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Comparative analysis of the membrane proteome of closely related metastatic and nonmetastatic tumor cells.

Abstract：

:The identification of proteins that are preferentially expressed on the membrane of metastatic tumor cells is of fundamental importance in cancer research. Here, we report the systematic comparison of the membrane proteome of two closely related murine teratocarcinoma cell lines (F9B9 and F9DR), of which only one (F9DR) is capable of forming liver metastases in vivo. The proteomic methodology used in this study featured the surface protein biotinylation on tumor cells followed by protein purification on streptavidin resin and relative quantification of corresponding tryptic peptides by mass spectrometric procedures. The study allowed the identification of 998 proteins and the determination of their relative abundance. Proteins previously known to be associated with metastatic spread were found to be either up-regulated (e.g., synaptojanin-2) or down-regulated (e.g., Ceacam1) in F9DR cells. A dramatic increase in abundance at the cell membrane was observed for a broad variety of proteins (e.g., high-mobility group protein B1), which were mainly thought to reside in intracellular compartments, a finding that was confirmed using confocal laser scanning microscopy and immunochemical analysis of cell cultures. Furthermore, we showed by microautoradiographic analysis that certain target proteins can readily be reached by intravenously administered radiolabeled antibodies. Finally, we showed that the most promising antigens for antibody-based pharmacodelivery approaches are strongly and selectively expressed on the surface of tumor cells in three different syngeneic mouse models of liver metastases. Taken together, our results indicate that the expression of intracellular proteins on the membrane of metastatic cells is a feature much more common than previously expected.

journal_name

Cancer Res

journal_title

Cancer research

authors

Roesli C,Borgia B,Schliemann C,Gunthert M,Wunderli-Allenspach H,Giavazzi R,Neri D

doi

10.1158/0008-5472.CAN-08-0999

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

5406-14

issue

13

eissn

0008-5472

issn

1538-7445

pii

0008-5472.CAN-08-0999

journal_volume

69

pub_type

杂志文章

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