Voluntary exercise enhances survival and migration of neural progenitor cells after intracerebral haemorrhage in mice.

Abstract:

PRIMARY OBJECTIVE:This study explored the long-term effects of exercise on the proliferation, survival and migration of endogenous neural progenitor cells (NPCs) in the subventricular zone (SVZ) of the brain after intracerebral haemorrhage (ICH). RESEARCH DESIGN:ICH was induced by an injection of collagenase into the striatum. Animals in the voluntary running exercise group ran freely on a running wheel for 1, 3 and 6 weeks following the induction of ICH. METHODS AND PROCEDURE:Immunohistochemical labelling was performed to incorporate specific cell markers, such as Ki67 (proliferating cells), 5-bromodeoxyuridien (BrdU; surviving newborn cells) and doublecortin (DCX; neuroblasts or migrating cells). MAIN OUTCOMES AND RESULTS:Voluntary exercise for 3 and 6 weeks sustained more Ki67- or BrdU-immunostained cells in the SVZ after ICH than in the brains of sedentary mice. DCX-immunostained cells were more prominent in the striatum of the group that had exercised for 6 weeks compared to the time-matched sedentary group. Moreover, it was observed that proliferating green fluorescent protein (GFP)-positive cells that were infected with retrovirus were located more distally from the injection site in the exercise group than in the sedentary group. CONCLUSIONS:These data indicate that long-term exercise may enhance the proliferation and survival of NPCs and their migration toward injured areas, suggesting that exercise may contribute to neuronal injury recovery in cell-based therapies after ICH.

journal_name

Brain Inj

journal_title

Brain injury

authors

Jin J,Kang HM,Park C

doi

10.3109/02699051003610458

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

533-40

issue

3

eissn

0269-9052

issn

1362-301X

journal_volume

24

pub_type

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