当前位置： SCI文献检索 > AMERICAN JOURNAL OF NEPHROLOGY期刊下所有文献 > Aldosterone promotes fibronectin synthesis in rat mesangial cells via ERK1/2-stimulated Na-H+ exchanger isoform 1.

Aldosterone promotes fibronectin synthesis in rat mesangial cells via ERK1/2-stimulated Na-H+ exchanger isoform 1.

Abstract：

BACKGROUND:Aldosterone plays an important role in fibrosis. Recent studies showed that Na(+)-H(+) exchanger isoform 1 (NHE1) is involved in mineralocorticoid-induced organ fibrosis. In this study, we examined the role of NHE1 in aldosterone-induced fibronectin accumulation in rat mesangial cells and the signaling pathway involved. METHODS:We detected the expression of mineralocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 2 in rat glomeruli by immunochemistry and Western blot. Then the eukaryotic vectors of shRNA with insert targeting on NHE1 were successfully constructed and transfected into rat mesangial cells. The mRNA was quantified by real-time PCR. We measured the protein abundance of NHE1, ERK1/2 and phosphor-ERK1/2 by Western blot and the level of fibronectin by ELISA. RESULTS:First we demonstrated the local action of aldosterone on rat glomeruli in vivo. Then, after exposure to aldosterone, the NHE1 protein abundance was found increased in rat mesangial cells. Aldosterone treatment markedly increased the fibronectin level, which can be reduced by PD98059, spironolactone and shRNA-NHE1. PD98059 substantially reduced the aldosterone-induced NHE1 expression, while the knocking down of NHE1 did not alter aldosterone-stimulated phospho-ERK1/2 stimulation. CONCLUSION:The present study suggests that NHE1 may play an important role in aldosterone-mediated fibronectin accumulation in rat mesangial cells via the ERK1/2 pathway.

journal_name

Am J Nephrol

journal_title

American journal of nephrology

authors

Zhang M,Chen J,Lai L,You L,Lin S,Hao C,Gu Y

doi

10.1159/000256665

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

75-82

issue

1

eissn

0250-8095

issn

1421-9670

pii

000256665

journal_volume

31

pub_type

杂志文章

相关文献

AMERICAN JOURNAL OF NEPHROLOGY文献大全
  • Thymoglobulin and its use in renal transplantation: a review.

    abstract：:Thymoglobulin (Thymoglobulin®; Genzyme, Cambridge, Mass., USA) is a polyclonal antibody which has been used in the field of transplantation over the last four decades. With an initial hesitancy, it is widely used now in the prevention and treatment of rejection following renal transplantation. Thymoglobulin's lack of ...

    journal_title：American journal of nephrology

    pub_type： 杂志文章,评审

    doi：10.1159/000351643

    authors： Thiyagarajan UM,Ponnuswamy A,Bagul A

    更新日期：2013-01-01 00:00:00

  • Percutaneous transcatheter recanalization in the management of acute renal failure due to sudden occlusion of the renal artery to a solitary kidney.

    abstract：:Percutaneous angioplasty was attempted in 5 patients with acute renal failure due to occlusion of the artery to a solitary functioning kidney. Angioplasty was technically successful in all patients. Renal function was completely restored in 3 and renal perfusion improved in 1 patient. In the fifth patient, renal funct...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000167517

    authors： Kadir S,Watson A,Burrow C

    更新日期：1987-01-01 00:00:00

  • Albuminuria Testing by Race and Ethnicity among Patients with Hypertension with and without Diabetes.

    abstract：BACKGROUND:Detection of chronic kidney disease (CKD) with urine albumin-to-creatinine ratio (UACR) among patients with hypertension (HTN) provides an opportunity for early treatment, potentially mitigating risk of CKD progression and cardiovascular complications. Differences in UACR testing patterns among racial/ethnic...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000500706

    authors： Lee J,Chu C,Guzman D,Fontil V,Velasquez A,Powe NR,Tuot DS

    更新日期：2019-01-01 00:00:00

  • Clinical implications of antineutrophil cytoplasmic antibody test in lupus nephritis.

    abstract：:To elucidate the prevalence and clinical implications of antineutrophil cytoplasmic antibody (ANCA) in lupus nephritis (LN), we examined ANCA by indirect immunofluorescence and by ELISA against antilactoferrin (anti-LF) and antimyeloperoxidase (anti-MPO) antibody. To discriminate perinuclear ANCA (pANCA) with antinucl...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000013557

    authors： Chin HJ,Ahn C,Lim CS,Chung HK,Lee JG,Song YW,Lee HS,Han JS,Kim S,Lee JS

    更新日期：2000-01-01 00:00:00

  • Dietary Sodium/Potassium Intake Does Not Affect Cognitive Function or Brain Imaging Indices.

    abstract：BACKGROUND:Dietary sodium may influence cognitive function through its effects on cerebrovascular function and cerebral blood flow. METHODS:The aim of this study was to evaluate the association of dietary sodium intake with cognitive decline in community-dwelling older adults. We also evaluated the associations of die...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000486580

    authors： Nowak KL,Fried L,Jovanovich A,Ix J,Yaffe K,You Z,Chonchol M

    更新日期：2018-01-01 00:00:00

  • The endothelin pathway: a protective or detrimental target of bardoxolone methyl on cardiac function in patients with advanced chronic kidney disease?

    abstract：:Bardoxolone methyl has been reported to cause detrimental cardiovascular events in the terminated BEACON Phase III human clinical trial via modulation of the renal endothelin pathway. However, the effects of bardoxolone methyl administration on the endothelin pathway in the heart are unknown. Our purpose in this persp...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000368563

    authors： Camer D,Huang XF

    更新日期：2014-01-01 00:00:00

  • Rosiglitazone increases PPARgamma in renal tubular epithelial cells and protects against damage by hydrogen peroxide.

    abstract：BACKGROUND/AIMS:Thiazolidinediones (TZD) are ligands known to bind to and activate the nuclear peroxisome proliferator-activated receptor gamma (PPARgamma), and are currently used as insulin sensitizers in type 2 diabetes. Recently, several studies have shown that TZD may have a role in renal protection in various expe...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000105125

    authors： Sommer M,Wolf G

    更新日期：2007-01-01 00:00:00

  • Posttransplant diabetes in kidney transplant recipients.

    abstract：:We retrospectively reviewed the course of 1,000 renal transplants performed in 835 recipients (758 nondiabetics) to assess the incidence of new onset posttransplant diabetes in former nondiabetics. A total of 119 (15.7%) recipients manifested posttransplant diabetes, of whom 64 (53.8%) became hyperglycemic within 3 we...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000166932

    authors： Friedman EA,Shyh TP,Beyer MM,Manis T,Butt KM

    更新日期：1985-01-01 00:00:00

  • Febuxostat ameliorates diabetic renal injury in a streptozotocin-induced diabetic rat model.

    abstract：BACKGROUND:Oxidative stress and inflammation are known to play central roles in the development of diabetic nephropathy (DN). Febuxostat is a novel non-purine xanthine oxidase (XO)-specific inhibitor developed to treat hyperuricemia. In this study, we investigated whether febuxostat could ameliorate DN via renoprotecti...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000363421

    authors： Lee HJ,Jeong KH,Kim YG,Moon JY,Lee SH,Ihm CG,Sung JY,Lee TW

    更新日期：2014-01-01 00:00:00

  • Gut Microbiota Dysbiosis in Children with Relapsing Idiopathic Nephrotic Syndrome.

    abstract：BACKGROUND:While the etiology of idiopathic nephrotic syndrome (idiopathic nephrotic syndrome [INS]; characterized by repeated relapses and comorbid allergic conditions) remains unknown, recent evidence suggests that dysfunction in regulatory T cells (Tregs) plays an important role in the development of INS as well as ...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000487557

    authors： Tsuji S,Suruda C,Hashiyada M,Kimata T,Yamanouchi S,Kitao T,Kino J,Akane A,Kaneko K

    更新日期：2018-01-01 00:00:00

  • Insights from Screening a Racially and Ethnically Diverse Population for Chronic Kidney Disease.

    abstract：BACKGROUND:The value of chronic kidney disease (CKD) screening in the general population remains unclear but may be beneficial in populations with high disease prevalence. We examined risk factors for albuminuria among participants in a state-wide CKD screening program in Hawaii. METHODS:The National Kidney Foundation...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000455389

    authors： Wong LL,Kalantar-Zadeh K,Page V,Hayashida G,You AS,Rhee CM

    更新日期：2017-01-01 00:00:00

  • Peritoneal sclerosis in peritoneal dialysis patients.

    abstract：:Peritoneal sclerosis, a disorder similar to that previously identified in nonuremic patients, is being noted in peritoneal dialysis patients with increasing frequency. The etiology in dialysis patients remains unknown. An association with previous or ongoing peritoneal inflammation or irritation suggests that the inci...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000166799

    authors： Ing TS,Daugirdas JT,Gandhi VC

    更新日期：1984-01-01 00:00:00

  • Continuous venovenous hemodiafiltration in the treatment of acute hyperammonemia.

    abstract：:Acute hyperammonemia is an emergent cause of central nervous system dysfunction for which renal replacement therapy is advocated. We report the successful use of continuous venovenous hemodiafiltration in this metabolic emergency, and report the calculated ammonia clearances for both continuous venovenous hemofiltrati...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000013400

    authors： Braun MC,Welch TR

    更新日期：1998-01-01 00:00:00

  • Early Activation of PINCH/ Glycogen-Synthase Kinase 3β/ERK Pathway in Obstructive Nephropathy Rat Model.

    abstract：BACKGROUND:Despite a previous study showing that PINCH-1 exerts an important role in regulating TGF-β1-mediated mesenchymal transition through its interaction with integrin-linked kinase, relatively little is known about the role of PINCH in the obstructive nephropathy. METHODS:To construct a rat model of renal inters...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000450838

    authors： Jia L,Li X,Gu Y,Cui W

    更新日期：2016-01-01 00:00:00

  • Biochemical abnormalities of platelets in renal failure. Evidence for decreased platelet serotonin, adenosine diphosphate and Mg-dependent adenosine triphosphatase.

    abstract：:The platelet content of adenosine triphosphate (ATP), adenosine diphosphate (ADP), serotonin and ouabain-insensitive, magnesium-dependent adenosine triphosphatase (ATPase) was determined in patients with chronic renal failure, patients on chronic hemodialysis, and kidney transplant recipients. Platelet ATP content was...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000166482

    authors： Eknoyan G,Brown CH 3rd

    更新日期：1981-01-01 00:00:00

  • Relapse of Nephrotic Syndrome after Adrenocorticotropic Hormone-Induced Remission: Implications of Adrenocorticotropic Hormone Antibodies.

    abstract：BACKGROUND:Prolonged use of corticosteroids continues to be the mainstay in the management of most proteinuric glomerulopathies, but is limited by extensive side effects. Alternative medications such as adrenocorticotropic hormone (ACTH) have been recently used to treat refractory glomerulopathies and have shown superi...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000506854

    authors： Shrivastava S,Chen B,Dworkin LD,Malhotra DK,Gong R

    更新日期：2020-01-01 00:00:00

  • Combination Cyclophosphamide/Glucocorticoids Provide Better Tolerability and Outcomes versus Glucocorticoids Alone in Patients with Sjogren's Associated Chronic Interstitial Nephritis.

    abstract：BACKGROUND:Steroid therapy has become an effective option for patients with primary Sjogren's syndrome with tubulointerstitial nephritis (TIN), while the use of cytotoxic agents is still debated. Our study aimed to compare the clinical outcomes of patients treated with cyclophosphamide (CTX) combined with glucocorticoi...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000484903

    authors： Shen Y,Xie J,Lin L,Li X,Shen P,Pan X,Ren H,Chen N

    更新日期：2017-01-01 00:00:00

  • Creatinine clearance but not serum creatinine alone predicts long-term postoperative survival after lower extremity revascularization.

    abstract：BACKGROUND:Renal insufficiency is a well-described risk factor for perioperative morbidity and shortened survival after major vascular procedures. Due to the potential inaccuracy of serum creatinine levels alone in measuring kidney function, our aim was to determine whether estimated creatinine clearance more consisten...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000098150

    authors： Maithel SK,Pomposelli FB,Williams M,Sheahan MG,Scovell SD,Campbell DR,LoGerfo FW,Hamdan AD

    更新日期：2006-01-01 00:00:00

  • Uric acid: a novel risk factor for acute kidney injury in high-risk cardiac surgery patients?

    abstract：BACKGROUND:Uric acid has been reported to be a risk factor for the development of chronic kidney disease; however, no study has examined whether uric acid may confer a risk for acute kidney injury. METHODS:We investigated the relation between serum uric acid and the incidence of postoperative acute kidney injury in pa...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000238824

    authors： Ejaz AA,Beaver TM,Shimada M,Sood P,Lingegowda V,Schold JD,Kim T,Johnson RJ

    更新日期：2009-01-01 00:00:00

  • A comparison of plasma and muscle carnitine levels in patients on peritoneal or hemodialysis for chronic renal failure.

    abstract：:We studied plasma, dialysate, and muscle carnitine levels in patients with stable chronic renal failure on hemodialysis, and intermittent peritoneal, or continuous ambulatory peritoneal dialysis (CAPD). In patients on hemodialysis, plasma carnitine levels fell from 46.2 +/- 4.5 mumol/l (mean +/- SEM) to 18.8 +/- 2.7 m...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000166711

    authors： Moorthy AV,Rosenblum M,Rajaram R,Shug AL

    更新日期：1983-07-01 00:00:00

  • Impaired vascular reactivity in patients with chronic kidney disease.

    abstract：BACKGROUND:Patients with chronic kidney disease (CKD) show increased cardiovascular morbidity. We hypothesized that vascular properties which can be routinely evaluated noninvasively are related to different stages of CKD and their clinical and biochemical characteristics. METHODS:Arterial vascular properties were qua...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000110091

    authors： Tetzner F,Scholze A,Wittstock A,Zidek W,Tepel M

    更新日期：2008-01-01 00:00:00

  • Elevated preoperative phosphorus levels are an independent risk factor for cardiovascular mortality.

    abstract：BACKGROUND/AIMS:Serum phosphorus levels have been associated with adverse long-term outcome in several populations, however, no prior studies evaluated short-term postoperative outcome. The present study evaluated the predictive value of phosphorus levels on 30-day outcome after vascular surgery. METHODS:The study inc...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000315856

    authors： van Kuijk JP,Flu WJ,Chonchol M,Valentijn TM,Verhagen HJ,Bax JJ,Poldermans D

    更新日期：2010-01-01 00:00:00

  • Vitalism and synthesis of urea. From Friedrich Wöhler to Hans A. Krebs.

    abstract：:In 1828, Friedrich Wöhler, a German physician and chemist by training, published a paper that describes the formation of urea, known since 1773 to be a major component of mammalian urine, by combining cyanic acid and ammonium in vitro. In these experiments the synthesis of an organic compound from two inorganic molecu...

    journal_title：American journal of nephrology

    pub_type： 传,历史文章,杂志文章

    doi：10.1159/000013463

    authors： Kinne-Saffran E,Kinne RK

    更新日期：1999-01-01 00:00:00

  • Maimonides: physician and nephrologist.

    abstract：:Maimonides (Moses ben Maimon) was born in 1135 in Cordoba, the son of a Jewish rabbi. After a seminomadic upbringing in Spain and North Africa during the rule of the Almohades, Maimonides settled in Fostate (Old Cairo), where he became renowned as a physician, eventually being appointed as court physician to Saladin a...

    journal_title：American journal of nephrology

    pub_type： 传,历史文章,杂志文章

    doi：10.1159/000168739

    authors： Massry SG

    更新日期：1994-01-01 00:00:00

  • Tacrolimus improves the proteinuria remission in patients with refractory IgA nephropathy.

    abstract：BACKGROUND:Tacrolimus has been reported to be effective in refractory nephrotic syndrome, such as focal segmental glomerulosclerosis and membranous nephropathy. Some IgA nephropathy (IgAN) patients with massive proteinuria showed resistance to steroids and/or cytotoxic immunosuppressants based on the supportive therapy...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000337175

    authors： Zhang Q,Shi SF,Zhu L,Lv JC,Liu LJ,Chen YQ,Zhang H,Wang HY

    更新日期：2012-01-01 00:00:00

  • Adverse mandibular bone effects associated with kidney disease are only partially corrected with bisphosphonate and/or calcium treatment.

    abstract：BACKGROUND/AIMS:Patients with chronic kidney disease (CKD) have a high prevalence of periodontal disease that may predispose to tooth loss and inflammation. The goal of this study was to test the hypotheses that a genetic rat model of progressive CKD would exhibit altered oral bone properties and that treatment with ei...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000356335

    authors： Allen MR,Chen NX,Gattone VH 2nd,Moe SM

    更新日期：2013-01-01 00:00:00

  • Impact of Serum Bicarbonate Levels on Muscle Mass and Kidney Function in Pre-Dialysis Chronic Kidney Disease Patients.

    abstract：BACKGROUND:Treatment of metabolic acidosis to target the higher serum bicarbonate level than guideline recommendation may downregulate muscle protein degradation and improve renal function among chronic kidney disease (CKD) patients. We conducted a study to test the effects of increased serum bicarbonate level on muscl...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000504557

    authors： Kittiskulnam P,Srijaruneruang S,Chulakadabba A,Thokanit NS,Praditpornsilpa K,Tungsanga K,Eiam-Ong S

    更新日期：2020-01-01 00:00:00

  • Association of prohepcidin and hepcidin-25 with erythropoietin response and ferritin in hemodialysis patients.

    abstract：:Hepcidin is a key regulator of iron metabolism. In this study, we examined whether measurement of hepcidin is useful in assessing recombinant human erythropoietin (rHuEPO) responsiveness in regular hemodialysis (HD) patients in a cross-sectional fashion. We examined the association between serum prohepcidin, a prohorm...

    journal_title：American journal of nephrology

    pub_type： 临床试验,杂志文章

    doi：10.1159/000109968

    authors： Kato A,Tsuji T,Luo J,Sakao Y,Yasuda H,Hishida A

    更新日期：2008-01-01 00:00:00

  • Albuminuria Regression and All-Cause Mortality among Insulin-Treated Patients with Type 2 Diabetes: Analysis of a Large UK Primary Care Cohort.

    abstract：BACKGROUND:Overt albuminuria (urinary albumin-creatinine ratio [ACR] > 300 mg/g) is an established risk factor for progression of nephropathy and total mortality. However, whether a reduction in ACR translates into a reduction in mortality and/or cardiovascular (CV) events among insulin-treated patients with type 2 dia...

    journal_title：American journal of nephrology

    pub_type： 杂志文章

    doi：10.1159/000496276

    authors： Anyanwagu U,Donnelly R,Idris I

    更新日期：2019-01-01 00:00:00

  • Dialyzer reuse and mortality risk in patients with end-stage renal disease: a systematic review.

    abstract：BACKGROUND AND AIM:Robust evidence about dialyzer reuse effects on mortality is not available. Our aim was to summarize the evidence for the effectiveness of dialyzer reuse compared to single use in patients with end-stage renal disease. METHODS:We searched MEDLINE, Embase, CINAHL, SciELO, LILACS, USRDS ADR, universit...

    journal_title：American journal of nephrology

    pub_type： 杂志文章,评审

    doi：10.1159/000336532

    authors： Galvao TF,Silva MT,Araujo ME,Bulbol WS,Cardoso AL

    更新日期：2012-01-01 00:00:00