney donors.

    abstract：OBJECTIVES:Split renal function (SRF) estimated from the posterior view of (99m)Tc-diethylenetriaminepentaacetic acid planar scintigraphy (DTPA/P) is not sufficiently accurate even after correction for kidney depth by computed tomography (CT). To obtain more accurate SRF using (99m)Tc-DTPA, dynamic SPECT method was car...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-010-0349-y

    authors： Miyazaki C,Harada H,Shuke N,Okizaki A,Miura M,Hirano T

    更新日期：2010-04-01 00:00:00

  • Novel algorithm for quantitative assessment of left ventricular dyssynchrony with ECG-gated myocardial perfusion SPECT: useful technique for management of cardiac resynchronization therapy.

    abstract：OBJECTIVES:Cardiac resynchronization therapy (CRT) is the established treatment for patients with chronic and severe heart failure, and it has been reported that the presence of left ventricular (LV) dyssynchrony is one of the most important factors which predict positive response of this therapy. In the present study,...

    journal_title：Annals of nuclear medicine

    pub_type： 临床试验,杂志文章

    doi：10.1007/s12149-011-0525-8

    authors： Kiso K,Imoto A,Nishimura Y,Kanzaki H,Noda T,Kamakura S,Ishida Y

    更新日期：2011-12-01 00:00:00

  • Tc-99m depreotide SPECT/CT for lymph node staging of non-small-cell lung cancer.

    abstract：OBJECTIVE:To investigate the potential role of Tc-99m depreotide (Tc-DEPR) in the preoperative lymph node (N) staging of non-small-cell lung cancer (NSCLC). METHODS:Sixty-one patients with NSCLC at the potentially operable stage were enrolled and underwent scintigraphy before surgery (n=56) or mediastinoscopy (n=5). I...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/s12149-014-0839-4

    authors： Apostolopoulos DJ,Koletsis EN,Spyridonidis T,Paschali A,Papandrianos N,Stamou E,Vassilakos PJ,Dougenis D

    更新日期：2014-06-01 00:00:00

  • Dilated cardiomyopathy relieved as a result of beta-blocker therapy: a case report--key points in assessment of prognosis based on MIBG myocardial scintigraphy and BNP levels.

    abstract：:A 48-year-old male patient was admitted to our hospital with dyspnea accompanied by orthopnea. Chest x-rays showed a cardiothoracic ratio of 68% and pulmonary congestion. He was diagnosed with dilated cardiomyopathy. Beta-Blocker (carvedilol) therapy was initiated on Day 22 of the disease using a small initial dose. H...

    journal_title：Annals of nuclear medicine

    pub_type： 杂志文章

    doi：10.1007/BF02984613

    authors： Matsuo S,Nakae I,Masuda D,Matsumoto T,Horie M

    更新日期：2005-05-01 00:00:00