Proposal of an in vivo comet assay using haemocytes of Drosophila melanogaster.

abstract：:In this study, the genotoxic effects of a widely used herbicide, trifluralin, and its commercial formulation, Treflan, were evaluated using the micronucleus test in a commercially important fish species, Oreochromis niloticus (Nile Tilapia). Fish were exposed to 1, 5, and 10 microg/L doses of trifluralin and Treflan f...

journal_title：Environmental and molecular mutagenesis

authors： Könen S,Cavaş T

更新日期：2008-07-01 00:00:00

abstract：:Ethylene oxide (EO) is a direct acting alkylating agent; in vitro and in vivo studies indicate that it is both a mutagen and a carcinogen. However, it remains unclear whether the mode of action (MOA) for cancer for EO is a mutagenic MOA, specifically via point mutation. To investigate the MOA for EO-induced mouse lung...

journal_title：Environmental and molecular mutagenesis

authors： Manjanatha MG,Shelton SD,Chen Y,Parsons BL,Myers MB,McKim KL,Gollapudi BB,Moore NP,Haber LT,Allen B,Moore MM

更新日期：2017-04-01 00:00:00

abstract：:A mutagenesis moonshot addressing the influence of the environment on our genetic wellbeing was launched just 2 months before astronauts landed on the moon. Its impetus included the discovery that X-rays (Muller HJ. [1927]: Science 64:84-87) and chemicals (Auerbach and Robson. [1946]: Nature 157:302) were germ-cell mu...

journal_title：Environmental and molecular mutagenesis

authors： DeMarini DM

更新日期：2020-01-01 00:00:00

abstract：:Benzo(a)pyrene (BaP) is a mutagenic carcinogen that is ubiquitous in our environment. To better understand the toxic effects of BaP and to explore the relationship between toxicity and toxicogenomics profiles, we assessed global mRNA and microRNA (miRNA) expression in Muta™Mouse. Adult male mice were exposed by oral g...

journal_title：Environmental and molecular mutagenesis

authors： Malik AI,Williams A,Lemieux CL,White PA,Yauk CL

更新日期：2012-01-01 00:00:00

abstract：:A study of meiotic and postmeiotic germ-cell-stage sensitivity of male mice to induction of unscheduled DNA synthesis (UDS) by acrylamide showed that DNA repair could be detected in early spermatocytes (after the last scheduled DNA synthesis) through about mid-spermatid stages. No DNA repair could be detected in later...

journal_title：Environmental and molecular mutagenesis

authors： Sega GA,Generoso EE,Brimer PA

更新日期：1990-01-01 00:00:00

abstract：:The influence of several methodological factors on mean values of sister chromatid exchanges (SCEs) and micronuclei (MN) in peripheral lymphocytes of 1,650 subjects was analyzed. Donors belonged to a general healthy population living in Pisa and in two nearby small cities: Cascina and Navacchio (Ca-Na). Blood samples ...

journal_title：Environmental and molecular mutagenesis

authors： Barale R,Marrazzini A,Bacci E,Di Sibio A,Tessa A,Cocchi L,Scarcelli V,Lubrano V,Vassalle C,Landi S

更新日期：1998-01-01 00:00:00

abstract：:Genetic polymorphisms have been reported in several cytochrome P450 (CYP) genes, including CYP1B1 which metabolically activates procarcinogens present in tobacco to carcinogenic intermediates. This study used a case-control approach in North Indian population to determine associations between genetic variants in CYP1B...

journal_title：Environmental and molecular mutagenesis

authors： Katiyar T,Maurya SS,Hasan F,Singh AP,Khan AJ,Hadi R,Singh S,Bhatt MLB,Parmar D

更新日期：2017-07-01 00:00:00

abstract：:The effects of ultrasound on the cytoskeleton, comprising microtubules, had been studied decades ago. Nonetheless, very little attention has been paid to the effects of ultrasound on the mitotic spindle, which is also formed by microtubules. In this study, we treated human fibroblasts and human cancer cells (HeLa and ...

journal_title：Environmental and molecular mutagenesis

authors： Udroiu I,Coluzzi E,Bedini A,Giliberti C,Palomba R,Sgura A

更新日期：2019-08-01 00:00:00

abstract：:In this study we compared the ability of extractable organic material from particulate and semivolatile fractions of gasoline emission to induce mutations in bacteria and form adducts with calf thymus (CT) DNA with corresponding data obtained from diesel exhaust. Exhaust particles from gasoline-powered passenger cars ...

journal_title：Environmental and molecular mutagenesis

authors： Pohjola SK,Lappi M,Honkanen M,Savela K

更新日期：2003-01-01 00:00:00

abstract：:Heritable mutations may result in a wide variety of detrimental outcomes, from embryonic lethality to genetic disease in the offspring. Despite this, today's commonly used test batteries do not include assays for germ cell mutation. Current challenges include a lack of practical assays and concrete evidence for human ...

journal_title：Environmental and molecular mutagenesis

authors： Singer TM,Yauk CL

更新日期：2010-10-01 00:00:00

abstract：:DAS (diallyl sulfide), DADS (diallyl disulfide), and DATS (diallyl trisulfide) are major oil-soluble allyl sulfides (OAS) that represent major garlic constituents. The anticarcinogenic and antimutagenic effects of these substances have been extensively studied during the last decades. Previous reports suggest that ind...

journal_title：Environmental and molecular mutagenesis

authors： Wu XJ,Hu Y,Lamy E,Mersch-Sundermann V

更新日期：2009-04-01 00:00:00

abstract：:Riddelliine (RID) is a pyrrolizidine alkaloid found in plants of the genera Crotalaria, Amsinckia, and Senecio in the United States. RID has been extensively studied in a wide variety of in vitro short-term genotoxicity tests and has yielded positive responses in most test systems; however, there are fewer data availa...

journal_title：Environmental and molecular mutagenesis

authors： Mirsalis JC,Steinmetz KL,Blazak WF,Spalding JW

更新日期：1993-01-01 00:00:00

abstract：:DNA interstrand crosslinks (ICLs) are covalent linkages between two strands of DNA, and their presence interferes with essential metabolic processes such as transcription and replication. These lesions are extremely toxic, and their repair is essential for genome stability and cell survival. In this review, we will di...

journal_title：Environmental and molecular mutagenesis

authors： Sharma S,Canman CE

更新日期：2012-12-01 00:00:00

abstract：:Perturbation of DNA replication by chemical-DNA adducts produced by exposure to mutagenic/carcinogenic chemicals results in mutagenic or cytotoxic damage in the DNA. Demonstration of a correlation between cell cycle dependency of cytotoxicity and point mutation at the Na+/K+ ATPase gene could suggest that the two cons...

journal_title：Environmental and molecular mutagenesis

authors： Smith GJ,Grisham JW,Bentley KS

更新日期：1988-01-01 00:00:00

abstract：:In vitro transcriptional signatures that predict toxicities can facilitate chemical screening. We previously developed a transcriptomic biomarker (known as TGx-28.65) for classifying agents as genotoxic (DNA damaging) and non-genotoxic in human lymphoblastoid TK6 cells. Because TK6 cells do not express cytochrome P450...

journal_title：Environmental and molecular mutagenesis

authors： Yauk CL,Buick JK,Williams A,Swartz CD,Recio L,Li HH,Fornace AJ Jr,Thomson EM,Aubrecht J

更新日期：2016-05-01 00:00:00

abstract：:There is scientific and regulatory interest in using mutation assays in transgenic mice in safety assessments for new chemicals and drugs. Currently these assays are in the process of being validated, and protocols for routine testing are being defined. Some of the issues and results to date with regard to assay valid...

journal_title：Environmental and molecular mutagenesis

authors： Gorelick NJ

更新日期：1995-01-01 00:00:00

abstract：:Previously, we determined that elimination of deoxycytidylate (dCMP) deaminase (DCD1) in the yeast Saccharomyces cerevisiae increases the intracellular dCTP:dTTP ratio and reduces the induction of G x C --> A x T transitions in the SUP4-o gene by ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (M...

journal_title：Environmental and molecular mutagenesis

authors： Kunz BA,Henson ES,Karthikeyan R,Kuschak T,McQueen SA,Scott CA,Xiao W

更新日期：1998-01-01 00:00:00

abstract：:The cytotoxicity of streptozotocin (STZ) was investigated in Chinese hamster fibroblast lines (CHO-9 and V79) in comparison to two other alkylating agents, methylnitrosourea (MNU) and ethylnitrosourea (ENU), using cell survival as the endpoint. It was found that V79 cells were far more resistant to methylation induced...

journal_title：Environmental and molecular mutagenesis

authors： Capucci MS,Hoffmann ME,De Groot A,Natarajan AT

更新日期：1995-01-01 00:00:00

abstract：:The in vivo Pig-a mutation assay has been adapted for measuring mutation in rats, mice, monkeys, and humans. To date, the assay has been used mainly to assess the mutagenicity of chemicals that are known to be powerful point mutagens. The assay has not been used to measure the biological effects associated with ionizi...

journal_title：Environmental and molecular mutagenesis

authors： Ohtani S,Unno A,Ushiyama A,Kimoto T,Miura D,Kunugita N

更新日期：2012-10-01 00:00:00

abstract：:Chromosome aberrations can occur by secondary mechanism(s) associated with cytotoxicity, induced by chemicals that do not attack DNA. Aberrations are formed from DNA double-strand breaks, and DSBs are known to be induced by nonmutagenic (Ames test negative) noncarcinogens at toxic levels [Storer et al. (1996): Mutat R...

journal_title：Environmental and molecular mutagenesis

authors： Hilliard CA,Armstrong MJ,Bradt CI,Hill RB,Greenwood SK,Galloway SM

更新日期：1998-01-01 00:00:00

abstract：:Loss of heterozygosity (LOH) is a critical event in the development of human cancers. LOH is thought to result from either a large deletion or recombination between homologous alleles during repair of DNA double-strand breaks (DSBs). These types of genetic alterations produce mutations in the TK gene mutation assay, w...

journal_title：Environmental and molecular mutagenesis

authors： Honma M

更新日期：2005-03-01 00:00:00

abstract：:The mutational spectrum for 28 X-ray induced mutations and 2 spontaneous mutations, previously determined by genetic and cytogenetic methods, consisted of 20 multilocus deficiencies (19 induced and 1 spontaneous) and 10 intragenic mutations (9 induced and 1 spontaneous). One of the X-ray induced intragenic mutations w...

journal_title：Environmental and molecular mutagenesis

authors： Mahmoud J,Fossett NG,Arbour-Reily P,McDaniel M,Tucker A,Chang SH,Lee WR

更新日期：1991-01-01 00:00:00

abstract：:Treatment of AS52 cells with 5-azacytidine resulted in an induction of 6-thioguanine-resistant [6TG] colonies, which reached a maximum by an expression time of 9 days. Dose responses for both cytotoxicity and mutation induction were determined following treatment with 5-azacytidine. At 20 microM treatment, 5-azacytidi...

journal_title：Environmental and molecular mutagenesis

authors： Spencer DL,Caspary WJ,Hines KC,Tindall KR

更新日期：1996-01-01 00:00:00

abstract：:Micronucleus (MN) expression in peripheral blood lymphocytes is well established as a standard method for monitoring chromosome damage in human populations. The first results of an analysis of pooled data from laboratories using the cytokinesis-block micronucleus (CBMN) assay and participating in the HUMN (HUman Micro...

journal_title：Environmental and molecular mutagenesis

authors： Bonassi S,Fenech M,Lando C,Lin YP,Ceppi M,Chang WP,Holland N,Kirsch-Volders M,Zeiger E,Ban S,Barale R,Bigatti MP,Bolognesi C,Jia C,Di Giorgio M,Ferguson LR,Fucic A,Lima OG,Hrelia P,Krishnaja AP,Lee TK,Migliore L

更新日期：2001-01-01 00:00:00

abstract：:Metronidazole (MTZ) is used for the treatment of many infectious diseases, including vaginal infections. While data indicate that MTZ is mutagenic and induces micronuclei in rodents, there is no information on the genotoxicity of MTZ in epithelial vaginal cells or cervical cells. In the present study, we have instille...

journal_title：Environmental and molecular mutagenesis

authors： Ornelas-Aguirre JM,Gómez-Meda BC,Zamora-Perez AL,Ramos-Ibarra ML,Batista-González CM,Zúñiga-González GM

更新日期：2006-06-01 00:00:00

abstract：:Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. Conditions are presented for the routine sequencing of single- and dou...

journal_title：Environmental and molecular mutagenesis

authors： Zimmerman LJ,Fuscoe JC

更新日期：1991-01-01 00:00:00

abstract：:The genotoxic effects associated with automobile painting were analyzed using a panel of biomarkers. Chromosomal aberrations (CAs), sister chromatid exchange (SCE), and micronuclei were evaluated in 25 car painters (12 smokers, 13 nonsmokers) working in different automobile paint-shops in Italy and in 37 control subje...

journal_title：Environmental and molecular mutagenesis

authors： Testa A,Festa F,Ranaldi R,Giachelia M,Tirindelli D,De Marco A,Owczarek M,Guidotti M,Cozzi R

更新日期：2005-10-01 00:00:00

abstract：:It is known that N-nitrosopyrrolidine (NPYR), a carcinogen in rodents, is metabolically activated by microsomal cytochrome P450 to form an alpha-hydroxylated derivative, which induces mutations. The mutations have been demonstrated by use of Salmonella typhimurium and Escherichia coli. We discovered directly acting mu...

journal_title：Environmental and molecular mutagenesis

authors： Arimoto-Kobayashi S,Inada N,Anma N,Shimada H,Hayatsu H

更新日期：1999-01-01 00:00:00

abstract：:Maintenance of the mitochondrial genome is essential for proper cellular function. For this purpose, mitochondrial DNA (mtDNA) needs to be faithfully replicated, transcribed, translated, and repaired in the face of constant onslaught from endogenous and environmental agents. Although only 13 polypeptides are encoded w...

journal_title：Environmental and molecular mutagenesis

authors： Sharma N,Pasala MS,Prakash A

更新日期：2019-10-01 00:00:00

abstract：:The mitochondrial genome is essential for producing ATP (adenosine 5'-triphosphate) via oxidative phosphorylation. The gradual decline of mitochondrial function with age has long been postulated as a factor in aging. More recently, a variety of diseases have been related to molecular defects in human mitochondrial DNA...

journal_title：Environmental and molecular mutagenesis

authors： Grossman LI

更新日期：1995-01-01 00:00:00