Sterol metabolism and SREBP activation.

abstract：:The conversion of 25-hydroxyvitamin D3 to 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) takes place mainly in the kidney and is catalyzed by the enzyme 1alpha-hydroxylase. Parathyroid hormone (PTH) and 1,25-(OH)2D3 are well-known regulators of this tightly controlled step, but the mechanisms by which they modulate 1alpha-hy...

journal_title：Archives of biochemistry and biophysics

authors： Brenza HL,DeLuca HF

更新日期：2000-09-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:Alkaline phosphatase (AP) was purified to over 90% homogeneity from rat osteosarcoma by acetone precipitation followed by chromatography on DEAE-cellulose, Sephacryl S-200, and hydroxyapatite. The purified enzyme had a specific activity of 759 units/mg protein at its optimal pH (10.5), and a Km of 0.8 mM for p-nitroph...

journal_title：Archives of biochemistry and biophysics

authors： Nair BC,Majeska RJ,Rodan GA

更新日期：1987-04-01 00:00:00

abstract：:A novel series of nonpeptidic compounds structurally related to the known anticholesteremic thyropropic acid were found to inhibit Escherichia coli peptide deformylase (PDF), with IC50 values in the low-micromolar range. Kinetic analysis of [4-(4-hydroxyphenoxy)-3,5-diiodophenyl]acetic acid reveals competitive inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Jayasekera MM,Kendall A,Shammas R,Dermyer M,Tomala M,Shapiro MA,Holler TP

更新日期：2000-09-15 00:00:00

abstract：:The tandem repeat 23-residue sequence [TRS23 (145-167): T-T-A-A-P-P-T-P-S-A-T-T-P-A-P-P-S-S-S-A-P-P-E] of human salivary mucin glycoprotein MG2 was examined for its in vitro bactericidal activity against four oral microorganisms, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus gordonii, a...

journal_title：Archives of biochemistry and biophysics

authors： Antonyraj KJ,Karunakaran T,Raj PA

更新日期：1998-08-15 00:00:00

abstract：:We have previously shown that rat hepatic cytochromes P450f, P450g, P450h, and P450i possess a high degree of immunochemical and, presumably, structural relatedness. Polyclonal antibodies directed against cytochromes P450f and P450g were made monospecific by immunoabsorption against the cross-reactive proteins. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Bandiera S,Ryan DE,Levin W,Thomas PE

更新日期：1986-08-01 00:00:00

abstract：:Green tea (Camellia sinensis) is rich in catechins, of which (-)-epigallocatechin-3-gallate (EGCG) is the most abundant. Studies in animal models of carcinogenesis have shown that green tea and EGCG can inhibit tumorigenesis during the initiation, promotion and progression stages. Many potential mechanisms have been p...

journal_title：Archives of biochemistry and biophysics

authors： Lambert JD,Elias RJ

更新日期：2010-09-01 00:00:00

abstract：:The structures of the sugar kinase/heat shock 70/actin superfamily of enzymes show that the active site is located in a deep cleft between two domains whose relative movement defines a domain closure conformational change thought to be involved in the catalytic and regulatory properties of members of the superfamily. ...

journal_title：Archives of biochemistry and biophysics

authors： Pettigrew DW,Smith GB,Thomas KP,Dodds DC

更新日期：1998-01-15 00:00:00

abstract：:Since the hepatic LDL receptor is regarded as a major determinant of plasma LDL levels, the effect of diabetes on the expression of this receptor was examined in rat liver. Inducing diabetes with streptozotocin caused a significant reduction in hepatic LDL receptor mRNA levels in concert with an increase in serum chol...

journal_title：Archives of biochemistry and biophysics

authors： Niesen M,Bedi M,Lopez D

更新日期：2008-02-15 00:00:00

abstract：:Biosynthesis of thyroxine in the thyroid gland involves a reaction between two diiodotyrosyl residues within the same molecule of thyroglobulin, a large, thyroid-specific glycoprotein. This reaction, generally referred to as the coupling reaction, is catalyzed in the thyroid by the heme-containing glycoprotein enzyme,...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris M,Doerge DR

更新日期：1994-11-15 00:00:00

abstract：:A recent note from our laboratory reported that L-band (1.2 GHz) electron paramagnetic resonance spectroscopy can be utilized in detecting the formation of Cr(V) intermediates from chromate-treated whole mice. Since Cr(V) is thought to be one of the key species in the mechanism of chromate's toxicity, we carried out a...

journal_title：Archives of biochemistry and biophysics

authors： Liu KJ,Shi X,Jiang JJ,Goda F,Dalal N,Swartz HM

更新日期：1995-10-20 00:00:00

abstract：:The ionophore properties of cationomycin and monensin were studied on human erythrocytes by measuring Na+ influx by 23Na NMR and concomitant K+ efflux by potentiometry in the presence of increasing amounts of serum. Both ion currents (Na+ or K+) decreased linearly with the reciprocal of serum amount. The serum effects...

journal_title：Archives of biochemistry and biophysics

authors： Gibot S,Jeminet G,Juillard J,Gumila C,Ancelin ML,Vial H,Delort AM

更新日期：1999-03-15 00:00:00

abstract：:A catalase-related allene oxide synthase (cAOS) or a hydroperoxide lyase (cHPL) fused together with an 8R-lipoxygenase is involved in the stress signaling of corals via an arachidonic acid pathway. cAOS gives rise to α-ketol and cyclopentenone, while cHPL catalyzes the cleavage of 8R-hydroperoxyeicosatetraenoic acid (...

journal_title：Archives of biochemistry and biophysics

authors： Teder T,Samel N,Lõhelaid H

更新日期：2019-11-15 00:00:00

abstract：:A set of membrane proteins from murine myelomonocytic leukemia cell line WEHI-3BD+ was found to be ADP-ribosylated. Both this ADP-ribosylation reaction and granulocytic differentiation in response to recombinant G-CSF were inhibited approximately 50% by 2 mM benzamide, suggesting a correlation between these two proces...

journal_title：Archives of biochemistry and biophysics

authors： Hanson K,Aboul-Ela N,Jacobson MK,Wu MC

更新日期：1993-04-01 00:00:00

abstract：:Rats fed an ethanol-containing diet for 4 weeks showed a 3- to 5-fold increase over isocalorically pair-fed controls with respect to cytosolic NAD(P)H-quinone oxidoreductase (NQOR) (E.C.1.6.99.2) with both menadione and dichlorophenol-indophenol as substrates. Rates of NAD(P)H-dependent p-nitrosophenol (pNSP) reductio...

journal_title：Archives of biochemistry and biophysics

authors： Hajos AK,Winston GW

更新日期：1992-06-01 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:Atroxase, isolated from the venom of Crotalus atrox (western diamondback rattlesnake), is a nonhemorrhagic protease which has fibrinolytic activity in vivo. The primary structure of atroxase was deduced from the cDNA encoding the atroxase protein. The venom glands of Crotalus atrox were used to prepare a cDNA library....

journal_title：Archives of biochemistry and biophysics

authors： Baker BJ,Wongvibulsin S,Nyborg J,Tu AT

更新日期：1995-03-10 00:00:00

abstract：:Plant polyphenols are extremely diverse, due to the occurrence of several basic structures, numerous substitutions and, for some groups, of polymers (tannins). Plant polyphenol composition depends on the plant species and organ, with some molecules specific of particular plant families while others are ubiquitous. The...

journal_title：Archives of biochemistry and biophysics

authors： Tarascou I,Souquet JM,Mazauric JP,Carrillo S,Coq S,Canon F,Fulcrand H,Cheynier V

更新日期：2010-09-01 00:00:00

abstract：:During the metabolism of 1,2-dimethylhydrazine (a colon carcinogen) in rats, methyl radicals (.CH3) are produced. To gain an insight into the nature of .CH3 interactions with biomolecules in vivo, we conducted in vitro studies using RNA as a model compound. RNA was incubated in a system where .CH3 was generated from t...

journal_title：Archives of biochemistry and biophysics

authors： Kang JO,Gallagher KS,Cohen G

更新日期：1993-10-01 00:00:00

abstract：:Glycophorin from human red blood cells was exposed to ozone in aqueous solution. Amino acid analysis of glycophorin exposed to a 10-fold molar excess of ozone showed that the only residue affected was methionine. Both methionine residues of the protein were oxidized to methionine sulfoxide. Exposure of the oxidized pr...

journal_title：Archives of biochemistry and biophysics

authors： Banerjee SK,Mudd JB

更新日期：1992-05-15 00:00:00

abstract：:While molecular adaptation to high temperature has been extensively studied, the effect of hydrostatic pressure on protein structure and enzymatic activity is still poorly understood. We have studied the influence of pressure on both the quaternary structure and enzymatic activity of the dodecameric TET3 peptidase fro...

journal_title：Archives of biochemistry and biophysics

authors： Rosenbaum E,Gabel F,Durá MA,Finet S,Cléry-Barraud C,Masson P,Franzetti B

更新日期：2012-01-15 00:00:00

abstract：:The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces a novel poly(ADP-ribose) polymerase (TiPARP). In this study, the signaling pathway of the induction was analyzed. Induction of TiPARP by TCDD occurs in both hepa1c1c7 cells and C57 mouse liver. Induction is concentration and time dependen...

journal_title：Archives of biochemistry and biophysics

authors： Ma Q

更新日期：2002-08-15 00:00:00

abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

journal_title：Archives of biochemistry and biophysics

authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

更新日期：1990-01-01 00:00:00

abstract：:Inactivation of the von Hippel-Lindau (VHL) tumor suppressor is associated with renal carcinoma, hemangioblastoma and pheochromocytoma. The VHL protein is a component of a ubiquitin ligase complex that ubiquitinates and degrades hypoxia inducible factor-α (HIF-α). Degradation of HIF-α by VHL is proposed to suppress tu...

journal_title：Archives of biochemistry and biophysics

authors： Lai Y,Song M,Hakala K,Weintraub ST,Shiio Y

更新日期：2012-02-15 00:00:00

abstract：:Human plasma glutathione peroxidase was purified to homogeneity and partially sequenced. Overlapping peptide fragments from three endopeptidase digests permitted the determination of one sequence of 32 contiguous amino acids and one sequence of 23 contiguous amino acids. Five additional unique peptide sequences withou...

journal_title：Archives of biochemistry and biophysics

authors： Esworthy RS,Chu FF,Paxton RJ,Akman S,Doroshow JH

更新日期：1991-05-01 00:00:00

abstract：:The use of affinity chromatography on wheat germ agglutinin columns to partially purify detergent extracts of muscarinic acetylcholine receptor from porcine atria is described. The procedure results in a 20-fold purification of the protein. The partially purified protein binds [3H]L-QNB (the L isomer of quinuclidinyl ...

journal_title：Archives of biochemistry and biophysics

authors： Herron GS,Schimerlik MI

更新日期：1984-05-01 00:00:00

abstract：:Three alanine aminotransferases, two minor (AlaAT-1, AlaAT-3) and one major (AlaAT-2), were detected by native gel electrophoresis of leaf extracts from Panicum miliaceum L. AlaAT-2 was purified to homogeneity and a specific polyclonal antibody was raised against it which did not react with the other two forms of the ...

journal_title：Archives of biochemistry and biophysics

authors： Son D,Jo J,Sugiyama T

更新日期：1991-09-01 00:00:00

abstract：:Differentiation of pluripotent and lineage restricted stem cells such as neural stem cells (NSCs) was studied on conducting substrates of various nature without perturbation of the genome with exogenous genetic material or chemical stimuli. Primary mouse adult neural stem cells (NSCs) and P19 pluripotent embryonal (P1...

journal_title：Archives of biochemistry and biophysics

authors： Ostrakhovitch EA,Byers JC,O'Neil KD,Semenikhin OA

更新日期：2012-12-01 00:00:00

abstract：:Oxidative stress in the small intestine can result in altered cell proliferation, migration, and differentiation of villus-crypt cells. Retinoid metabolism is recognized as an important mediator of cellular differentiation in the intestine. This study examined the effect of oxidative stress in retinoid metabolism in a...

journal_title：Archives of biochemistry and biophysics

authors： Prabhu R,Thomas S,Balasubramanian KA

更新日期：2005-02-15 00:00:00

abstract：:Recognition of defined carbohydrate structures by boar sperm was studied on the basis of oligosaccharide structures of porcine zona pellucida glycoproteins so far elucidated. Boar sperm abundantly adhered to fetuin-Sepharose beads, moderately to asialofetuin-Sepharose beads, but not at all to galactosidase (beta1-4-li...

journal_title：Archives of biochemistry and biophysics

authors： Mori E,Yoshitani N,Mori T,Takasaki S

更新日期：2000-02-01 00:00:00