Protein-induced membrane curvature investigated through molecular dynamics flexible fitting.

abstract：:Fusion is a crucial event in the infection of animal cells by enveloped viruses (e.g., HIV or influenza). Viral fusion is mediated by glycoproteins, spanning the viral envelope, which attach to a membrane surface and induce fusion of the viral envelope to the cellular membrane. Influenza fusion protein (hemagglutinin)...

journal_title：Biophysical journal

authors： Longo ML,Waring AJ,Hammer DA

更新日期：1997-09-01 00:00:00

abstract：:Molecular motors are thought to generate force and directional motion via nonequilibrium switching between energy surfaces. Because all enzymes can undergo such switching, we hypothesized that the ability to generate rotary motion and torque is not unique to highly adapted biological motor proteins but is instead a co...

journal_title：Biophysical journal

authors： Slochower DR,Gilson MK

更新日期：2018-05-08 00:00:00

abstract：:We developed a model of the rat ventricular myocyte at room temperature to predict the relative effects of different mechanisms on the cause of the slow increase in force in response to a step change in muscle length. We performed simulations in the presence of stretch-dependent increases in flux through the Na(+)-H(+...

journal_title：Biophysical journal

authors： Niederer SA,Smith NP

更新日期：2007-06-01 00:00:00

abstract：:The susceptibility of the Neurospora crassa (slime) cellular membrane to electroporation and electrodestruction in an alternating electric field was further investigated. The results were analyzed according to the dynamic rheological model of the cytoplasmic membrane. Based on an analytical description of membrane sus...

journal_title：Biophysical journal

authors： Pawlowski P,Szutowicz I,Rózycki S,Zieliński J,Fikus M

更新日期：1996-02-01 00:00:00

abstract：:We describe two enhancements of the planar bilayer recording method which enable low-noise recordings of single-channel currents activated by voltage steps in planar bilayers formed on apertures in partitions separating two open chambers. First, we have refined a simple and effective procedure for making small bilayer...

journal_title：Biophysical journal

authors： Wonderlin WF,Finkel A,French RJ

更新日期：1990-08-01 00:00:00

abstract：:The two-microelectrode, voltage-clamp technique was applied to rabbit cardiac Purkinje fibers to study the interaction of tetrodotoxin (TTX) with the slowly inactivating Na current. Binding of TTX to rested, inactivated, and activated channels was estimated by measuring the relative decrease of current at the beginnin...

journal_title：Biophysical journal

authors： Carmeliet E

更新日期：1987-01-01 00:00:00

abstract：:We have studied tubules formed from a polymerizable lipid in aqueous dispersion using freeze-fracture replication and transmission electron microscopy. The polymerizable diacetylenic lecithin 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine converts from liposomes to hollow cylinders, which we call tubules, o...

journal_title：Biophysical journal

authors： Yager P,Schoen PE,Davies C,Price R,Singh A

更新日期：1985-12-01 00:00:00

abstract：:Based on the existence of ATP-sensitive potassium channels in the plasma membrane of pancreatic beta cells, we develop a quantitative explanation of the electrical activity observed in pancreatic islets. The proposed mechanism involves the voltage-dependent inward calcium and outward potassium currents described by Ro...

journal_title：Biophysical journal

authors： Keizer J,Magnus G

更新日期：1989-08-01 00:00:00

abstract：:Bovine brain phosphatidylserine (BBPS) vesicles were prepared with traces of dioleoylglycerol (18:1, 18:1 DAG) or hexadecane (HD) to determine the influence of changes in headgroup or acyl chain packing on divalent cation-induced lipid mixing rates. A stopped-flow apparatus was used to combine vesicles with 3 mM Ca2+ ...

journal_title：Biophysical journal

authors： Walter A,Yeagle PL,Siegel DP

更新日期：1994-02-01 00:00:00

abstract：:Atomic force microscopy (AFM) is used to study mechanical properties of biological materials at submicron length scales. However, such samples are often structurally heterogeneous even at the local level, with different regions having distinct mechanical properties. Physical or chemical disruption can isolate individu...

journal_title：Biophysical journal

authors： Lee JJ,Rao S,Kaushik G,Azeloglu EU,Costa KD

更新日期：2018-06-05 00:00:00

abstract：:A 15-ns molecular dynamics (MD) simulation of the fully hydrated dimyristoylphosphatidylcholine-cholesterol (DMPC-Chol) bilayer in the liquid-crystalline state was carried out to investigate the effect of Chol on the hydrocarbon chain region of the bilayer. The last 8-ns fragment of the generated trajectory was used f...

journal_title：Biophysical journal

authors： Róg T,Pasenkiewicz-Gierula M

更新日期：2001-10-01 00:00:00

abstract：:Fluorescence resonance energy transfer (FRET) was used to reveal aspects of the mechanism of signal transduction by epidermal growth factor receptors (EGFR). The superpositions of epidermal growth factor (EGF), transforming growth factor-alpha (TGFalpha) and an antibody fragment (29.1) to the carbohydrate extremity of...

journal_title：Biophysical journal

authors： Martin-Fernandez M,Clarke DT,Tobin MJ,Jones SV,Jones GR

更新日期：2002-05-01 00:00:00

abstract：:Macromolecular transport between the nucleus and cytoplasm occurs through the nuclear pore complexes (NPCs). The NPC in the budding yeast Saccharomyces cerevisiae is a 60-MDa structure embedded in the nuclear envelope and composed of ~30 proteins, termed nucleoporins or nups. Here we present a large-scale analysis of ...

journal_title：Biophysical journal

authors： Damelin M,Silver PA

更新日期：2002-12-01 00:00:00

abstract：:Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to ...

journal_title：Biophysical journal

authors： Dammer U,Hegner M,Anselmetti D,Wagner P,Dreier M,Huber W,Güntherodt HJ

更新日期：1996-05-01 00:00:00

abstract：:Many complex cellular processes from mitosis to cell motility depend on the ability of the cytoskeleton to generate force. Force-generating systems that act on elastic cytoskeletal elements are prone to oscillating instabilities. In this work, we have measured spontaneous shape and movement oscillations in motile fish...

journal_title：Biophysical journal

authors： Barnhart EL,Allen GM,Jülicher F,Theriot JA

更新日期：2010-03-17 00:00:00

abstract：:Proteolysis of the multimeric blood coagulation protein von Willebrand Factor (VWF) by ADAMTS13 is crucial for prevention of microvascular thrombosis. ADAMTS13 cleaves VWF within the mechanosensitive A2 domain, which is believed to open under shear flow. In this study, we combine fluorescence correlation spectroscopy ...

journal_title：Biophysical journal

authors： Lippok S,Radtke M,Obser T,Kleemeier L,Schneppenheim R,Budde U,Netz RR,Rädler JO

更新日期：2016-02-02 00:00:00

abstract：:Rapid conduction of nerve impulses requires coating of axons by myelin sheaths, which are multilamellar, lipid-rich membranes produced by oligodendrocytes in the central nervous system. To act as an insulator, myelin has to form a stable and firm membrane structure. In this study, we have analyzed the biophysical prop...

journal_title：Biophysical journal

authors： Yurlova L,Kahya N,Aggarwal S,Kaiser HJ,Chiantia S,Bakhti M,Pewzner-Jung Y,Ben-David O,Futerman AH,Brügger B,Simons M

更新日期：2011-12-07 00:00:00

abstract：:The dynamical behavior of biomacromolecules is a fundamental property regulating a large number of biological processes. Protein dynamics have been widely shown to play a role in enzyme catalysis; however, the interplay between substrate dynamics and enzymatic activity is less understood. We report insights into the r...

journal_title：Biophysical journal

authors： Mercadante D,Melton LD,Jameson GB,Williams MA,De Simone A

更新日期：2013-04-16 00:00:00

abstract：:The use of propagation invariant Bessel beams has enabled high-resolution subcellular light sheet fluorescence microscopy. However, the energy within the concentric side lobe structure of Bessel beams increases significantly with propagation length, generating unwanted out-of-focus fluorescence that enforces practical...

journal_title：Biophysical journal

authors： Dean KM,Roudot P,Welf ES,Danuser G,Fiolka R

更新日期：2015-06-16 00:00:00

abstract：:Crystals of ion-free gramicidin A (P212121: a = 24.61, b = 32.28, c = 32.52) have been investigated using neutron diffraction. A difference analysis of crystals soaked in ethanol/H2O as opposed to ethanol-d6/D2O has led to single isomorphous replacement Fourier projections of the structure at 5-A resolution. The grami...

journal_title：Biophysical journal

authors： Koeppe RE 2nd,Schoenborn BP

更新日期：1984-03-01 00:00:00

abstract：:Atherosclerosis is a disease initiated by lipoprotein aggregation and deposition in artery walls. In this study, the de novo low-density lipoprotein aggregation process was examined. Nine major intermediates were identified in two stages of the aggregation process. In the aggregation stage, low-density lipoprotein mol...

journal_title：Biophysical journal

authors： Xu S,Lin B

更新日期：2001-10-01 00:00:00

abstract：:Formation of liquid-ordered domains in model membranes can be linked to raft formation in cellular membranes. The lipid stoichiometry has a governing influence on domain formation and consequently, biochemical hydrolysis of specific lipids has the potential to remodel domain features. Activation of phospholipase A(2) ...

journal_title：Biophysical journal

authors： Simonsen AC

更新日期：2008-05-15 00:00:00

abstract：:Efficient and rapid immune response upon challenge by an infectious agent is vital to host defense. The encounter of leukocytes (white blood cells of the immune system) with their targets is the first step in this response. Analysis of the kinetics of this process is essential not only to understanding dynamic behavio...

journal_title：Biophysical journal

authors： Charnick SB,Lauffenburger DA

更新日期：1990-05-01 00:00:00

abstract：:Computer simulations suggest that the translocation of arginine through the hydrocarbon core of a lipid membrane proceeds by the formation of a water-filled defect that keeps the arginine molecule hydrated even at the center of the bilayer. We show here that adding additional arginine molecules into one of these water...

journal_title：Biophysical journal

authors： MacCallum JL,Bennett WF,Tieleman DP

更新日期：2011-07-06 00:00:00

abstract：:We have used optical tweezers to study the elasticity of red cell membranes; force was applied to a bead attached to a permeabilized spherical ghost and the force-extension relation was obtained from the response of a second bead bound at a diametrically opposite position. Interruption of the skeletal network by disso...

journal_title：Biophysical journal

authors： Sleep J,Wilson D,Simmons R,Gratzer W

更新日期：1999-12-01 00:00:00

abstract：:The thermophilic Bacillus caldolyticus cold shock protein (Bc-Csp) differs from the mesophilic Bacillus subtilis cold shock protein B (Bs-CspB) in 11 of the 66 residues. Stability measurements of Schmid and co-workers have implicated contributions of electrostatic interactions to the thermostability. To further elucid...

journal_title：Biophysical journal

authors： Zhou HX,Dong F

更新日期：2003-04-01 00:00:00

abstract：:The conduction properties of ClC-0 and ClC-1 chloride channels are examined using electrostatic calculations and three-dimensional Brownian dynamics simulations. We create an open-state configuration of the prokaryotic ClC Cl(-) channel using its known crystallographic structure as a basis. Two residues that are occlu...

journal_title：Biophysical journal

authors： Corry B,O'Mara M,Chung SH

更新日期：2004-02-01 00:00:00

abstract：:We introduce an intrinsically multiplexed and easy to implement method to apply an external force to a biomolecule and thus probe its interaction with a second biomolecule or, more generally, its environment (for example, the cell membrane). We take advantage of the hydrodynamic interaction with a controlled fluid flo...

journal_title：Biophysical journal

authors： Türkcan S,Richly MU,Bouzigues CI,Allain JM,Alexandrou A

更新日期：2013-07-02 00:00:00

abstract：:Heart surface optical mapping of transmembrane potentials has been widely used in studies of normal and pathological heart rhythms and defibrillation. In these studies, three-dimensional spatio-temporal events can only be inferred from two-dimensional surface potential maps. We present a novel optical system that enab...

journal_title：Biophysical journal

authors： Hooks DA,LeGrice IJ,Harvey JD,Smaill BH

更新日期：2001-11-01 00:00:00

abstract：:Fluorescence correlation spectroscopy (FCS) has been used to measure the diffusion of fluorescently labeled beads in solutions of polymerized actin or buffer. The results, obtained at actin concentrations of 1 mg/ml, show that small beads (0.09 micron in diameter) diffuse nearly as rapidly in the actin gel as in buffe...

journal_title：Biophysical journal

authors： Qian H,Elson EL,Frieden C

更新日期：1992-10-01 00:00:00