Abstract:
OBJECTIVES:In the absence of a single nucleic acid amplification test (NAAT) that is both highly specific and sensitive for gonorrhoea, many have put forward the 16S-based assay as a confirmatory test for Neisseria gonorrhoeae. This study was undertaken to evaluate the performance of PCR based on 16S ribosomal gene in comparison with a porA pseudogene-based assay. METHODS:The specificity of both the porA pseudogene-based PCR and 16S ribosomal gene PCR was checked against a panel of strains comprising of non N gonorrhoeae Neisseria sp (NgNS) and other gram-negative and gram-positive bacteria. The sensitivity studies were performed using different dilutions of N gonorrhoeae DNA. PCRs were also done on endocervical and urethral swab samples collected from a total of 100 female and 50 male patients presenting to sexually transmitted disease clinics, Dermatology OPD of AIIMS and Safdarjang Hospital, New Delhi, India, recruited as per inclusion criteria. RESULTS:PCR assay based on 16S ribosomal gene showed cross-reactivity with three of six strains of N sicca. The porA pseudogene-based PCR was highly specific. Analytical sensitivity of 16S-based ribosomal assay was more than that of porA pseudogene-based assay. In clinical samples, for female patients, sensitivity, specificity, positive predictive value and negative predictive value of 16S ribosomal assay was 100% (95% CI 51.7% to 100%), 91.5% (95% CI 83.4% to 96%), 42.9% (95% CI 18.8% to 70.4%) and 100% (95% CI 94.7% to 100%), respectively, while for the male patients it was 100% (95% CI 85% to 100%), 95.5% (95% CI 75.1% to 99.8%), 96.6% (95% CI 80.4% to 99.8%) and 100% (95% CI 80.8% to 100%), respectively. CONCLUSIONS:The data presented in this report supports use of 16S ribosomal assay as a screening assay only. The porA pseudogene target is highly specific for N gonorrhoeae and may be used as a supplemental assay.
journal_name
Sex Transm Infectjournal_title
Sexually transmitted infectionsauthors
Verma R,Sood S,Bala M,Kapil A,Das BK,Sharma VK,Samantaray JCdoi
10.1136/sti.2009.041418subject
Has Abstractpub_date
2010-11-01 00:00:00pages
461-4issue
6eissn
1368-4973issn
1472-3263pii
sti.2009.041418journal_volume
86pub_type
杂志文章abstract:BACKGROUND:In settings with poor sexually transmitted infection (STI) control in high-risk groups, periodic presumptive treatment (PPT) can quickly reduce the prevalence of genital ulcers, Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT). However, few studies have assessed the impact on HIV. Mathematical model...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2008.034678
更新日期:2010-06-01 00:00:00
abstract:OBJECTIVE:To date, the epidemic of HIV infection in Scotland has been primarily associated with injecting drug use. However, the epidemiology of HIV in Scotland changed in the late 1980s, with homosexual men becoming the largest group at risk of HIV infection and AIDS. Our aim was to describe homosexual men's sexual ri...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.75.4.242
更新日期:1999-08-01 00:00:00
abstract:BACKGROUND:Intravaginal practices including vaginal washing have been associated with HIV-1 acquisition. This association may be mediated by mucosal disruption, changes in vaginal flora or genital tract inflammatory responses. Reducing vaginal washing could lower women's risk of HIV-1 acquisition. METHODS:23 HIV-1 ser...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2012-050564
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:HIV is now well established in the Caribbean, with prevalence in several countries being surpassed only by those of sub-Saharan Africa. Continuing inward migration from the Caribbean and a high incidence of some bacterial STIs among Britain's black Caribbean communities, suggests a considerable potential for...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2003.006163
更新日期:2004-02-01 00:00:00
abstract:OBJECTIVES:To review the uptake of a new service for condom provision in the under 16s. METHODS:A retrospective case note review of attenders at an inner London sexual health clinic. RESULTS:The service was audited over a 3 month period in the spring of 2000. There were 97 individual attendances, 94% (92) male and 6%...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.77.4.287
更新日期:2001-08-01 00:00:00
abstract:OBJECTIVE:To assess HIV prevalence and predictive factors for HIV among male sex workers in Spain. METHODS:In this study we analysed all male sex workers who visited HIV testing clinics in 19 Spanish cities between 2000 and 2002. The information was obtained during examination by means of a brief questionnaire. For re...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sti.2003.008649
更新日期:2005-02-01 00:00:00
abstract::In addition to inadequate access to early diagnosis and treatment with antimicrobial agents for patients and sexual contacts, management and control of STIs is significantly challenged by emergence and spread of antimicrobial resistance (AMR), particularly for STIs such as Neisseria gonorrhoeae and Mycoplasma genitali...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2016-053072
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVES:To assess associations between residences location, risky sexual behaviours and sexually transmitted diseases (STDs) among adults living in Guangzhou, China. METHODS:Data were obtained from 751 Chinese adults aged 18-59 years in Guangzhou, China, using stratified random sampling by using spatial epidemiolog...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2015-052268
更新日期:2016-06-01 00:00:00
abstract:BACKGROUND/OBJECTIVES:The polymer conjugate enhanced enzyme immunoassay (IDEIA) and Cobas Amplicor polymerase chain reaction Chlamydia trachomatis (CT) (Amplicor PCR) are two commonly used assays for the diagnosis of CT infection. The performance of these assays was compared for the diagnosis of genital CT infection am...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2005.015362
更新日期:2006-02-01 00:00:00
abstract:OBJECTIVES:To examine the feasibility of non-occupational postexposure prophylaxis (nPEP) as a national strategy for HIV prevention in China, we investigated nPEP usage and related sociodemographic and behavioural factors among five key populations at high risk of contracting HIV. METHODS:We conducted a cross-sectiona...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2020-054791
更新日期:2021-01-04 00:00:00
abstract::The primary objectives of this study were to assess the changing demographic characteristics of female sex workers (FSWs) in the urban Bangalore district, India, and trends in programme coverage, HIV/sexually transmitted infection prevalence rates and condom use. Cross-sectional, integrated behavioural and biological ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2013-051203
更新日期:2013-12-01 00:00:00
abstract::This review will delineate performance characteristics and limitations, as far as they are known, of the new glycoprotein G based, type specific HSV serologies. Several of these tests have been FDA approved in the United States for use in adults. With the departure of Gull/Meridian from the HSV serology market, it is ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sti.77.4.232
更新日期:2001-08-01 00:00:00
abstract:BACKGROUND:Evidence suggests that sexual transmission between men has replaced foreign travel as the predominant mode of Shigella transmission in England. However, sexuality and HIV status are not routinely recorded for laboratory-reported Shigella, and the role of HIV in the Shigella epidemic is not well understood. ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2016-052962
更新日期:2018-02-01 00:00:00
abstract::Azithromycin 1 g immediately and doxycycline 100 mg twice daily have good antimicrobial activity against Chlamydia trachomatis and treatment studies have demonstrated a >95% microbiological cure at 2-5 weeks, with antimicrobial resistance being rarely reported. Recently an 8% (95%, CI 5% to 11%) failure rate was obser...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sti.2005.019158
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:Men who have sex with men (MSM) living with HIV are at high risk of infection with high-risk human papillomavirus (HPV), the cause of anal cancer. We assess whether anal HPV DNA detection is related to recent anal sexual activity, what types of anal sexual activity or the persistence of HPV genotypes. METHO...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,多中心研究
doi:10.1136/sextrans-2015-052121
更新日期:2016-08-01 00:00:00
abstract:OBJECTIVES:To assess sexual risk behaviour and prevalence of treatable sexually transmitted infections (STI) in migrant male workers in Lahore, Pakistan. METHODS:Behavioural interviews were conducted on a representative sample of 590 migrant men aged 20-49 years. Biological samples were collected from a subsample of 1...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2005.018762
更新日期:2006-08-01 00:00:00
abstract:OBJECTIVES:This exploratory study compared the frequency of condom use errors and problems between men reporting that condom use for penile-vaginal sex was a mutual decision compared with men making the decision unilaterally. METHODS:Nearly 2000 people completed a web-based questionnaire. A sub-sample of 660 men repor...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2007.027755
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:Self-tests are performed and interpreted autonomously by a person without involving a healthcare professional or a certified laboratory. To gain insight into the usage of purchased HIV self-tests (HIVST) among migrants living in high-income countries, we studied the prevalence and determinants of HIVST usage...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2018-053583
更新日期:2019-12-01 00:00:00
abstract:BACKGROUND/OBJECTIVES:A growing body of evidence is increasingly demonstrating the effectiveness of condoms for sexually transmitted infection (STI) prevention. The purpose of the present analysis was to provide a disease specific estimate for the effectiveness of condoms in preventing Chlamydia trachomatis infection w...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2004.012799
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVES:To systematically review the evidence of the relation between smoking tobacco and HIV seroconversion and progression to AIDS. METHODS:A systematic review was undertaken of studies to look at tobacco smoking as a risk factor for either HIV seroconversion or progression to AIDS. RESULTS:Six studies were iden...
journal_title:Sexually transmitted infections
pub_type: 杂志文章,评审
doi:10.1136/sti.2005.019505
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVE:To identify prevalence and risks factors for syphilis and HIV among rural female sex workers (FSWs) in Cambodia and to describe differences between rural and urban FSWs. METHODS:Interviews and sera were collected from 114 FSWs and tested for HIV using the Serodia-HIV test and positives confirmed with the enz...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.79.4.e2
更新日期:2003-08-01 00:00:00
abstract:OBJECTIVES:To assess whether a previously established low sexually transmitted infection/HIV risk in prostitutes in London has been sustained, and to measure other occupational risks, including mortality. DESIGN:9 year prospective study in west London. SUBJECTS:402 prostitutes recruited from 1985 to 1991, 320 were fo...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.75.5.340
更新日期:1999-10-01 00:00:00
abstract::Since 2003, a lymphogranuloma venereum epidemic has been reported in The Netherlands and other European countries. This epidemic is caused by Chlamydia trachomatis serovariant L2b and has only been seen in men having sex with men. The authors investigated a woman presenting with a bubo in her right groin. The authors ...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sextrans-2011-050298
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVE:To investigate the possible involvement of human trichomonads (Pentatrichomonas hominis and Trichomonas tenax) other than Trichomonas vaginalis in the aetiology of vaginal trichomoniasis. METHODS:Vaginal swabs taken from women attending antenatal clinics were tested for Trichomonas vaginalis by traditional a...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2006.020941
更新日期:2007-04-01 00:00:00
abstract::Forces at work are described which encourage professionalisation and a reliance on one on one HIV prevention interventions among gay men. Community involvement is intrinsically linked to epidemic phases; when the threat diminishes, so does the community's ability to sustain community level interventions. The area of s...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.78.suppl_1.i176
更新日期:2002-04-01 00:00:00
abstract:OBJECTIVES:There is a dearth of published information on the characteristics of sex workers in Pakistan. This study sought to characterise and compare hijra and non-hijra sex workers from eight large cities in Pakistan. DESIGN:χ(2) and Kruskal-Wallis tests, and multivariable logistic regression were used where appropr...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2009.041335
更新日期:2011-02-01 00:00:00
abstract::The potential for exposure of low and high risk women to HIV and sexually transmitted infections (STI) through unprotected sex with male clients of female sex workers in Cotonou could account for most if not all of the estimated yearly numbers of HIV infections in Cotonou women (approximately 1000). As ongoing transmi...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.78.suppl_1.i69
更新日期:2002-04-01 00:00:00
abstract:OBJECTIVE:To determine the association between oral contraceptive and condom use, and laboratory-confirmed sexually transmitted infection (STI) among African-American adolescent females at a high risk of STI acquisition. METHODS:A cross-sectional study of 715 African-American adolescent females (15-21 years old) was c...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2006.022442
更新日期:2007-06-01 00:00:00
abstract:OBJECTIVES:To measure prevalence of selected sexually transmitted infections (STI) and HIV among female sex workers (SWs) in the red light area of Surat, India, and to evaluate the performance of STI syndrome guidelines (for general population women in India) in this group against the standard aetiological diagnosis of...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.79.2.111
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVES:While much research to date has examined female sex trade work, little has been done to evaluate factors associated with male sex trade involvement or to assess their health service needs. This is particularly true for male sex trade workers who are also injection drug users (IDUs). Therefore, the present an...
journal_title:Sexually transmitted infections
pub_type: 杂志文章
doi:10.1136/sti.2004.011106
更新日期:2004-12-01 00:00:00