Abstract:
OBJECTIVES:To develop, evaluate and implement a new multiplex real-time PCR test for the detection of herpes simplex virus (HSV)1, HSV2 and syphilis in a single sample using a single test. METHODS:A multiplex real-time PCR test detecting HSV1, HSV2 and Treponema pallidum was designed, validated and evaluated for a period of 6 months on patients attending the Sandyford Initiative (a series of genitourinary medicine clinics in and around Glasgow). A total of 692 samples were tested, and T pallidum PCR positives were confirmed by a second PCR at the Scottish Reference Laboratory (SBSTIRL). All PCR results were aligned with dark ground microscopy findings and serological results where available and compared. RESULTS:The laboratory validation of the multiplex assay showed the test to be sensitive, specific and robust. Of the 692 samples, 139 were positive for HSV1, 136 for HSV2, 15 for syphilis, one for both syphilis and HSV1, and 401 were negative; the reference laboratory confirmed all T pallidum PCR-positive samples. The PCR test was more sensitive than both dark ground microscopy and serological testing for the diagnosis of primary syphilis. CONCLUSIONS:The introduction of this new test has led to a better turnaround time for the diagnosis of genital ulcer disease, better detection of primary syphilis infection, and the detection of unexpected cases of syphilis where the aetiological agent suspected was HSV.
journal_name
Sex Transm Infectjournal_title
Sexually transmitted infectionsauthors
Scott LJ,Gunson RN,Carman WF,Winter AJdoi
10.1136/sti.2009.040451subject
Has Abstractpub_date
2010-12-01 00:00:00pages
537-9issue
7eissn
1368-4973issn
1472-3263pii
sti.2009.040451journal_volume
86pub_type
杂志文章abstract:OBJECTIVES:To assess attitudes to HIV risk and acceptability of rapid HIV testing among clients of street-based female sex workers (FSW) in Lausanne, Switzerland, where HIV prevalence in the general population is 0.4%. METHODS:The authors conducted a cross-sectional study in the red light district of Lausanne for five...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2012-050489
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:STIs during pregnancy increase adverse pregnancy and birth outcomes and may increase HIV risk. STI syndromic management is standard of care in South Africa. Our study evaluated the prevalence and incidence of STIs in pregnant women and the associated risk factors. METHODS:We combined data from two prospectiv...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2020-054631
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVES:In recent years, studies have demonstrated frequent rectal Chlamydia trachomatis (CT) detection in women, irrespective of reported anal sex or rectal symptoms. However, the clinical relevance and public health implication of rectal CT detection in women remain under debate. Therefore, evaluating CT viability...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2019-054002
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:To determine trends and correlates of chlamydia positivity among young heterosexuals attending Australian sexual health services and to compare these with population-based notification data. METHODS:Data from 18 sexual health services and the national notification scheme were analysed. A χ2 test assessed tr...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050423
更新日期:2012-11-01 00:00:00
abstract:OBJECTIVES:To explore the bodily and relational experience of taking antiretroviral therapy (ART) and the subsequent effect on retention in HIV care in six sub-Saharan African countries. METHODS:In-depth interviews were conducted with 130 people living with HIV (PLHIV) who had initiated ART, 38 PLHIV who were lost to ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sextrans-2016-052971
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVE:To investigate the pathogen profile in men with inflammation of the paraurethral glands. METHODS:We enrolled 40 patients with first-onset, drug-naive inflammation of the paraurethral glands. Discharge from the lesions was collected for Gram staining. Microscopy was performed for screening for trichomonads an...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2013-051180
更新日期:2014-02-01 00:00:00
abstract:BACKGROUND:Almost half of all new HIV infections in Thailand occur among low-risk partners of people infected with HIV, so it is important to include people infected with HIV in prevention efforts. METHODS:Risk for HIV transmission was assessed among people with HIV attending routine care at the National Infectious Di...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2008.032532
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVE:Three decades into the HIV epidemic and with the advancement of HIV treatments, condom and non-condom-based anal intercourse among gay men in resource-rich countries needs to be re-assessed. METHODS:The proportions of men engaging in a range of anal intercourse practices were estimated from the ongoing cross...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050041
更新日期:2011-10-01 00:00:00
abstract:BACKGROUND:Identifying predictors that contribute to geographical disparities in sexually transmitted infections (STIs) is necessary. This study assesses the spatial relationship between condom availability to locations of STIs in order to better understand these geographical disparities. OBJECTIVES:We conducted a con...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052144
更新日期:2016-05-01 00:00:00
abstract:OBJECTIVES:The objective of this retrospective study was to summarise the clinical manifestations of, and to analyse the incidence and risk factors of, Jarisch-Herxheimer reaction (JHR) during the treatment of children with symptomatic congenital syphilis. METHODS:Clinical data of 60 children with clinically and labor...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2016-053083
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:To assess the validity of sexual behaviour data collected from African adolescents using five methods. METHODS:9280 Tanzanian adolescents participated in a biological marker and face to face questionnaire survey and 6079 in an assisted self-completion questionnaire survey; 74 participated in in-depth intervi...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2004.011924
更新日期:2004-12-01 00:00:00
abstract:OBJECTIVES:To measure the prevalence of HSV-2 antibodies in a birth cohort of 21 year old New Zealanders from whom detailed sexual histories had been obtained, and to assess the potential for HSV-2 serology in characterising a young adult population's risk for sexually transmitted diseases (STD). METHODS:Sera from 784...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.74.3.216
更新日期:1998-06-01 00:00:00
abstract:OBJECTIVE:To determine whether Mycoplasma genitalium is associated with balanitis and/or posthitis in a previous study of the role of M genitalium in men with acute non-gonococcal urethritis (NGU). METHODS:In a previous study of men with acute NGU, the existence of balanitis and/or posthitis was recorded. Chlamydia tr...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2010.044487
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND:Vulvo-vaginal swabs (VVSs) are not validated for use by the manufacturers of two widely used nucleic acid amplification tests (NAAT) for the detection of Chlamydia trachomatis. However, there is evidence that this type of swab is suitable for diagnosis. OBJECTIVE:To validate the Cobas Taqman CT assay for th...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2007.029587
更新日期:2008-08-01 00:00:00
abstract:OBJECTIVES:To assess associations between residences location, risky sexual behaviours and sexually transmitted diseases (STDs) among adults living in Guangzhou, China. METHODS:Data were obtained from 751 Chinese adults aged 18-59 years in Guangzhou, China, using stratified random sampling by using spatial epidemiolog...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052268
更新日期:2016-06-01 00:00:00
abstract:OBJECTIVE:To determine the seroprevalence rate of syphilis among pregnant women attending the antenatal clinics of a teaching and a state specialist hospital in Nigeria, in order to ascertain whether maternal screening should be incorporated into routine antenatal care of our hospitals. METHODS:A screening for syphili...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2006.023416
更新日期:2007-08-01 00:00:00
abstract:OBJECTIVE:To pilot and evaluate sexually transmitted infection (STI) management in community family planning clinics (FPCs). METHODS:Number of STI tests taken, positive results, infections treated, contacts traced/treated, referrals to specialist services and time from testing to treatment were documented as well as a...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2003.004382
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND:Adults in developing countries frequently use community pharmacies as the first and often only source of care. The objective of this study was to assess the success of pharmacy referrals and uptake of HIV testing by young adult clients of community pharmacies in the context of a screening programme for acute...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2014-051751
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:To develop an analytical understanding of non-cohabiting sex partnering in sub-Saharan Africa (SSA) using nationally representative sexual behaviour data. METHOD:A non-homogenous Poisson stochastic process model was used to describe the dynamics of non-cohabiting sex. The model was applied to 25 countries in...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2014-051925
更新日期:2015-09-01 00:00:00
abstract::We present eight cases and review the literature of concurrent urethritis and conjunctivitis where adenovirus was identified as the causative pathogen. The focus of this review concerns the identification of specific sexual practices, symptoms, signs and any serotypes that seem more commonly associated with such adeno...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sextrans-2014-051868
更新日期:2015-03-01 00:00:00
abstract:OBJECTIVE:To study the validity and performance of a number of rapid indicators for the diagnosis of sexually transmitted infections (STIs) in female prostitutes in Dakar, Senegal; characteristics of these indicators were rapidly obtainable, easy to perform, accurate, useful at district level, and reasonable cost. MET...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract:BACKGROUND:Screening has been recommended to reduce the prevalence and morbidity associated with genital chlamydia infection in the United Kingdom. METHODS:We describe the rationale and study design of the Chlamydia Screening Studies (ClaSS), a collaborative project designed to evaluate screening outside genitourinary...
journal_title:Sexually transmitted infections
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1136/sti.2003.006197
更新日期:2004-10-01 00:00:00
abstract:OBJECTIVES:In 2006, a new variant of Chlamydia trachomatis (nvCT) was reported in Sweden. Because of a cryptic plasmid deletion, the nvCT was undetectable in several of the genetic diagnostic systems used worldwide at the time. This study aimed to evaluate whether the nvCT was present in specimens obtained from patient...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2013-051018
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:From December 2017, the Australian National Cervical Screening Program commenced 5 yearly primary human papillomavirus (HPV) screening; one of the first high-income countries to implement primary HPV screening. This study aimed to examine the psychosocial impact of self-reporting testing HPV positive in a sam...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2019-054290
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:AIDS is the main driver of young widowhood in southern Africa. METHODS:The demographic characteristics of widows, their reported risk behaviours and the prevalence of HIV were examined by analysing a longitudinal population-based cohort of men and women aged 15-54 years in Manicaland, eastern Zimbabwe. The ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2008.033043
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVES:This study aimed to describe the epidemiology of HIV infection and sexually transmitted infections (STIs) among persons who attended voluntary counselling and testing (VCT) service for HIV and to assess whether the VCT programme reached the right population at risk in Taiwan. METHODS:From 2006 to 2010, ques...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050354
更新日期:2012-12-01 00:00:00
abstract:BACKGROUND:Intravaginal practices including vaginal washing have been associated with HIV-1 acquisition. This association may be mediated by mucosal disruption, changes in vaginal flora or genital tract inflammatory responses. Reducing vaginal washing could lower women's risk of HIV-1 acquisition. METHODS:23 HIV-1 ser...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2012-050564
更新日期:2013-05-01 00:00:00
abstract:OBJECTIVES:Men who have sex with men (MSM) are at increased risk for STIs and mental disorders. Syndemic theory holds that psychosocial issues co-occur and interact, and thus increase sexual risk behaviour. Psychosocial issue identification, referral and management might reduce risk behaviour. METHODS:In the syndemic-...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2020-054438
更新日期:2021-02-01 00:00:00
abstract:OBJECTIVES:To validate a simplified grading scheme for Gram stained smears of vaginal fluid for the diagnosis of bacterial vaginosis (BV) against the accepted "gold" standard of Amsel's composite criteria. METHODS:Women attending genitourinary medicine (GUM) clinics, as part of a multicentre study, were diagnosed as h...
journal_title:Sexually transmitted infections
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1136/sti.78.6.413
更新日期:2002-12-01 00:00:00
abstract:OBJECTIVES:To determine the prevalence of HIV and associated risk factors among female sex workers (FSWs) in border provinces of Vietnam. METHODS:911 FSWs in five border provinces of Vietnam (Lai Chau, Quang Tri, Dong Thap, An Giang, and Kien Giang) were enrolled in a cross sectional study. Subjects were interviewed u...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2005.016097
更新日期:2005-12-01 00:00:00