Abstract：
BACKGROUND:Successful introduction of new anticancer agents into the clinic is often hampered by a lack of qualified biomarkers. Studies have been conducted of 17 ELISAs representing a potential panel of pharmacodynamic/predictive biomarkers for drugs targeted to tumour vasculature. METHODS:The fit-for-purpose approach to method validation was used. Stability studies were performed using recombinant proteins in surrogate matrices, endogenous analytes in healthy volunteer and cancer patient plasma. The impact of platelet depletion was investigated. RESULTS:Method validation focused on measuring precision and showed that 15 of the 17 assays were within acceptable limits. Stability at -80 degrees C was shown for 3 months with all recombinant proteins in surrogate matrices, whereas under the same conditions instability was observed with KGF in platelet-rich and platelet-depleted plasma, and with PDGF-BB in platelet-depleted plasma from cancer patients. For measurement of extracellular circulating analytes, platelet depletion should be conducted before freezing of plasma to prevent release of PDGF-BB, FGFb and VEGF-A. A protocol was developed to remove >90% platelets from plasma requiring centrifugation at 2000 g for 25 min. CONCLUSIONS:These studies highlight the need for assay validation and crucial assessment of sample handling issues before commencement of biomarker analysis in clinical trials.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Brookes K,Cummings J,Backen A,Greystoke A,Ward T,Jayson GC,Dive Cdoi
10.1038/sj.bjc.6605661subject
Has Abstractpub_date
2010-05-11 00:00:00pages
1524-32issue
10eissn
0007-0920issn
1532-1827pii
6605661journal_volume
102pub_type
杂志文章abstract：:A substantial excess risk of lymphomas and nonmelanoma skin cancer has been demonstrated following organ transplantation. Large sample size and long follow-up time may, however, allow more accurate risk estimates and detailed understanding of long-term cancer risk. The objective of the study was to assess the risk of ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601219
更新日期：2003-10-06 00:00:00
abstract：:We evaluated the therapeutic usefulness of adjuvant chemotherapy in patients with completely resected non-small cell lung cancer (NSCLC). We also examined the relation between DNA ploidy pattern and the response to chemotherapy. A total of 267 patients with NSCLC (pathologically documented stage I, II, or IIIA) underw...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/sj.bjc.6603336
更新日期：2006-10-09 00:00:00
abstract：:Pamidronate belongs to the class of nitrogen-containing bisphosphonates that are potent inhibitors of bone resorption frequently used for the treatment of osteoporosis and cancer-induced osteolysis. The inhibition of osteoclasts' growth has been suggested as the main mechanism of the inhibitory effect of pamidronate o...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600476
更新日期：2002-07-29 00:00:00
abstract：:Detailed data were provided by the Oxford Survey of Childhood Cancer OSCC on deaths from childhood cancer in Britain after irradiation of the fetus during diagnostic radiology of the mother. In each age group at death, 0-5, 6-9 and 10-15 years, excess cancer deaths decreased suddenly for births in and after 1958. A ma...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.1990.249
更新日期：1990-07-01 00:00:00
abstract：:We investigated the relation between head and neck cancer risk and alcohol consumption in the NIH-AARP Diet and Health Study. During 2,203,500 person-years of follow-up, 611 men and 183 women developed head and neck cancer. With moderate drinking (up to one alcoholic drink per day) as the referent group, non-drinkers ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603713
更新日期：2007-05-07 00:00:00
abstract：BACKGROUND:To date, no adjuvant treatment has been shown to have a clear benefit in patients with hepatocellular carcinoma (HCC). In this prospective phase I/IIa study, we evaluated the safety and efficacy of adjuvant dendritic cell (DC) therapy in HCC patients who received primary treatment for HCC. METHODS:Twelve HC...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2015.430
更新日期：2015-12-22 00:00:00
abstract：BACKGROUND:This first-in-human, phase I clinical trial of p28 (NSC745104), a 28-amino-acid fragment of the cupredoxin azurin, investigated the safety, tolerability, pharmacokinetics and preliminary activity of p28 in patients with p53(+) metastatic solid tumours. METHODS:A total of 15 patients were administered p28 i....
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.74
更新日期：2013-03-19 00:00:00
abstract：:Leucocytes are a major component of the tumour microenvironment. Recent studies have indicated that the infiltration and activity of these host cells are regulated by the tumour to promote its survival and progression. Through the production of an array of growth factors, proteases and angiogenic mediators, leucocytes...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/sj.bjc.6601705
更新日期：2004-06-01 00:00:00
abstract：BACKGROUND:This study was done to investigate the long-term event free and overall survival of high-dose chemotherapy followed by autologous stem cell transplantation (ASCT), compared to maintenance chemotherapy (MT). Patterns of recurrences and late sequelae of both arms were analysed. METHODS:A randomised open label...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41416-018-0169-8
更新日期：2018-08-01 00:00:00
abstract：BACKGROUND:Overexpression of ErbB2 receptor in breast cancer is associated with disease progression and poor prognosis. Trastuzumab, the only humanised anti-ErbB2 antibody currently used in breast cancer, has proven to be effective; however, a relevant problem for clinical practice is that a high fraction of breast can...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6605499
更新日期：2010-02-02 00:00:00
abstract：BACKGROUND:We previously showed that activation of the nuclear factor of activated T cells (NFAT)1/Fas ligand (FasL) pathway induces glioma cell death. Lithium (Li) is an inhibitor of glycogen synthase kinase (GSK)-3 that activates NFAT1/FasL signalling. Temozolomide (TMZ) inhibits GSK-3 and activates Fas in tumour pro...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2017.89
更新日期：2017-05-09 00:00:00
abstract：:The topical carcinogenicity to mouse skin of smoke condensates obtained from a tobacco substitute (NSM), alone or in combination with tobacco, has been compared with condensate from tobacco and with acetone, the solvent used. Sixteen different types of cigarette were used to make the condensates, and the age-standardi...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.47
更新日期：1977-03-01 00:00:00
abstract：:Fifteen human glioma cell lines were examined for their sensitivity to 1,3-bis(chloroethyl)-nitrosourea (BCNU, carmustine) and cis-dichlorodiamminoplatinum (cisplatin), the induction of DNA interstrand cross-linking (DNA-ISC) induced by the two agents and cellular O6-alkylguanine alkyltransferase (ATase) activity. Cel...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.87
更新日期：1997-01-01 00:00:00
abstract：:Cells from V79 multicell spheroids must be exposed to approximately 50 times more etoposide than exponentially growing monolayers in order to produce the same amount of cell killing. A part of this difference in sensitivity is readily explained by the decrease in growth fraction of large spheroids, and by the protecti...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1993.97
更新日期：1993-03-01 00:00:00
abstract：:High experienced continuity of care in patients with cancer is associated with lower needs for care, better quality of life and better psychological outcomes. We developed and evaluated an intervention to improve experienced continuity. The intervention, consisted of (1) a 17-item patient-completed continuity assessme...
journal_title：British journal of cancer
pub_type： 杂志文章,随机对照试验
doi：10.1038/sj.bjc.6604836
更新日期：2009-01-27 00:00:00
abstract：:We studied 5555 seminoma patients and 3733 patients with nonseminomatous testicular cancers diagnosed in Southeast England between 1960 and 2004. For both groups survival improved over time: 10-year relative survival increased from 78% in 1960-1969 to 99% in 1990-2004 for seminomas, and from 55 to 95% for nonseminomas...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6603589
更新日期：2007-02-12 00:00:00
abstract：:The novel compound 7(-)[4'-(2-nitroimidazol-l-yl)-butyl]-theophylline (NITP) can be used as an immunologically detectable probe for hypoxic cells. Because of the limited water solubility of NITP, it has been administered dissolved in peanut oil with 10% dimethylsulphoxide (DMSO). A new aqueous formulation has been dev...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.530
更新日期：1995-12-01 00:00:00
abstract：BACKGROUND:Bcl-xL has an important role in the control of cell death through its inhibition of apoptosis. The aim of this study was to investigate the clinicopathological significance of Bcl-xL in upper urinary tract urothelial carcinoma (UTUC) and the therapeutic effect of targeting Bcl-xL protein in urothelial carcin...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.216
更新日期：2013-06-11 00:00:00
abstract：:Functionally active telomerase is affected at various steps including transcriptional and post-transcriptional levels of major telomerase components (hTR and human telomerase reverse transcriptase (hTERT)). We therefore developed a rapid and sensitive method to quantify hTERT and its splicing variants as well as the h...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602546
更新日期：2005-05-23 00:00:00
abstract：:We have studied the actions of C-1311, an imidazoacridinone analogue with potent in vivo antitumour activity, against a human tumour line (HeLa S3), in an examination of the events associated with the lethality of this agent. Continuous exposures (24 h) induced complete G2 arrest, although the concentration range of t...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.550
更新日期：1996-11-01 00:00:00
abstract：:Although cancer of the cervix is traditionally considered not to be responsive to steroid hormones, an in vitro study has reported that the addition of oestrogen increased cellular proliferation in a cervix cancer cell line that was inhibited by progesterone. We investigated whether the reported in vitro effects of oe...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1997.97
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:In 2006, the National Health Service Bowel Cancer Screening Programme in England (NHSBCSP) began offering routine population-based biennial faecal occult blood testing (FOBt) at ages 60-69. There is, however, limited information on how characteristics of individuals affect participation and outcomes of scree...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2015.69
更新日期：2015-04-28 00:00:00
abstract：BACKGROUND:Angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin receptor blockers (ARBs) are commonly used antihypertensives. Recently, these drugs have been associated with a protective effect against pancreatic cancer, but data on this putative association remain limited. Thus, the objective of this study...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2016.375
更新日期：2017-01-03 00:00:00
abstract：:Besides its cardiotoxic effect, doxorubicin also elicits inflammatory effects in vivo. 7-Monohydroxyethylrutoside (monoHER) has recently been used as a protector against doxorubicin-induced cardiotoxicity in vivo. It is not known yet whether monoHER can also protect against doxorubicin-induced inflammatory effects. Th...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601022
更新日期：2003-07-21 00:00:00
abstract：BACKGROUND:Operable non-small cell lung cancer (NSCLC) patients whose tumours have spread to regional or central lymph nodes at the time of diagnosis have dismal prognoses compared with those who have limited disease. The current TNM staging system for NSCLC poorly distinguishes patients with lymph-node metastases who ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2016.92
更新日期：2016-05-10 00:00:00
abstract：:The Second Meeting of the British Society for Immunology Tumour Immunology Affinity Group (TIAG) took place at King's College (London, UK) on 17-18 June 1997 and brought together over 100 tumour immunologists from the UK and abroad. In contrast to previous meetings the focus of the meeting was on the role of adhesion ...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.1998.300
更新日期：1998-06-01 00:00:00
abstract：:Tumorigenic mouse lung-derived type 2 cell lines have large reductions in both beta-adrenergic-stimulated cAMP production and ligand binding to beta-adrenergic receptors. These tumorigenic cells are also relatively insensitive to glucocorticoids. Because glucocorticoids regulate both beta-adrenergic receptor expressio...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1996.377
更新日期：1996-08-01 00:00:00
abstract：BACKGROUND:The most significant problem of intra-arterial chemotherapy for advanced paranasal sinus carcinomas and residual cancers supplied by internal carotid artery (ICA) and involving the skull base is the lack of salvage therapies. OBJECTIVE:The objective of the study was to evaluate the usefulness of intra-arter...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.501
更新日期：2014-12-09 00:00:00
abstract：BACKGROUND:The Sloane audit compares screen-detected ductal carcinoma in situ (DCIS) pathology with subsequent management and outcomes. METHODS:This was a national, prospective cohort study of DCIS diagnosed during 2003-2012. RESULTS:Among 11,337 patients, 7204 (64%) had high-grade DCIS. Over time, the proportion of ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/s41416-020-01152-5
更新日期：2020-11-17 00:00:00
abstract：BACKGROUND:Hepatocellular carcinoma (HCC) is one of the most common types of malignant tumour and has poor prognosis. Currently, systematic chemotherapy is the only approach to prolong survival. Thus the development of new treatment regimens is urgently needed to improve the therapeutic efficacy. Our study intended to ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2017.55
更新日期：2017-04-11 00:00:00
