Abstract:
:Impaired switching from fetal haemoglobin (HbF) to adult globin gene expression leads to hereditary persistence of fetal haemoglobin (HPFH) in adult life. This is of prime interest because elevated HbF levels ameliorate β-thalassaemia and sickle cell anaemia. Fetal haemoglobin levels are regulated by complex mechanisms involving factors linked or not to the β-globin gene (HBB) locus. To search for factors putatively involved in the expression of the γ-globin genes (HBG1, HBG2), we examined the reticulocyte transcriptome of three siblings who had different HbF levels and different degrees of β-thalassaemia severity although they had the same ΗBA- and ΗΒB cluster genotypes. By mRNA differential display we isolated the cDNA coding for the cold shock domain protein A (CSDA), also known as dbpA, previously reported to interact in vitro with the HBG2 promoter. Expression studies performed in K562 and in primary erythroid cells showed an inverse relationship between HBG and CSDA expression levels. Functional studies performed by Chromatin Immunoprecipitation and reporter gene assays in K562 cells demonstrated that CSDA is able to bind the HBG2 promoter and suppress its expression. Therefore, our study demonstrated that CSDA is a trans-acting repressor factor of HBG expression and modulates the HPFH phenotype.
journal_name
Br J Haematoljournal_title
British journal of haematologyauthors
Petruzzelli R,Gaudino S,Amendola G,Sessa R,Puzone S,Di Concilio R,d'Urzo G,Amendolara M,Izzo P,Grosso Mdoi
10.1111/j.1365-2141.2010.08303.xsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
689-99issue
6eissn
0007-1048issn
1365-2141pii
BJH8303journal_volume
150pub_type
杂志文章abstract:SUMMARY:We report two patients with chronic myeloid leukaemia (CML) developing hypoplasia and karyotype conversion after conventional busulphan therapy. Initially, the percentage of Ph-positive metaphases in marrow for both patients was 100%, which steadily diminished up to a complete disappearance in case 1 and decrea...
journal_title:British journal of haematology
pub_type: 杂志文章
doi:10.1046/j.1365-2141.1996.431974.x
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journal_title:British journal of haematology
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journal_title:British journal of haematology
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journal_title:British journal of haematology
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pub_type: 杂志文章
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doi:10.1046/j.1365-2141.1996.d01-1903.x
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更新日期:2002-09-01 00:00:00
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 指南,杂志文章,实务指引
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