Abstract:
:The mammalian target of rapamycin complex 1(mTORC1) integrates diverse signals to control cell growth, proliferation, survival, and metabolism. Role of reactive oxygen species (ROS) on mTORC1 signaling remains obscure and mechanisms through which ROS modulate mTORC1 are not known.We demonstrate that low doses ROS exposure stimulate mTORC1 while high concentrations or long-term ROS treatment decrease mTORC1 activity in vivo and in a variety of cell lines. The dose/time needed for inhibition or activation are cell type dependent. In HEK293 cells hydrogen peroxide (H(2)O(2)) stimulates phosphorylation of AMP-activated kinase (AMPK) (T172) and Raptor (S792), enhances association of activated AMPK with Raptor. Furthermore, AMPK inhibitor compound c inhibits H(2)O(2)-induced Raptor (S792) phosphorylation and reverses H(2)O(2)-induced dephosphorylation of mTORC1 downstream targets p70-S6K1 (T389), S6 (S235/236) and 4E-BP1 (T37/46). H(2)O(2) also stimulates association of endogenous protein phosphatase 2A catalytic subunit (PP2Ac) with p70-S6K1. Like compound c, inhibitor of PP2A, okadaic acid partially reverses inactivation of mTORC1 substrates induced by H(2)O(2). Moreover, inhibition of PP2A and AMPK partially rescued cells from H(2)O(2)-induced cell death. High doses of H(2)O(2) inhibit while low doses of H(2)O(2) activate mTORC1 both in TSC2(-/-) P53(-/-) and TSC2(+/+) P53(-/-) MEFs. These data suggest that PP2A and AMPK-mediated phosphorylation of Raptor mediate H(2)O(2)-induced inhibition of mTORC1 signaling.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Li M,Zhao L,Liu J,Liu A,Jia C,Ma D,Jiang Y,Bai Xdoi
10.1016/j.cellsig.2010.05.015subject
Has Abstractpub_date
2010-10-01 00:00:00pages
1469-76issue
10eissn
0898-6568issn
1873-3913pii
S0898-6568(10)00145-2journal_volume
22pub_type
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