Abstract:
:Construction of 16S rDNA libraries was undertaken to examine the structure of free-living bacterial communities in the estuarine system Ria de Aveiro, Portugal. Samples were collected in April 2002, from two sites representing marine (station N1) and brackish (station I6) water zones. Clones were characterized by RFLP patterns and sequence analysis of representative clones revealed that both libraries were clearly dominated by alpha-proteobacteria, followed by gamma-proteobacteria and beta-proteobacteria. Clones affiliated with the delta-proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria were exclusive of station I6 and sequences related to the Firmicutes were only found in station N1. Sequences retrieved are included in only a few major bacterial divisions and in general, shared a high degree of homology with sequences deposited in nucleotide databases, and recovered from aquatic environments of diverse geographic regions. Differences between the two sites may reflect adaptation to different environmental conditions, especially salinity. The pattern of prokaryotic diversity is comparable to other coastal and estuarine environments previously studied.
journal_name
FEMS Microbiol Ecoljournal_title
FEMS microbiology ecologyauthors
Henriques IS,Almeida A,Cunha A,Correia Adoi
10.1016/j.femsec.2004.04.003subject
Has Abstractpub_date
2004-08-01 00:00:00pages
269-79issue
2eissn
0168-6496issn
1574-6941pii
FEM269journal_volume
49pub_type
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