Abstract:
:Human parvovirus B19 (B19V) infection shows a strong erythroid tropism and drastically destroys erythroid progenitor cells, thus leading to most of the disease outcomes associated with B19V infection. In this study, we systematically examined the 3 B19V nonstructural proteins, 7.5 kDa, 11 kDa, and NS1, for their function in inducing apoptosis in transfection of primary ex vivo-expanded erythroid progenitor cells, in comparison with apoptosis induced during B19V infection. Our results show that 11 kDa is a more significant inducer of apoptosis than NS1, whereas 7.5 kDa does not induce apoptosis. Furthermore, we determined that caspase-10, an initiator caspase in death receptor signaling, is the most active caspase in apoptotic erythroid progenitors induced by 11 kDa and NS1 as well as during B19V infection. More importantly, cytoplasm-localized 11 kDa is expressed at least 100 times more than nucleus-localized NS1 at the protein level in primary erythroid progenitor cells infected with B19V; and inhibition of 11 kDa expression using antisense oligos targeting specifically to the 11 kDa-encoding mRNAs reduces apoptosis significantly during B19V infection of erythroid progenitor cells. Taken together, these results demonstrate that the 11 kDa protein contributes to erythroid progenitor cell death during B19V infection.
journal_name
Bloodjournal_title
Bloodauthors
Chen AY,Zhang EY,Guan W,Cheng F,Kleiboeker S,Yankee TM,Qiu Jdoi
10.1182/blood-2009-04-215756subject
Has Abstractpub_date
2010-02-04 00:00:00pages
1070-80issue
5eissn
0006-4971issn
1528-0020pii
blood-2009-04-215756journal_volume
115pub_type
杂志文章相关文献
BLOOD文献大全abstract::Macrophage colony-stimulating factor (M-CSF or CSF-1) and granulocyte-macrophage CSF (GM-CSF) have been shown to increase human monocyte urokinase-type plasminogen-activator (u-PA) activity with possible consequences for cell migration and tissue remodeling; because monocyte u-PA activity is likely to be controlled in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-12-15 00:00:00
abstract::Burkitt lymphoma (BL) is classified into 3 clinical subsets: endemic, sporadic, and immunodeficiency-associated BL. So far, possible differences in their gene expression profiles (GEPs) have not been investigated. We studied GEPs of BL subtypes, other B-cell lymphomas, and B lymphocytes; first, we found that BL is a u...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-08-301556
更新日期:2011-03-31 00:00:00
abstract::Women treated at young ages with supradiaphragmatic radiotherapy for Hodgkin lymphoma (HL) have a highly increased risk of breast cancer. For personalized advice and follow-up regimens for patients, information is needed on how the radiotherapy-related risk is affected by other breast cancer risk factors. Genome-wide ...
journal_title:Blood
pub_type: 杂志文章,meta分析
doi:10.1182/blood-2011-10-383380
更新日期:2012-01-26 00:00:00
abstract::Gastrointestinal (GI) bleeding is distinctive of severe von Willebrand disease (VWD), generally arising in older patients; in most cases, blood transfusion and hospitalization are required. The presence of arteriovenous malformations is often described when endoscopic examinations are performed. Patients with congenit...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019003702
更新日期:2020-09-03 00:00:00
abstract::A male neonate with Down's syndrome and congenital myeloproliferative disorder was studied. His blood picture showed the unique coexistence of leukocytosis with matured cells and a large number of blast cells. The in vitro proliferation and differentiation of blast cells into various lineages in the presence of phytoh...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-02-01 00:00:00
abstract::Hematopoiesis is tightly controlled by transcription regulatory networks, but how and when specific transcription factors control lineage commitment are still largely unknown. Within the hematopoietic stem cell (Lin(-)Sca-1(+)c-Kit(+)) compartment these lineage-specific transcription factors are expressed at low level...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-03-275404
更新日期:2010-11-18 00:00:00
abstract::Erythropoiesis is a robust process of cellular expansion and maturation occurring in murine bone marrow and spleen. We previously determined that sublethal irradiation, unlike bleeding or hemolysis, depletes almost all marrow and splenic erythroblasts but leaves peripheral erythrocytes intact. To better understand the...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-11-394304
更新日期:2012-09-20 00:00:00
abstract::The level of expression of the enzyme thiopurine methyltransferase (TPMT) is an important determinant of the metabolism of thiopurines used in the treatment of acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Studies in red blood cells (RBC) have shown that TPMT expression displays genetic polymorp...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-10-15 00:00:00
abstract::We have performed a retrospective analysis of the development of T- and B-cell functions after HLA-nonidentical T-cell-depleted bone marrow transplantation (BMT) performed in 193 patients with severe combined immunodeficiency (SCID) at 18 European centers between December 1982 and December 31, 1993. One hundred sixtee...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:
更新日期:1998-05-15 00:00:00
abstract::Hematopoietic stem cell (HSC) homeostasis is controlled by cytokine receptor-mediated Janus kinase 2 (JAK2) signaling. We previously found that JAK2 is promptly ubiquitinated upon cytokine stimulation. Whether a competing JAK2 deubiquitination activity exists is unknown. LNK is an essential adaptor protein that constr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2018-10-877563
更新日期:2019-04-04 00:00:00
abstract::We report on a second generation of transgenic mice produced by crossing a transgenic mouse line expressing high levels of human alpha and beta S chains (alpha H beta S [beta MDD]) with a line expressing human alpha and beta S-Antilles (beta SAnt). We hypothesized that mice expressing both hemoglobins (Hbs) would have...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-09-15 00:00:00
abstract::Mantle cell lymphoma (MCL) is a mature B-cell neoplasm initially driven by CCND1 rearrangement with 2 molecular subtypes, conventional MCL (cMCL) and leukemic non-nodal MCL (nnMCL), that differ in their clinicobiological behavior. To identify the genetic and epigenetic alterations determining this diversity, we used w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020005289
更新日期:2020-09-17 00:00:00
abstract::All-trans retinoic acid (ATRA) is an essential component of the treatment of acute promyelocytic leukemia (APL), but the optimal timing and duration remain to be determined. Molecular characterization of this disease can refine the diagnosis and could be potentially useful in monitoring response to treatment. Patients...
journal_title:Blood
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1999-06-15 00:00:00
abstract::The laboratory of Judah Folkman identified the potent endogenous antiangiogenic protein angiostatin in 1994.(1) In this issue of Blood, Lee and colleagues propose 2 new mechanisms of action for angiostatin that may represent promising targets for new cancer therapeutics.(2). ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2009-06-226233
更新日期:2009-08-27 00:00:00
abstract::Chronic myeloid leukemia (CML) is caused by the acquisition of the tyrosine kinase BCR-ABL1 in a hemopoietic stem cell, transforming it into a leukemic stem cell (LSC) that self-renews, proliferates, and differentiates to give rise to a myeloproliferative disease. Although tyrosine kinase inhibitors (TKIs) that target...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2016-09-696013
更新日期:2017-03-23 00:00:00
abstract::An inverse relationship between BCL-2 expression and cell cycle transition has been suggested by recent studies in murine models. To investigate the clinical relevance of these laboratory studies, a group of 116 paraffin-embedded non-Hodgkin's lymphoma (NHL) biopsy specimens (Working Formulation Groups D-H, and J) fro...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:
更新日期:1998-02-15 00:00:00
abstract::Protein vaccines for T-cell immunity are not being prioritized because of poor immunogenicity. To overcome this hurdle, proteins are being targeted to maturing dendritic cells (DCs) within monoclonal antibodies (mAbs) to DC receptors. To extend the concept to humans, we immunized human immunoglobulin-expressing mice w...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-06-288068
更新日期:2010-11-11 00:00:00
abstract::In animal models, tissue stem cells were proposed to exhibit an unexpected level of plasticity, although issues on cell fusions have lead to some controversies. Only transplantation experiments using genetically distinct recipients and donors can unequivocally show these changes in cell fate. We have analyzed oral squ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-07-171702
更新日期:2009-02-19 00:00:00
abstract::There is now strong experimental evidence that tissue factor pathway inhibitor (TFPI) is a critical inhibitor to modulate tissue factor-induced coagulation, but the role of TFPI as a risk factor for thrombosis is yet to be to be determined. This study investigated the role of low TFPI levels for the development of dee...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3188
更新日期:2003-06-01 00:00:00
abstract::The membrane-anchored CX3C chemokine fractalkine (FKN) is expressed on activated endothelium and is associated with the development of atherosclerosis. The potential of FKN in mediating platelet adhesion beyond platelet activation remains unexplored to date. A flow-based adhesion assay was used to study the adhesion o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-02-335471
更新日期:2011-05-05 00:00:00
abstract::Prolylcarboxypeptidase (PRCP) activates prekallikrein to plasma kallikrein, leading to bradykinin liberation, and degrades angiotensin II. We now identify PRCP as a regulator of blood vessel homeostasis. β-Galactosidase staining in PRCP(gt/gt) mice reveals expression in kidney and vasculature. Invasive telemetric moni...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-11-318527
更新日期:2011-04-07 00:00:00
abstract::Although both P- and E-selectin are constitutively expressed on bone marrow endothelial cells, their role in the regulation of hematopoiesis has only recently been investigated. We have previously shown that P-selectin glycoprotein ligand-l (PSGL-1/CD162) is expressed by primitive human bone marrow CD34+ cells, mediat...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-06-1921
更新日期:2004-03-01 00:00:00
abstract::Plasminogen activation rates are enhanced by cell surface binding. We previously demonstrated that exogenous plasminogen binds to phosphatidylserine-exposing and spread platelets. Platelets contain plasminogen in their α-granules, but secretion of plasminogen from platelets has not been studied. Recently, a novel tran...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020007263
更新日期:2021-01-14 00:00:00
abstract::Positron emission tomography (PET) using F-18 fluorodeoxyglucose (FDG) was performed in non-Hodgkin's lymphoma (NHL), which is known to be highly responsive to chemotherapy, but also yields variable treatment results to answer the following questions: (1) What is the extent and time course of changes in FDG utilizatio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-06-15 00:00:00
abstract::To elucidate the role of thrombocytosis, alone or in combination with standard (age, previous cardiovascular events) and novel (leukocytosis, JAK2(V617F) mutational status) risk factors, in the cardiovascular events of essential thrombocythemia (ET), we analyzed a cohort of 1063 patients. We found that a platelet coun...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-04-153783
更新日期:2008-10-15 00:00:00
abstract::Pharmaceutical preparations of normal human immunoglobulin (IgG) are known to contain high-avidity and neutralizing antibodies (Ab) to the cytokines interleukin (IL)-1alpha, IL-6, and interferon (IFN)alpha. To test for other cytokine Ab, 23 batches of IgG were tested for saturable binding to eight 125I-labeled recombi...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-03-15 00:00:00
abstract::Macrophages infiltrating solid tumors exhibit a tumor-supporting phenotype and are critical for tumor development. Little is known which tumor-derived signal provokes this phenotype shift and how these signals are interpreted in macrophages to support tumor growth. We used the supernatant of apoptotic cells and notice...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-01-201889
更新日期:2009-09-03 00:00:00
abstract::Human erythroid alpha-spectrin alleles responsible for hereditary elliptocytosis (alphaHE alleles) undergo increased incorporation into red blood cell membranes when the polymorphism alphaLELY (LELY: Low Expression LYon) occurs in trans. The alphaLELY polymorphism is characterized by a mutation in exon 40 at codon 185...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-11-15 00:00:00
abstract::Unfractionated heparin (UFH) is a widely used anticoagulant that has long been known to potentiate platelet responses to subthreshold doses of platelet agonists. UFH has been reported to bind and induce modest conformational changes in the major platelet integrin, αIIbβ3, and induce minor changes in platelet morpholog...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-09-307751
更新日期:2011-05-05 00:00:00
abstract::We analyzed factors having an impact on response to treatment and survival in 78 consecutive patients with chronic myeloid leukemia (CML) in blastic transformation (BT) referred to the Hammersmith Hospital from January 1995 to December 2000. BT was defined as the presence of at least 30% blasts in blood or marrow or e...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.7.2304
更新日期:2002-04-01 00:00:00