Validation of a simplified grading of Gram stained vaginal smears for use in genitourinary medicine clinics.

Abstract:

OBJECTIVES:To validate a simplified grading scheme for Gram stained smears of vaginal fluid for the diagnosis of bacterial vaginosis (BV) against the accepted "gold" standard of Amsel's composite criteria. METHODS:Women attending genitourinary medicine (GUM) clinics, as part of a multicentre study, were diagnosed as having BV if three or more of the following criteria were present; homogeneous discharge, elevated vaginal pH, production of amines, and presence of "clue" cells. Women with less than three of the criteria were considered as normal. Simultaneously, smears were made of vaginal fluid and Gram stained and then assessed qualitatively as normal (grade I), intermediate (grade II), or consistent with BV (grade III). Two new grades were used, grade 0, epithelial cells only with no bacteria, and grade IV, Gram positive cocci only. RESULTS:BV was diagnosed in 83/162 patient visits using the composite criteria, the remainder being regarded as normal. The majority of patients with BV had a smear assessed as grade III (80/83, 96%) and the majority of normal women had a smear assessed as grade I (normal, 48/79, 61%), giving a high sensitivity (97.5%), specificity (96%), and predictive value for a positive (94.1%) and negative (96%) test, kappa index = 0.91. Smears assessed as grade II were found predominantly (12/13) among patients diagnosed as normal, with less than three of the composite criteria. Grades 0 and IV were both only found among normal women. CONCLUSION:This simplified assessment of Gram stained smears can be used as an alternative to Amsel's criteria and is more applicable for use in busy GUM clinics.

journal_name

Sex Transm Infect

authors

Ison CA,Hay PE

doi

10.1136/sti.78.6.413

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

413-5

issue

6

eissn

1368-4973

issn

1472-3263

journal_volume

78

pub_type

临床试验,杂志文章,多中心研究,随机对照试验
  • Global epidemiology of Trichomonas vaginalis.

    abstract::Despite having the highest prevalence of any sexually transmitted infection (STI) globally, there is a dearth of data describing Trichomonas vaginalis (TV) incidence and prevalence in the general population. The lack of basic epidemiological data is an obstacle to addressing the epidemic. Once considered a nuisance in...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,评审

    doi:10.1136/sextrans-2013-051075

    authors: Poole DN,McClelland RS

    更新日期:2013-09-01 00:00:00

  • Escalation in the relative prevalence of ciprofloxacin-resistant gonorrhoea among men with urethral discharge in two South African cities: association with HIV seropositivity.

    abstract:OBJECTIVES:The objectives of this study were to assess the prevalence of ciprofloxacin-resistant gonorrhoea in two South African cities and to investigate the association between the isolation of ciprofloxacin-resistant Neisseria gonorrhoeae and the HIV serostatus of patients. METHODS:Gonococci were cultured from endo...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,多中心研究

    doi:10.1136/sti.2007.029611

    authors: Lewis DA,Scott L,Slabbert M,Mhlongo S,van Zijl A,Sello M,du Plessis N,Radebe F,Wasserman E

    更新日期:2008-10-01 00:00:00

  • Meeting sexual partners online: associated sexual behaviour and prevalent chlamydia infection among adolescents in Norway: a cross-sectional study.

    abstract:OBJECTIVES:Evidence is mixed as to whether meeting sexual partners online ('internet-partners') is associated with risky sexual behaviour and/or sexually transmitted infection transmission. Accordingly, we sought to estimate the prevalence of reporting various online romantic and sexual activities among Norwegian adole...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2015-052152

    authors: Gravningen K,Aicken CR,Schirmer H,Mercer CH

    更新日期:2016-03-01 00:00:00

  • The superiority of polymerase chain reaction over an amplified enzyme immunoassay for the detection of genital chlamydial infections.

    abstract:BACKGROUND/OBJECTIVES:The polymer conjugate enhanced enzyme immunoassay (IDEIA) and Cobas Amplicor polymerase chain reaction Chlamydia trachomatis (CT) (Amplicor PCR) are two commonly used assays for the diagnosis of CT infection. The performance of these assays was compared for the diagnosis of genital CT infection am...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.2005.015362

    authors: Jalal H,Stephen H,Al-Suwaine A,Sonnex C,Carne C

    更新日期:2006-02-01 00:00:00

  • Prospective study on cervical neoplasia: presence of HPV DNA in cytological smears precedes the development of cervical neoplastic lesions.

    abstract:OBJECTIVES:The principal aim of the study was to verify whether HPV infection in healthy women, as determined by HPV DNA detection, was associated with an increased risk of development of cervical lesions. METHODS:Cervical smears collected at enrolment into the prospective study conducted in Prague during 1975-83 were...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.79.3.191

    authors: Tachezy R,Saláková M,Hamsíková E,Kanka J,Havránková A,Vonka V

    更新日期:2003-06-01 00:00:00

  • Prevalence of sexual dysfunction in new heterosexual attenders at a central London genitourinary medicine clinic in 1998.

    abstract:OBJECTIVE:To determine the prevalence of sexual dysfunction and dissatisfaction (SD) among new heterosexual attendees at a central London genitourinary medicine (GUM) clinic. METHODS:Consecutive new attendees seen by a single clinician were asked a probe question about SD in the previous year. A clinical interview fol...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.76.3.208

    authors: Goldmeier D,Judd A,Schroeder K

    更新日期:2000-06-01 00:00:00

  • Superimposed primary chancre in a patient with Adamantiades-Behçet's disease.

    abstract::Adamantiades-Behçet's disease was diagnosed in a 42 year old Turkish patient with recurrent oral aphthae, genital ulcerations, papules, and sterile pustules, histologically presenting as cutaneous vasculitis, and intermittent arthritis with joint effusion particularly of the knees. Six months after initial improvement...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.75.2.124

    authors: Meljanac N,Dippel E,Zouboulis CC

    更新日期:1999-04-01 00:00:00

  • Risky sexual behaviour in context: qualitative results from an investigation into risk factors for seroconversion among gay men who test for HIV.

    abstract:OBJECTIVES:The INSIGHT case-control study confirmed that HIV serodiscordant unprotected anal intercourse (SdUAI) remains the primary risk factor for HIV infection in gay men in England. This paper uses qualitative follow-up data to examine the contexts of SdUAI and other risk factors among the case-control study partic...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,多中心研究

    doi:10.1136/sti.2008.031468

    authors: Elam G,Macdonald N,Hickson FC,Imrie J,Power R,McGarrigle CA,Fenton KA,Gilbart VL,Ward H,Evans BG,INSIGHT Collaborative Research Team.

    更新日期:2008-11-01 00:00:00

  • Oral examination: a screening tool for HIV infection?

    abstract:OBJECTIVE:To estimate the predictive values for HIV infection of diagnosis of oral manifestations of the infection. METHOD:Prevalence of oral manifestations was compared in cross sectional blinded clinical examinations of homosexual men attending a genitourinary medicine clinic. Data were extrapolated to populations i...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.74.5.345

    authors: Robinson PG,Challacombe SJ,Sheiham A

    更新日期:1998-10-01 00:00:00

  • Mycoplasma genitalium is not associated with adverse outcomes of pregnancy in Guinea-Bissau.

    abstract:OBJECTIVE:To evaluate the impact of Mycoplasma genitalium on the outcome of pregnancy. METHODS:Cervical samples from women who had previously participated in a case-control study (designed to assess the impact of syphilis and HIV-2 on the outcome of pregnancy in Guinea-Bissau) were processed using a PCR assay to detec...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.78.4.289

    authors: Labbé AC,Frost E,Deslandes S,Mendonça AP,Alves AC,Pépin J

    更新日期:2002-08-01 00:00:00

  • Discordance of HIV and HSV-2 biomarkers and self-reported sexual behaviour among orphan adolescents in Western Kenya.

    abstract:BACKGROUND:This paper examines the discordance between biological data of HIV and herpes simplex virus type 2 (HSV-2) infections and self-reported questionnaire responses among orphan adolescents in Western Kenya. METHODS:In 2011, 837 orphan adolescents from 26 primary schools were enrolled in an HIV prevention trial....

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,多中心研究

    doi:10.1136/sextrans-2014-051720

    authors: Cho H,Luseno W,Halpern C,Zhang L,Mbai I,Milimo B,Hallfors DD

    更新日期:2015-06-01 00:00:00

  • Mycoplasma genitalium is associated with symptomatic and asymptomatic non-gonococcal urethritis in men.

    abstract:OBJECTIVES:To examine the prevalence of Mycoplasma genitalium in a large number of male patients attending a sexually transmitted infections (STI) clinic and to determine if there is an association with objective non-gonococcal urethritis (NGU) in patients with and without clinical symptoms. METHODS:Patients were test...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.2008.032730

    authors: Moi H,Reinton N,Moghaddam A

    更新日期:2009-02-01 00:00:00

  • Hormonal contraception and risk of STIs and bacterial vaginosis in South African adolescents: secondary analysis of a randomised trial.

    abstract:OBJECTIVES:Young women in sub-Saharan Africa are at high risk of STIs and unintended pregnancies, yet hormonal contraceptive (HC) use may affect STI risk. We compared the influence of three HCs on the incidence and prevalence of STIs and bacterial vaginosis (BV) in South African adolescents. METHODS:One hundred and th...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2020-054483

    authors: Balle C,Gill K,Konstantinus IN,Jaumdally SZ,Lennard K,Esra R,Happel AU,Barnabas SL,Gamieldien H,Pidwell T,Maseko V,Lesosky M,Myer L,Passmore JS,Bekker LG,Jaspan HB

    更新日期:2020-09-28 00:00:00

  • A novel condom policy for young attenders at a sexual health clinic.

    abstract:OBJECTIVES:To review the uptake of a new service for condom provision in the under 16s. METHODS:A retrospective case note review of attenders at an inner London sexual health clinic. RESULTS:The service was audited over a 3 month period in the spring of 2000. There were 97 individual attendances, 94% (92) male and 6%...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.77.4.287

    authors: Wright S,Tobin R,Kell P,Franks J

    更新日期:2001-08-01 00:00:00

  • Patterns of chlamydia testing in different settings and implications for wider STI diagnosis and care: a probability sample survey of the British population.

    abstract:BACKGROUND:Following widespread rollout of chlamydia testing to non-specialist and community settings in the UK, many individuals receive a chlamydia test without being offered comprehensive STI and HIV testing. We assess sexual behaviour among testers in different settings with a view to understanding their need for o...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2016-052719

    authors: Clifton S,Mercer CH,Woodhall SC,Sonnenberg P,Field N,Lu L,Johnson AM,Cassell JA

    更新日期:2017-06-01 00:00:00

  • Demographic changes and trends in risk behaviours, HIV and other sexually transmitted infections among female sex workers in Bangalore, India involved in a focused HIV preventive intervention.

    abstract::The primary objectives of this study were to assess the changing demographic characteristics of female sex workers (FSWs) in the urban Bangalore district, India, and trends in programme coverage, HIV/sexually transmitted infection prevalence rates and condom use. Cross-sectional, integrated behavioural and biological ...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2013-051203

    authors: Jayaraman GC,Kumar S,Isac S,Javalkar P,Gowda PR,Raghunathan N,Gowda CS,Bhattacharjee P,Moses S,Blanchard JF

    更新日期:2013-12-01 00:00:00

  • Diagnostic implications of 16S ribosomal assay for gonorrhoea.

    abstract:OBJECTIVES:In the absence of a single nucleic acid amplification test (NAAT) that is both highly specific and sensitive for gonorrhoea, many have put forward the 16S-based assay as a confirmatory test for Neisseria gonorrhoeae. This study was undertaken to evaluate the performance of PCR based on 16S ribosomal gene in ...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.2009.041418

    authors: Verma R,Sood S,Bala M,Kapil A,Das BK,Sharma VK,Samantaray JC

    更新日期:2010-11-01 00:00:00

  • Characterisation of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing in a Swedish county before and after identification of the new variant.

    abstract:OBJECTIVES:In 2006 a new variant of Chlamydia trachomatis (nvCT), with a deletion in the cryptic plasmid, was reported in Sweden. This deletion included the targets for the genetic diagnostic systems used in many clinical laboratories and resulted in thousands of false-negative results. The aim of this study was to cha...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.2009.037572

    authors: Jurstrand M,Christerson L,Klint M,Fredlund H,Unemo M,Herrmann B

    更新日期:2010-02-01 00:00:00

  • Oral contraceptive use may not preclude condom use: a study of non-pregnant African-American adolescent females.

    abstract:OBJECTIVE:To determine the association between oral contraceptive and condom use, and laboratory-confirmed sexually transmitted infection (STI) among African-American adolescent females at a high risk of STI acquisition. METHODS:A cross-sectional study of 715 African-American adolescent females (15-21 years old) was c...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.2006.022442

    authors: Crosby RA,DiClemente RJ,Wingood GM,Salazar LF,Rose E,Sales JM,Caliendo AM

    更新日期:2007-06-01 00:00:00

  • Prevalence of HIV-1 among attenders at sexually transmitted disease clinics: analyses according to country of birth.

    abstract:OBJECTIVES:To determine the importance of world region of birth as a risk factor for HIV-1 infection, the likelihood of having an HIV-1 infection diagnosed and the likelihood of having another coexisting acute sexually transmitted infection (STI) among attenders at genitourinary medicine clinics. SUBJECTS:Specimens fr...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.74.6.415

    authors: McGarrigle CA,Nicoll A

    更新日期:1998-12-01 00:00:00

  • HIV testing and self-reported HIV status in South African men who have sex with men: results from a community-based survey.

    abstract:OBJECTIVE:To investigate the characteristics of South African men who have sex with men (MSM) who (1) have been tested for HIV and (2) are HIV positive. METHODS:Data were collected from 1045 MSM in community surveys using questionnaires that were administered either face-to-face, by mail or on the internet. The mean a...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,多中心研究

    doi:10.1136/sti.2008.031500

    authors: Sandfort TG,Nel J,Rich E,Reddy V,Yi H

    更新日期:2008-11-01 00:00:00

  • Age at first anal sex and HIV/STI vulnerability among gay men in Australia.

    abstract:OBJECTIVES:To determine whether there is a link between age at first anal intercourse (AFAI) and gay men's HIV/sexually transmissible infection (STI) vulnerability, including tendencies to engage in higher risk sexual behaviour. METHODS:A nationwide cross-sectional survey was conducted online involving 845 Australian ...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2011-050253

    authors: Lyons A,Pitts M,Grierson J,Smith A,McNally S,Couch M

    更新日期:2012-06-01 00:00:00

  • Which azithromycin regimen should be used for treating Mycoplasma genitalium? A meta-analysis.

    abstract:BACKGROUND:There is increasing evidence that azithromycin 1 g is driving the emergence of macrolide resistance in Mycoplasma genitalium worldwide. We undertook a meta-analysis of M. genitalium treatment studies using azithromycin 1 g single dose and azithromycin 500 mg on day 1 then 250 mg daily for 4 days (5-day regim...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,meta分析,评审

    doi:10.1136/sextrans-2016-053060

    authors: Horner P,Ingle SM,Garrett F,Blee K,Kong F,Muir P,Moi H

    更新日期:2018-02-01 00:00:00

  • Is the recall of men who have sex with men (MSM) diagnosed as having bacterial sexually transmitted infections (STIs) for re-screening a feasible and effective strategy?

    abstract:OBJECTIVES:To assess the feasibility and outcomes of recalling men who have sex with men (MSM) diagnosed as having a bacterial sexually transmitted infection (STI) for re-screening. METHODS:This evaluation was conducted from December 2008 for a 9-month period. MSM diagnosed as having a bacterial STI in that period wer...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2011-050144

    authors: Harte D,Mercey D,Jarman J,Benn P

    更新日期:2011-12-01 00:00:00

  • Genetic diversity in Trichomonas vaginalis.

    abstract::Recent advances in genetic characterisation of Trichomonas vaginalis isolates show that the extensive clinical variability in trichomoniasis and its disease sequelae are matched by significant genetic diversity in the organism itself, suggesting a connection between the genetic identity of isolates and their clinical ...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,评审

    doi:10.1136/sextrans-2013-051098

    authors: Meade JC,Carlton JM

    更新日期:2013-09-01 00:00:00

  • Self-administered interventions for anogenital warts in immunocompetent patients: a systematic review and meta-analysis.

    abstract:BACKGROUND:Anogenital warts (AGWs, condylomata acuminata) are among the most common STIs and may severely impact quality of life (QoL). Available treatment options are characterised by a high proportion of non-responders and recurrences. OBJECTIVE:To systematically review and meta-analyse the available evidence from r...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,meta分析,评审

    doi:10.1136/sextrans-2016-052768

    authors: Werner RN,Westfechtel L,Dressler C,Nast A

    更新日期:2017-05-01 00:00:00

  • Healthcare provider and service user perspectives on STI risk reduction interventions for young people and MSM in the UK.

    abstract:OBJECTIVE:Behavioural interventions have been shown to reduce sexual behaviours associated with increased risk of sexually transmitted infections in young people (<25 years) and men who have sex with men (MSM) internationally, but evidence from England is limited. We aimed to explore service provider and user experienc...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2018-053903

    authors: Roy A,King C,Gilson R,Richardson D,Burns F,Rodger A,Clark L,Miners A,Pollard A,Desai S,Bailey J,Shahmanesh M,Llewellyn C

    更新日期:2020-02-01 00:00:00

  • First case of "bubonulus" in L2 lymphogranuloma venerum.

    abstract::Since 2003, an ongoing lymphogranuloma venereum (LGV) proctitis outbreak has been reported in industrialised countries with a new variant designated L2b. Only men who have sex with men (MSM) are affected and most are HIV co-infected; delayed or incorrect diagnoses are frequent. We report a rare clinical case of LGV pr...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sti.2007.025395

    authors: Spenatto N,Boulinguez S,de Barbeyrac B,Viraben R

    更新日期:2007-07-01 00:00:00

  • Sex, drugs and smart phone applications: findings from semistructured interviews with men who have sex with men diagnosed with Shigella flexneri 3a in England and Wales.

    abstract:OBJECTIVES:To inform control strategies undertaken as part of an outbreak of Shigella flexneri 3a among men who have sex with men (MSM). METHODS:All men aged ≥18 years diagnosed with S flexneri 3a between October 2012 and May 2013 were invited to participate. Semistructured in-depth quantitative interviews were conduc...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章

    doi:10.1136/sextrans-2015-052014

    authors: Gilbart VL,Simms I,Jenkins C,Furegato M,Gobin M,Oliver I,Hart G,Gill ON,Hughes G

    更新日期:2015-12-01 00:00:00

  • Systematic reviews of point-of-care tests for the diagnosis of urogenital Chlamydia trachomatis infections.

    abstract:BACKGROUND:WHO estimates that 131 million new cases of urogenital Chlamydia trachomatis (CT) infections occur globally every year. Most infections are asymptomatic. Untreated infection in women can lead to severe complications. Screening and treatment of at-risk populations is a priority for prevention and control. OB...

    journal_title:Sexually transmitted infections

    pub_type: 杂志文章,评审

    doi:10.1136/sextrans-2016-053067

    authors: Kelly H,Coltart CEM,Pant Pai N,Klausner JD,Unemo M,Toskin I,Peeling RW

    更新日期:2017-12-01 00:00:00