Extensible behavior of titin in the miniswine left ventricle.


BACKGROUND:The sarcomeric protein titin is a molecular spring responsible for passive tension and restoring forces of cardiomyocytes. Extension of titin as a function of sarcomere length (SL) has been studied in rodents, which predominantly express the smaller, stiffer N2B titin isoform. Large mammals coexpress roughly equal proportions of N2B and N2BA titin, the larger, more compliant isoform. We hypothesized that extension of titin in relation to SL differs in large mammals and that this difference is functionally important. METHODS AND RESULTS:We characterized the filling pressure-SL relation in diastolic-arrested miniswine left ventricles. SL was 2.15 to 2.25 mum at a filling pressure of approximately 0 mm Hg and reached a maximum of approximately 2.50 mum with overfilling. In the normal filling pressure range, SL ranged from approximately 2.32 to approximately 2.40 mum. We assessed titin extension as a function of SL using immunoelectron microscopy, which allowed delineation of the behavior of specific spring segments. The major isoform difference was that the N2B-Us segment extended approximately 4-fold more as a function of SL in N2B compared with N2BA titin. Using this segment, we estimated sarcomeric force development with a worm-like chain model and found that N2B develops markedly greater force than N2BA titin. The resulting force with coexpression of N2B and N2BA titin is intermediate. CONCLUSIONS:In light of murine studies showing that operating SLs are shorter than in miniswine, our results indicate that coexpression of the 2 titin isoforms in large mammals allows longer SLs without the development of excessive diastolic tension.






Lewinter MM,Popper J,McNabb M,Nyland L,Bell SB,Granzier H




Has Abstract


2010-02-16 00:00:00














  • Echocardiographic assessment of left ventricular function in aortic valve disease.

    abstract::Echocardiography was used to study left ventricular size and contraction in 128 patients with isolated aortic valve disease -45 patients with aortic stenosis, 25 with mixed aortic valve disease and 58 with aortic regurgitation. Left ventricular measurements included the end-diastolic internal dimension (LVIDd), mural ...


    pub_type: 杂志文章


    authors: McDonald IG

    更新日期:1976-05-01 00:00:00

  • Passage of inhaled particles into the blood circulation in humans.

    abstract:BACKGROUND:Pollution by particulates has been consistently associated with increased cardiovascular morbidity and mortality. However, the mechanisms responsible for these effects are not well-elucidated. METHODS AND RESULTS:To assess to what extent and how rapidly inhaled pollutant particles pass into the systemic cir...


    pub_type: 杂志文章


    authors: Nemmar A,Hoet PH,Vanquickenborne B,Dinsdale D,Thomeer M,Hoylaerts MF,Vanbilloen H,Mortelmans L,Nemery B

    更新日期:2002-01-29 00:00:00

  • Radiofrequency catheter modification of the sinus node for "inappropriate" sinus tachycardia.

    abstract:BACKGROUND:Radiofrequency catheter ablation is the treatment of choice for patients with paroxysmal supraventricular tachycardias refractory to medical therapy. However, in symptomatic patients with inappropriate sinus tachycardia resistant to drug therapy, catheter ablation of the His' bundle with permanent pacemaker ...


    pub_type: 杂志文章


    authors: Lee RJ,Kalman JM,Fitzpatrick AP,Epstein LM,Fisher WG,Olgin JE,Lesh MD,Scheinman MM

    更新日期:1995-11-15 00:00:00

  • Acute hemodynamic effects of captopril in children with a congestive or restrictive cardiomyopathy.

    abstract::The acute hemodynamic effects of captopril were evaluated at cardiac catheterization in 16 children (age, 0.3-18 years) with cardiomyopathy. Twelve children had congestive cardiomyopathy, whereas four had restrictive cardiomyopathy. Hemodynamic measurements were obtained 30 and 60 minutes after the oral administration...


    pub_type: 临床试验,杂志文章


    authors: Bengur AR,Beekman RH,Rocchini AP,Crowley DC,Schork MA,Rosenthal A

    更新日期:1991-02-01 00:00:00

  • Impact of angiographic complete revascularization after drug-eluting stent implantation or coronary artery bypass graft surgery for multivessel coronary artery disease.

    abstract:BACKGROUND:This study sought to evaluate the clinical impact of angiographic complete revascularization (CR) after drug-eluting stent implantation or coronary artery bypass graft surgery for multivessel coronary disease. METHODS AND RESULTS:A total of 1914 consecutive patients with multivessel coronary disease undergo...


    pub_type: 杂志文章


    authors: Kim YH,Park DW,Lee JY,Kim WJ,Yun SC,Ahn JM,Song HG,Oh JH,Park JS,Kang SJ,Lee SW,Lee CW,Park SW,Park SJ

    更新日期:2011-05-31 00:00:00

  • Targeting of apoptotic macrophages and experimental atheroma with radiolabeled annexin V: a technique with potential for noninvasive imaging of vulnerable plaque.

    abstract:BACKGROUND:Apoptosis is common in advanced human atheroma and contributes to plaque instability. Because annexin V has a high affinity for exposed phosphatidylserine on apoptotic cells, radiolabeled annexin V may be used for noninvasive detection of apoptosis in atherosclerotic lesions. METHODS AND RESULTS:Atheroscler...


    pub_type: 杂志文章


    authors: Kolodgie FD,Petrov A,Virmani R,Narula N,Verjans JW,Weber DK,Hartung D,Steinmetz N,Vanderheyden JL,Vannan MA,Gold HK,Reutelingsperger CP,Hofstra L,Narula J

    更新日期:2003-12-23 00:00:00

  • Myocardial perfusion-contraction matching. Implications for coronary heart disease and hibernation.

    abstract::Experimental studies demonstrate that short-term regional perfusion-contraction matching, in which the energy demands of regional myocardial contraction are reduced to match the diminished myocardial substrate supply, occurs during states of low coronary blood flow under resting conditions and during exercise-induced ...


    pub_type: 杂志文章


    authors: Ross J Jr

    更新日期:1991-03-01 00:00:00

  • Real-time magnetic resonance imaging-guided coronary catheterization in swine.

    abstract:BACKGROUND:We tested the hypothesis that real-time magnetic resonance imaging (MRI) can guide coronary artery catheterization in swine via a percutaneous femoral artery approach. METHODS AND RESULTS:In 12 pigs, we accessed femoral arteries percutaneously. We used 6- or 7-French coronary Judkins catheters filled with d...


    pub_type: 杂志文章


    authors: Omary RA,Green JD,Schirf BE,Li Y,Finn JP,Li D

    更新日期:2003-06-03 00:00:00

  • Effects of weight loss and long-term weight maintenance with diets varying in protein and glycemic index on cardiovascular risk factors: the diet, obesity, and genes (DiOGenes) study: a randomized, controlled trial.

    abstract:BACKGROUND:We sought to separately examine the effects of either weight loss or diets varying in protein content and glycemic index without further changes in body weight on cardiovascular risk factors within the Diet, Obesity, and Genes study (DiOGenes). METHODS AND RESULTS:DiOGenes is a pan-European controlled dieta...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Gögebakan O,Kohl A,Osterhoff MA,van Baak MA,Jebb SA,Papadaki A,Martinez JA,Handjieva-Darlenska T,Hlavaty P,Weickert MO,Holst C,Saris WH,Astrup A,Pfeiffer AF,DiOGenes.

    更新日期:2011-12-20 00:00:00

  • Intrapericardial delivery of L-arginine reduces the increased severity of ventricular arrhythmias during sympathetic stimulation in dogs with acute coronary occlusion: nitric oxide modulates sympathetic effects on ventricular electrophysiological properti

    abstract:BACKGROUND:Nitric oxide (NO) modulates autonomic effects on myocardial contractility and sinus and atrioventricular nodal function of the heart. Whether NO influences autonomic actions on ventricular electrophysiological properties and arrhythmogenesis is not known. METHODS AND RESULTS:Four groups consisting of 43 aut...


    pub_type: 杂志文章


    authors: Fei L,Baron AD,Henry DP,Zipes DP

    更新日期:1997-12-02 00:00:00

  • Infarct tissue heterogeneity by magnetic resonance imaging identifies enhanced cardiac arrhythmia susceptibility in patients with left ventricular dysfunction.

    abstract:BACKGROUND:The extent of the peri-infarct zone by magnetic resonance imaging (MRI) has been related to all-cause mortality in patients with coronary artery disease. This relationship may result from arrhythmogenesis in the infarct border. However, the relationship between tissue heterogeneity in the infarct periphery a...


    pub_type: 临床试验,杂志文章


    authors: Schmidt A,Azevedo CF,Cheng A,Gupta SN,Bluemke DA,Foo TK,Gerstenblith G,Weiss RG,Marbán E,Tomaselli GF,Lima JA,Wu KC

    更新日期:2007-04-17 00:00:00

  • De subitaneis mortibus. XV. Hereditary stenosis of the His bundle in Pug dogs.

    abstract::Syncope and sudden death occurs in certain purebred Pug dogs which have been found to have intermittent sinus pauses and paroxysmal second degree heart block on electrocardiographic (ECG) study. We have established a colony of such dogs to study this problem and here report the results of histological examination of t...


    pub_type: 杂志文章


    authors: James TN,Robertson BT,Waldo AL,Branch CE

    更新日期:1975-12-01 00:00:00

  • The effect of acute changes in coronary blood flow on left ventricular end-diastolic wall thickness. An echocardiographic study.

    abstract::The effect of acute alterations in coronary blood flow (CBF) on left ventricular (LV) end-diastolic wall thickness was measured by reflected ultrasound in twelve anesthetized dogs. During five minutes of coronary ligation, wall thickness decreased from a control of 9.0 +/- 0.3 mm to 8.1 +/- 0.3 mm (P less than 0.001);...


    pub_type: 杂志文章


    authors: Gaasch WH,Bernard SA

    更新日期:1977-10-01 00:00:00

  • Noninvasive assessment of cardiac function and ventricular dyssynergy by precordial Q wave mapping in anterior myocardial infarction.

    abstract::To determine whether multiple lead precordial electrocardiographic recordings offer an improved index for noninvasive estimation of left ventricular hemodynamic function and segmental dyssynergy, precordial mapping was performed in patients with anterior myocardial infarction, and the number of pathologic Q waves (gre...


    pub_type: 杂志文章


    authors: Awan NA,Miller RR,Vera Z,Janzen DA,Amsterdam EA,Mason DT

    更新日期:1977-06-01 00:00:00

  • Circulating microemboli in patients after aortic valve replacement with pulmonary autografts and mechanical valve prostheses.

    abstract:BACKGROUND:The pulmonary autograft procedure (Ross) is now considered the gold standard for aortic valve replacement. One of its advantages is the freedom from macroemboli without anticoagulation. Whether this holds true for circulating microemboli, detectable as high-intensity transient Doppler signals (HITS), has not...


    pub_type: 杂志文章


    authors: Nötzold A,Droste DW,Hagedorn G,Berndt S,Kaps M,Graf B,Sievers HH

    更新日期:1997-09-16 00:00:00

  • Slowing Progression of Cardiovascular Calcification With SNF472 in Patients on Hemodialysis: Results of a Randomized Phase 2b Study.

    abstract:BACKGROUND:The high cardiovascular morbidity and mortality in patients with end-stage kidney disease could be partially caused by extensive cardiovascular calcification. SNF472, intravenous myo-inositol hexaphosphate, selectively inhibits the formation and growth of hydroxyapatite. METHODS:This double-blind, placebo-c...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Raggi P,Bellasi A,Bushinsky D,Bover J,Rodriguez M,Ketteler M,Sinha S,Salcedo C,Gillotti K,Padgett C,Garg R,Gold A,Perelló J,Chertow GM

    更新日期:2020-03-03 00:00:00

  • Transcriptomic and proteomic patterns of systemic inflammation in on-pump and off-pump coronary artery bypass grafting.

    abstract:BACKGROUND:Coronary artery bypass grafting (CABG) using cardiopulmonary bypass (CPB) provides controlled operative conditions but induces a whole-body inflammatory response capable of initiating devastating morbidity and mortality. Although technically more demanding, deliberate avoidance of CPB in off-pump surgery att...


    pub_type: 杂志文章


    authors: Tomic V,Russwurm S,Möller E,Claus RA,Blaess M,Brunkhorst F,Bruegel M,Bode K,Bloos F,Wippermann J,Wahlers T,Deigner HP,Thiery J,Reinhart K,Bauer M

    更新日期:2005-11-08 00:00:00

  • The power of play: Innovations in Getting Active Summit 2011: a science panel proceedings report from the American Heart Association.

    abstract:BACKGROUND:To examine the influence active-play video gaming (also referred to as exergaming, exertainment, and active gaming) might have on improving health-related skills, enhancing self-esteem and self-efficacy, promoting social support, and ultimately motivating positive changes in health behaviors, the American He...




    authors: Lieberman DA,Chamberlin B,Medina E Jr,Franklin BA,Sanner BM,Vafiadis DK,Power of Play: Innovations in Getting Active Summit Planning Committee.

    更新日期:2011-05-31 00:00:00

  • Biventricular circulatory support with two miniaturized implantable assist devices.

    abstract:BACKGROUND:Up to 30% of patients with end-stage heart failure experience biventricular failure that requires biventricular mechanical support. For these patients, only bulky extracorporeal or implantable displacement pumps or the total artificial heart have been available to date, which enables only limited quality of ...


    pub_type: 杂志文章


    authors: Krabatsch T,Potapov E,Stepanenko A,Schweiger M,Kukucka M,Huebler M,Hennig E,Hetzer R

    更新日期:2011-09-13 00:00:00

  • Comparison of long-term outcomes of atrial repair of simple transposition with implications for a late arterial switch strategy.

    abstract:BACKGROUND:We report the single-institution, long-term results of 358 patients with simple transposition of the great arteries surviving >30 days after a Mustard (n=226, 1965 to 1980) or Senning (n=132, 1978 to 1992) procedure. METHODS AND RESULTS:Outcome measures included late death, reintervention, ECG and ambulator...


    pub_type: 杂志文章


    authors: Sarkar D,Bull C,Yates R,Wright D,Cullen S,Gewillig M,Clayton R,Tunstill A,Deanfield J

    更新日期:1999-11-09 00:00:00

  • Increased preferential absorption in human atherosclerotic plaque with oral beta carotene. Implications for laser endarterectomy.

    abstract::Patients undergoing carotid endarterectomy were pretreated with low-dose, oral beta carotene to determine whether the carotenoid content of plaque could be increased in vivo. Beta carotene-treated patients had a 50-fold increase in their plaque beta carotene level from 0.066 to 3.3 micrograms beta carotene/g plaque. M...


    pub_type: 杂志文章


    authors: Prince MR,LaMuraglia GM,MacNichol EF Jr

    更新日期:1988-08-01 00:00:00

  • Cardiac Amyloidosis: Evolving Diagnosis and Management: A Scientific Statement From the American Heart Association.

    abstract::Transthyretin amyloid cardiomyopathy (ATTR-CM) results in a restrictive cardiomyopathy caused by extracellular deposition of transthyretin, normally involved in the transportation of the hormone thyroxine and retinol-binding protein, in the myocardium. Enthusiasm about ATTR-CM has grown as a result of 3 simultaneous a...


    pub_type: 杂志文章


    authors: Kittleson MM,Maurer MS,Ambardekar AV,Bullock-Palmer RP,Chang PP,Eisen HJ,Nair AP,Nativi-Nicolau J,Ruberg FL,American Heart Association Heart Failure and Transplantation Committee of the Council on Clinical Cardiology.

    更新日期:2020-07-07 00:00:00

  • Blacks in the Coronary Artery Surgery Study: risk factors and coronary artery disease.

    abstract::In this paper we examine the relationship between risk factors and angiographically determined coronary artery disease for blacks and whites enrolled in the Coronary Artery Surgery Study (CASS). Analysis of data from the CASS registry indicated that blacks had a higher incidence of hypertension and current cigarette s...


    pub_type: 杂志文章


    authors: Maynard C,Fisher LD,Passamani ER,Pullum T

    更新日期:1986-07-01 00:00:00

  • Regional ischemic 'preconditioning' protects remote virgin myocardium from subsequent sustained coronary occlusion.

    abstract:BACKGROUND:One or more brief episodes of coronary artery occlusion protect or "precondition" the myocardium perfused by that artery from a subsequent episode of sustained ischemia. We sought to determine whether ischemic preconditioning protects only those myocytes subjected to brief coronary occlusion or whether brief...


    pub_type: 杂志文章


    authors: Przyklenk K,Bauer B,Ovize M,Kloner RA,Whittaker P

    更新日期:1993-03-01 00:00:00

  • Prognostic importance of quantitative exercise Doppler echocardiography in asymptomatic valvular aortic stenosis.

    abstract:BACKGROUND:In patients with asymptomatic valvular aortic stenosis, exercise testing may help to stratify the clinical risk. However, data are limited, and the role of quantitative exercise Doppler echocardiography has never been investigated. METHODS AND RESULTS:Sixty-nine consecutive patients with severe asymptomatic...


    pub_type: 杂志文章


    authors: Lancellotti P,Lebois F,Simon M,Tombeux C,Chauvel C,Pierard LA

    更新日期:2005-08-30 00:00:00

  • Improvement of regional myocardial metabolism after coronary thrombolysis induced with tissue-type plasminogen activator or streptokinase.

    abstract::To assess the effects on the heart itself of coronary thrombolysis induced with either tissue-type plasminogen activator (t-PA) or streptokinase (SK), we performed positron emission tomography with 11C-palmitate in 19 patients with initial transmural myocardial infarction immediately after admission and again within 4...


    pub_type: 杂志文章


    authors: Sobel BE,Geltman EM,Tiefenbrunn AJ,Jaffe AS,Spadaro JJ Jr,Ter-Pogossian MM,Collen D,Ludbrook PA

    更新日期:1984-05-01 00:00:00

  • Effects of diastolic transseptal pressure gradient on ventricular septal position and motion.

    abstract::Despite the clinical prevalence of paradoxic interventricular septal (IVS) motion, its pathogenesis remains unclear. To assess the influence of the end-diastolic transseptal pressure gradient, we studied eight open-chest dogs during right ventricular (RV) volume loading (induced by opening a Dacron shunt between the p...


    pub_type: 杂志文章


    authors: Kingma I,Tyberg JV,Smith ER

    更新日期:1983-12-01 00:00:00

  • Preliminary clinical studies with thromboxane synthase inhibitors and thromboxane receptor blockers. A review.

    abstract::This review of the clinical studies of thromboxane synthase inhibitors (TXSIs) and thromboxane receptor blocking drugs (TXRBs) covers the years 1981 to the present. Clinical studies on TXSIs include those in normal volunteers as well as those in patients with angina, peripheral vascular disease and Raynaud's syndrome,...


    pub_type: 杂志文章,评审


    authors: Fiddler GI,Lumley P

    更新日期:1990-01-01 00:00:00

  • Efficacy and safety of varenicline for smoking cessation in patients with cardiovascular disease: a randomized trial.

    abstract:BACKGROUND:Smoking cessation is a key component of secondary cardiovascular disease prevention. Varenicline, a partial alpha4beta2 nicotinic acetylcholine receptor agonist, is effective for smoking cessation in healthy smokers, but its efficacy and safety in smokers with cardiovascular disease are unknown. METHODS AND...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Rigotti NA,Pipe AL,Benowitz NL,Arteaga C,Garza D,Tonstad S

    更新日期:2010-01-19 00:00:00

  • Comparative effects of nitroglycerin, nifedipine and metoprolol on regional left ventricular function in patients with one-vessel coronary disease.

    abstract::To compare acute effects of nitroglycerin (0.8 mg sublingually), nifedipine (5 ng/kg/min i.v.) and metoprolol (0.15 mg/kg i.v.) on normal, ischemic and scarred myocardial segments in man, we performed simultaneous hemodynamic and radionuclide measurements of left ventricular functions. Sixteen patients with isolated l...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Pfisterer M,Glaus L,Burkart F

    更新日期:1983-02-01 00:00:00