In vivo bioluminescent imaging of Schwann cells in a poly(DL-lactide-epsilon-caprolactone) nerve guide.

Abstract:

:Nerve guides seeded with Schwann cells (SCs) promote axonal regeneration in peripheral nerve lesions. We examined the applicability of bioluminescent imaging (BLI) for monitoring the fate of SCs in nerve guides after implantation. Rat SCs were transfected with the firefly luciferase (Fluc) gene and subsequently seeded in nerve guides, which were implanted subcutaneously in rats. In vivo bioluminescence of transfected SCs (Fluc-SCs) was assessed with a BLI system. Scans were validated ex vivo using immunocytochemistry and electron microscopy. We found that BLI enables longitudinal in vivo monitoring of Fluc-SCs, given that proper access of luciferin to the cells is assured.

journal_name

Muscle Nerve

journal_title

Muscle & nerve

authors

Ma MS,Van Dam G,Meek M,Boddeke E,Copray S

doi

10.1002/mus.21372

subject

Has Abstract

pub_date

2009-11-01 00:00:00

pages

867-71

issue

5

eissn

0148-639X

issn

1097-4598

journal_volume

40

pub_type

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