Arterial spin labeling demonstrates that focal amygdalar glutamatergic agonist infusion leads to rapid diffuse cerebral activation.

Abstract:

OBJECTIVES:To investigate acute effects of intra-amygdalar excitatory amino acid administration on blood flow, relaxation time and apparent diffusion coefficient in rat brain. MATERIALS AND METHODS:Several days after MR-compatible cannula placement in right basolateral amygdala, anesthetized rats were imaged at 7 T. Relative cerebral blood flow (CBF) was measured before and 60 min after infusion of 10 nmol KA, cAMPA, ATPA, or normal saline using arterial spin labeling. Quantitative T(2) and diffusion-weighted images were acquired. rCBF, T(2) and ADC values were evaluated in bilateral basolateral amygdala, hippocampus, basal ganglia, frontal and parietal regions. RESULTS:KA led to the highest, and ATPA lowest bilateral rCBF increases. Time courses varied among drugs. T(2) for KA and AMPA was higher while ADC was lower for KA. CONCLUSIONS:Intra-amygdalar injection of GluR agonists evoked bilateral seizure activity and increased rCBF, greater for KA and AMPA than selective ATPA GluR5 activation.

journal_name

Acta Neurol Scand

authors

Munasinghe JP,Banerjee M,Acosta MT,Banks M,Heffer A,Silva AC,Koretsky A,Theodore WH

doi

10.1111/j.1600-0404.2009.01188.x

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

209-16

issue

3

eissn

0001-6314

issn

1600-0404

pii

ANE1188

journal_volume

121

pub_type

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