Abstract:
BACKGROUND AND OBJECTIVE:LED photomodulation has been shown to profoundly influence cellular behavior. A variety of parameters with LED photomodulation can alter cellular response in vitro. The effects of one visible and one infrared wavelength were evaluated to determine the optimal ratio to produce a net increase in dermal collagen by altering the ratio of total energy output of each wavelength. The ratio between the two wavelengths (590 and 870 nm) was shifted in 25% increments. STUDY DESIGN/MATERIALS AND METHODS:Human skin fibroblasts in culture were exposed to a 590/870 nm LED array with total combined energy density fixed at 4.0 mW/cm.. The ratio of 590/870 nm tested parameters were: 100/0%, 75/25%, 50/50%, 25/75%, and 0/100%. These ratios were delivered using pulsed duty cycle of exposure (250 milliseconds "on" time/100 milliseconds "off" time/100 pulses) for a total energy fluence of 0.1 J/cm.. Gene expression was examined using commercially available extra cellular matrix and adhesion molecule RT PCR Arrays (SA Biosciences, Frederick, MD) at 24 hours post-exposure. RESULTS:Different expression profiles were noticed for each of the ratios studied. Overall, there was an average (in an 80 gene array) of 6% expression difference in up or downregulation between the arrays. The greatest increase in collagen I and decrease in collagenase (MMP-1) was observed with 75/25% ratio of 590/870 nm. The addition of increasing proportions of IR wavelengths causes alteration in gene expression profile. The ratios of the wavelengths caused variation in magnitude of expression. CONCLUSIONS:Cell metabolism and gene expression can be altered by simultaneous exposure to multiple wavelengths of low energy light. Varying the ratios of specific wavelength intensity in both visible and near infrared light therapy can strongly influence resulting fibroblast gene expression patterns.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
McDaniel DH,Weiss RA,Geronemus RG,Mazur C,Wilson S,Weiss MAdoi
10.1002/lsm.20947subject
Has Abstractpub_date
2010-08-01 00:00:00pages
540-5issue
6eissn
0196-8092issn
1096-9101journal_volume
42pub_type
杂志文章abstract::Photochemical internalization (PCI) is a method for intracellular delivery of hydrophilic macromolecular drugs with intracellular targets as well as other drugs with limited ability to penetrate cellular membranes. Such drugs enter cells by means of endocytosis and are to a large extent degraded by hydrolytic enzymes ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,评审
doi:10.1002/lsm.21084
更新日期:2011-09-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Swelling and trismus are complications experienced by nearly all subjects after undergoing oral surgery for the removal of impacted teeth. The main purpose of this paper was to compare the effects of photobiomodulation therapy (PBMT) at two different wavelengths applied intra-orally and extra-o...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22488
更新日期:2016-07-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:High-power laser has recently become a physical stimulus for bone regeneration. Little is known about how high-power laser irradiation affects osteoblast differentiation. This study investigated osteoblast responses to high-power laser and combined irradiation with BMP-2 treatment. STUDY DESIG...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20870
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Acne scarring is a common disfiguring sequela of acne vulgaris which can lead to serious psychosocial problems and have a negative effect on patients' quality of life. Although a variety of approaches can be used to treat atrophic acne scars, disadvantages such as long-healing time, dyspigmentation, infectio...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22825
更新日期:2018-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Interstitial photodynamic therapy (iPDT) of non-resectable recurrent glioblastoma using 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) has shown a promising outcome. It remained unclear, however, to what extent inter- and intra-tumoural differences of PpIX concentrations influence...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.22126
更新日期:2013-04-01 00:00:00
abstract::Seven cases of cerebral tumors (four deep-seated sovratentorial gliomas located in motor area, occipital region, parietal region and frontal region; one deep-seated cystic cerebellar spongioblastoma; one sphenoidal wing meningioma, and one spinal cord intradural tumor) were simultaneously irradiated in the same area w...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900020205
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Understanding albumin solder denaturation is important for laser tissue soldering. Human (HSA), bovine (BSA), porcine (PSA), and canine (CSA) albumin both fatty acid containing (FAC) and fatty acid free (FAF) were evaluated by using differential scanning calorimetry (DSC). STUDY DESIGN/MATERIA...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)27:5<465::AID-LSM1007>3.0.C
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Port-wine stain (PWS) is a congenital, progressive vascular malformation of the dermis. The use of optical coherence tomography (OCT) for the characterization of blood vessels in PWS skin has been demonstrated by several groups. In the past few years, advances in OCT technology have greatly in...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22194
更新日期:2013-12-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The recently introduced Er:YAG laser seems to be a promising alternative in periodontal treatment due to its thermo-mechanical ablation mechanism. The present study attempted to compare the effects of an Er:YAG laser on periodontally involved root surfaces at different power settings in vivo an...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章
doi:10.1002/lsm.1125
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Pulsed dye laser (PDL) is the first choice for treatment of port wine stains (PWS). However, outcome is highly variable and only a few patients achieve complete clearance. The objective of the study was to compare efficacy and safety of single pass PDL with double pass PDL at a 6 minute interval. METHODS:We...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,随机对照试验
doi:10.1002/lsm.22011
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Previously, we have shown that a 43°C pretreatment can provide thermotolerance to a following, more severe, thermal stress at 45°C. Using cells that lack the Hsp70 gene, we have also shown that there is still some thermotolerance in the absence of HSP70 protein. The purpose of this study was t...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20983
更新日期:2010-12-01 00:00:00
abstract::Percutaneous endoscopic approaches to cholelithiasis would be facilitated by methods for welding gallbladder tissues. We evaluated the bursting pressure and histologic appearance of canine gallbladder tissue welded with a thulium-holmium-chromium:YAG (THC:YAG) laser producing a 2.15 microns pulsed output. The tissue a...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090211
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Carbon dioxide (CO(2)) laser resurfacing is primarily performed on photodamaged facial skin where patients are further exposed to ultraviolet radiation (UVR) postoperatively. We examined whether pre- and postoperative UVR influences the development of CO(2) laser-induced side effects. STUDY D...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20135
更新日期:2005-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:We developed novel heat-conducting templates, and tested whether they could effectively remove damaging heat from the tissue during laser ablation. The reduction of lateral thermal damage during cutaneous incisional laser procedures should decrease the time in wound healing. In addition, we se...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.10022
更新日期:2002-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:A semi-solid albumin solder formulated with hydroxypropylmethylcellulose (HPMC) was designed to improve the characteristics of liquid and solid solders. STUDY DESIGN/MATERIALS AND METHODS:Acute tensile strengths were determined on canine small bowel in vitro by using liquid 50% bovine serum al...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/1096-9101(2000)27:2<140::aid-lsm5>3.0.co;2
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Laser resurfacing with the 950 micros pulsed CO(2) laser is an effective treatment for photodamage and acne scarring; however, the potential for prolonged erythema and delayed re-epithelialization dissuade many patients from the procedure. With the use of erbium lasers alone, there is a decreas...
journal_title:Lasers in surgery and medicine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/lsm.1099
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Linear birefringence is an anisotropic property of rat tail tendon, which is largely composed of collagen. Our goal is to show that the dynamic range and sensitivity of the linear birefringence loss of collagen during heating are sufficient for kinetic modeling of the reaction. STUDY DESIGN, M...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1997)20:3<310::aid-lsm10>3
更新日期:1997-01-01 00:00:00
abstract::CO2, Nd:YAG, and argon lasers have been used for 5 years for experimental and clinical procedures at the National Cancer Institute of Milan under the auspices of the National Research Council. Experimental data regarding tumor cell spread through lymphatic and hematic channels and cell viability in the fumes after CO2...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900020409
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Urinary incontinence is a common and distressing condition which interferes with everyday life. Patients frequently experience discomfort related to urine leakage and the subsequent need to use absorbent pads. Since the continence mechanism is primarily maintained by a proper function of pelvi...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章,多中心研究
doi:10.1002/lsm.23106
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Lasers are used for caries removal in enamel and dentin, but are currently limited in their ability to remove sound tooth structure. In this study plasma interaction regimes are investigated as the principal factor determining the ablation characteristics. STUDY DESIGN/MATERIALS AND METHODS:Th...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)18:2<121::AID-LSM1>3.
更新日期:1996-01-01 00:00:00
abstract::Hyperthermia induced by a microwave source (2,450 MHz) was used alone and in combination with photodynamic therapy (PDT) to treat the SMT-F, EMT-6, and RIF animal tumors in vivo. PDT was administered using either Photofrin I or II as the photosensitizer and an argon-pumped tunable dye laser (630 nm) as the light sourc...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900070104
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:The effects of Er:YAG laser irradiation on dentin have not been sufficiently investigated. The purpose of this study was to investigate the effects of Er:YAG laser irradiation on dentin. STUDY DESIGN/MATERIALS AND METHODS:After cavities were prepared using Er:YAG laser irradiation or rotary c...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.10061
更新日期:2002-01-01 00:00:00
abstract::Ablative lasers (CO2 and Er:YAG) provide the greatest improvement in photoaging, but significant adverse effects limit their use. Nonablative lasers have reduced adverse effects, but limited efficacy. Fractional photothermolysis (FP) produces arrays of microscopic thermal wounds called microscopic treatment zones (MTZ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.20310
更新日期:2006-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:To demonstrate that high color fidelity light-emitting diode (LED) sources are preferred by dermatologists for the evaluation of patients during standard-of-care, outpatient visits when compared to low color fidelity LED sources similar to fluorescent lighting. STUDY DESIGN/MATERIALS AND METH...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23352
更新日期:2020-11-08 00:00:00
abstract::We report the first study of the application of excimer lasers in dentistry for the treatment of dental root canals. High-energy ultraviolet (UV) radiation emitted by an XeCl excimer laser (308 nm) and delivered through suitable optical fibers can be used to remove residual organic tissue from the canals. To this aim,...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090407
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Fifty percent human albumin solder significantly improves weld strength when compared to lower concentrations [Wright et al., ASLMS meeting, April, 1995]. We developed a method for preparing 50% human albumin that may be considered compatible for clinical applications. STUDY DESIGN/MATERIALS A...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(SICI)1096-9101(1996)19:1<2::AID-LSM1>3.0.
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effect of relatively novel approach of application of polychromatic light waves on flap survival of experimental musculocutaneous flap model and to investigate efficacy of this modality as a delay procedure to increase vascularization of zone 4 of transverse rectus ab...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.23063
更新日期:2019-08-01 00:00:00
abstract::The pulsed dye laser (wavelength 504 nm, pulse duration 1 microsecond) is widely used for fragmenting urinary and biliary calculi. In this study, the performance of this laser was compared with pulsed dye lasers producing pulse durations of 8 and 20 microseconds. Fragmentation thresholds and fragmentation rates were m...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.1900090505
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Knowledge about optical parameters and the resultant light distribution in laser-treated tissue is important for predicting the effects of laser-induced thermotherapy of liver metastases (LITT). MATERIALS AND METHODS:The absorption and scattering coefficients as well as the anisotropy factors ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/(sici)1096-9101(1998)23:4<194::aid-lsm2>3.
更新日期:1998-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Many studies have investigated the application of micro-insulated needles with radio frequency (RF) to treat acne in humans; however, the use of a micro-insulated needle RF applicator has not yet been studied in an animal model. The purpose of this study was to evaluate the effectiveness of a ...
journal_title:Lasers in surgery and medicine
pub_type: 杂志文章
doi:10.1002/lsm.22599
更新日期:2017-04-01 00:00:00