Proteomics approaches to understand protein phosphorylation in pathway modulation.

Abstract:

:Signaling pathways in all organisms consist of series of phosphorylation and dephosphorylation events that define directionality and allow different levels of feedback-regulation. Mass spectrometry-based proteomic analyses in recent years have led to a proteome-wide identification of thousands of phosphorylation sites in various plant species. Given this magnitude of mostly qualitative information about protein phosphorylation, discovery of specific phosphoproteins with regulatory functions represents a major challenge. In future large-scale experiments, combinations of data-driven modeling strategies based on quantitative data, targeted kinase-substrate screens, and verification in biochemical and genetic experiments are required to specifically spot phosphorylation sites with specific roles in signaling pathway modulation.

journal_name

Curr Opin Plant Biol

authors

Schulze WX

doi

10.1016/j.pbi.2009.12.008

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

280-87

issue

3

eissn

1369-5266

issn

1879-0356

pii

S1369-5266(10)00002-6

journal_volume

13

pub_type

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