Identification of proteomic changes during human liver development by 2D-DIGE and mass spectrometry.


BACKGROUND/AIMS:The aim of this study was to identify human liver proteins that are associated with different stages of liver development. METHODS:We collected liver samples from 14 fetuses between 14 and 41 weeks of development, one child and four adults. Proteins which exhibited consistent and significant variations during development by two-dimensional differential in gel electrophoresis (2D-DIGE) were subjected to peptide mass fingerprint analysis by MALDI-TOF mass spectrometry. Real-time PCR analysis confirmed, at the transcriptional level, the data obtained by the proteomic approach. RESULTS:Among a total of 80 protein spots showing differential expression, we identified 42 different proteins or polypeptide chains, of which 26 were upregulated and 16 downregulated in developing in comparison to adult liver. These proteins could be classified in specific groups according to their function. By comparing their temporal expression profiles, we identified protein groups that were associated with different developmental stages of human fetal liver and suggest that the changes in protein expression observed during the 20- to 36-week time window play a pivotal role in liver development. CONCLUSIONS:The identification of these proteins may represent good markers of human liver and stem cells differentiation.


J Hepatol


Journal of hepatology


Brizard JP,Ramos J,Robert A,Lafitte D,Bigi N,Sarda P,Laoudj-Chenivesse D,Navarro F,Blanc P,Assenat E,Maurel P,Pascussi JM,Vilarem MJ




Has Abstract


2009-07-01 00:00:00














  • Non-radioactive hybridization with hepatitis C virus-specific probes created during polymerase chain reaction: a fast and simple procedure to verify hepatitis C virus infection.

    abstract::All routinely available assays to detect hepatitis C virus (HCV) infection are based on the specific reaction of serum antibodies to corresponding viral antigens. The only method to detect viral nucleic acids in serum or liver is the enzymatic amplification of reversely transcribed HCV-RNA by polymerase chain reaction...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Geiger CP,Caselmann WH

    更新日期:1992-07-01 00:00:00

  • Induction of chronic cholangitis in the rat by trinitrobenzenesulfonic acid.

    abstract::We investigated the effects of the hapten trinitrobenzenesulfonic acid instilled into the rat biliary tree. The study included three groups of animals that received a single intracholedochal injection of either saline, 10% ethanol or 10 mg trinitrobenzenesulfonic acid dissolved in 10% ethanol. A fourth group of rats w...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Mourelle M,Salas A,Vilaseca J,Guarner F,Malagelada JR

    更新日期:1995-02-01 00:00:00

  • Incidence of hepatocellular carcinoma in HIV/HBV-coinfected patients on tenofovir therapy: Relevance for screening strategies.

    abstract:BACKGROUND & AIMS:Robust data on hepatocellular carcinoma (HCC) incidence among HIV/hepatitis B virus (HBV)-coinfected individuals on antiretroviral therapy (ART) are needed to inform HCC screening strategies. We aimed to evaluate the incidence and risk factors of HCC among HIV/HBV-coinfected individuals on tenofovir d...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Wandeler G,Mauron E,Atkinson A,Dufour JF,Kraus D,Reiss P,Peters L,Dabis F,Fehr J,Bernasconi E,van der Valk M,Smit C,Gjærde LK,Rockstroh J,Neau D,Bonnet F,Rauch A,Swiss HIV Cohort Study, Athena Observational Cohort Study

    更新日期:2019-08-01 00:00:00

  • Expression of HLA antigens and T cell infiltrates in chronic viral hepatitis. A comparison of biopsy and fine-needle aspiration findings.

    abstract::To evaluate the expression of hepatocyte HLA Class I and II antigens and the infiltration of CD8-positive lymphocytes in patients with chronic hepatitis, cells obtained by the technique of fine-needle aspiration were compared with those obtained by percutaneous liver biopsy. In general, correlation between cytological...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Krawitt EL,Zannier A,Chossegros P,Gerard F,Chevallier M,Mutin M,Trepo C,Touraine JL

    更新日期:1991-03-01 00:00:00

  • Pediatric gallstone disease in familial hypobetalipoproteinemia.

    abstract::Familial hypobetalipoproteinemia (FHBL) is an monogenic co-dominant disorder characterized by reduced plasma levels of cholesterol, low density lipoproteins (LDL) and apolipoprotein B (apoB) often associated with non-alcoholic fatty liver disease (NAFLD). It has been suggested that FHBL might predispose to gallstone d...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Lancellotti S,Zaffanello M,Di Leo E,Costa L,Lonardo A,Tarugi P

    更新日期:2005-07-01 00:00:00

  • Resveratrol improves intrahepatic endothelial dysfunction and reduces hepatic fibrosis and portal pressure in cirrhotic rats.

    abstract:BACKGROUND & AIMS:Resveratrol, a polyphenol found in a variety of fruits, exerts a wide range of beneficial effects on the endothelium, regulates multiple vasoactive substances and decreases oxidative stress, factors involved in the pathophysiology of portal hypertension. Our study aimed at evaluating the effects of re...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Di Pascoli M,Diví M,Rodríguez-Vilarrupla A,Rosado E,Gracia-Sancho J,Vilaseca M,Bosch J,García-Pagán JC

    更新日期:2013-05-01 00:00:00

  • SIRT1 regulates oncogenesis via a mutant p53-dependent pathway in hepatocellular carcinoma.

    abstract:BACKGROUND & AIMS:SIRT1 is a class III histone deacetylase that plays diverse roles in various cancers. However, the clinical significance of SIRT1 in hepatocellular carcinoma (HCC) remains unknown. METHODS:We analysed p53 mutations and the activation of SIRT1 in 252 hepatitis B virus-positive HCC cases. None of the p...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Zhang ZY,Hong D,Nam SH,Kim JM,Paik YH,Joh JW,Kwon CH,Park JB,Choi GS,Jang KY,Park CK,Kim SJ

    更新日期:2015-01-01 00:00:00

  • Adaptive changes of hepatic bile salt transport in a model of reversible interruption of the enterohepatic circulation in the rat.

    abstract::The reversibility and time course of the adaptive changes in hepatic bile salt transport related to modifications of the bile salt enterohepatic circulation and bile salt pool size have not been previously studied. For this reason a model of reversible interruption of entero-hepatic circulation was characterized in un...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Accatino L,Hono J,Koenig C,Pizarro M,Rodriguez L

    更新日期:1993-08-01 00:00:00

  • The changing scenario of hepatocellular carcinoma over the last two decades in Italy.

    abstract:BACKGROUND & AIMS:This study investigates whether the aetiologic changes in liver disease and the improved management of hepatocellular carcinoma (HCC) have modified the clinical scenario of this tumour over the last 20 years in Italy. METHODS:Retrospective study based on the analysis of the ITA.LI.CA (Italian Liver C...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Santi V,Buccione D,Di Micoli A,Fatti G,Frigerio M,Farinati F,Del Poggio P,Rapaccini G,Di Nolfo MA,Benvegnù L,Zoli M,Borzio F,Giannini EG,Caturelli E,Chiaramonte M,Bernardi M,Trevisani F

    更新日期:2012-02-01 00:00:00

  • Protective effects of FUT-175 on acute massive hepatic necrosis induced in mice following endotoxin injection and immunization with liver proteins.

    abstract::Experimental autoimmune hepatitis was induced in C57BL/6 mice by immunization with syngeneic liver protein and adjuvant. Hepatitis was characterized by marked cellular infiltrates, but hepatic necrosis was mild to moderate. A small dose of endotoxin (25 micrograms/mouse) produced lethal hepatitis with elevation of ser...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Ogawa M,Mori Y,Ueda S,Mori T,Makino Y,Hori J,Ohto M,Wakashin M

    更新日期:1993-11-01 00:00:00

  • Subclinical impairment of brain function in chronic hepatitis C infection.

    abstract:BACKGROUND/AIMS:Central nervous system abnormalities such as fatigue and depression occur more frequently in chronic hepatitis C virus (HCV) infection than in many other causes of chronic liver disease. The finding that fatigue is unrelated to activity of hepatitis or mode of infection could indicate an independent eff...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Kramer L,Bauer E,Funk G,Hofer H,Jessner W,Steindl-Munda P,Wrba F,Madl C,Gangl A,Ferenci P

    更新日期:2002-09-01 00:00:00

  • Involvement of natural killer cells in PolyI:C-induced liver injury.

    abstract:BACKGROUND/AIMS:The roles of T cells, natural killer T cells (NKT) and macrophages in autoimmune hepatitis have been well documented. However, the roles of natural killer (NK) cells in liver injury remain obscure. Here we examined the effect of Polyinosinic: polycytidylic acid (PolyI:C)-activated NK cells on liver inju...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Dong Z,Wei H,Sun R,Hu Z,Gao B,Tian Z

    更新日期:2004-12-01 00:00:00

  • Release of lysosomal enzymes is not correlated with superoxide and prostaglandin production by stimulated rat Kupffer cells in primary culture.

    abstract::A substantial production of prostaglandin E2 (PGE2) was induced in primary cultures of rat Kupffer cells by zymosan, calcium ionophore A23187, phorbol ester and arachidonic acid, whereas contact with latex particles, glucan or immunocomplexes led to a minor PGE2 release only. Superoxide generation, on the other hand, ...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Dieter P,Schulze-Specking A,Decker K

    更新日期:1988-04-01 00:00:00

  • Abundant numbers of regulatory T cells localize to the liver of chronic hepatitis C infected patients and limit the extent of fibrosis.

    abstract:BACKGROUND & AIMS:Weak hepatitis C virus (HCV) specific immunity in peripheral blood has been shown to be partially controlled by regulatory T cells (Treg). However, little is known about Treg present in livers of HCV-infected patients, their association with clinical parameters, and immunopathology resulting in diseas...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Claassen MA,de Knegt RJ,Tilanus HW,Janssen HL,Boonstra A

    更新日期:2010-03-01 00:00:00

  • Cost-effectiveness of semi-annual surveillance for hepatocellular carcinoma in cirrhotic patients of the Italian Liver Cancer population.

    abstract:BACKGROUND & AIMS:It was recently shown that semi-annual surveillance for hepatocellular carcinoma (HCC) in cirrhotic patients provides a prognostic advantage over the annual program; however, its cost-effectiveness (CE) in the general cirrhotic population still needs to be defined. METHODS:A Markov model was built to...

    journal_title:Journal of hepatology

    pub_type: 杂志文章,多中心研究


    authors: Cucchetti A,Trevisani F,Cescon M,Ercolani G,Farinati F,Poggio PD,Rapaccini G,Nolfo MAD,Benvegnù L,Zoli M,Borzio F,Giannini EG,Caturelli E,Chiaramonte M,Pinna AD,Italian Liver Cancer (ITA.LI.CA) Group.

    更新日期:2012-05-01 00:00:00

  • New virological tools for screening, diagnosis and monitoring of hepatitis B and C in resource-limited settings.

    abstract::Worldwide, the increasingly dominant model of laboratory testing is the centralised laboratory, in which automation of analytical processes increases, enabling the analysis of large numbers of samples at a relatively low cost. However, this trend does not fulfil the requirements for care of patients with chronic hepat...

    journal_title:Journal of hepatology

    pub_type: 杂志文章,评审


    authors: Chevaliez S,Pawlotsky JM

    更新日期:2018-10-01 00:00:00

  • Chronic ethanol feeding increases apoptosis and cell proliferation in rat liver.

    abstract::The present study was conducted to evaluate if the increased rate of apoptosis previously reported in the liver of ethanol-treated rats was accompanied by increased cell renewal. A quantitative analysis of apoptosis was performed in rats fed an ethanol-containing liquid diet for 5 weeks. S-phase cells were demonstrate...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Baroni GS,Marucci L,Benedetti A,Mancini R,Jezequel AM,Orlandi F

    更新日期:1994-04-01 00:00:00

  • Different expression of HLA-DR and ICAM-1 in livers from patients with biliary atresia and Byler's disease.

    abstract:BACKGROUND/AIMS:Extrahepatic biliary atresia (EHBA) and chronic progressive intra-hepatic cholestasis (Byler's disease) are two distinct disorders of unknown etiology in the neonate, characterized by profound cholestasis. The aim of the present study was to investigate the inflammatory reaction in liver tissue from pat...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Broomé U,Nemeth A,Hultcrantz R,Scheynius A

    更新日期:1997-04-01 00:00:00

  • Heterogeneity of hepatitis B virus C-gene sequences: implications for amplification and sequencing.

    abstract::Occasionally direct sequencing of amplified hepatitis B virus DNA leads to weak signals on autoradiograms. Using amplified C-gene sequences we investigated whether this is due to sequence heterogeneity of virus populations and use of inappropriate primers for direct sequencing. High C-gene sequence heterogeneity (poin...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Miska S,Günther S,Vassilev M,Meisel H,Pape G,Will H

    更新日期:1993-04-01 00:00:00

  • Replicating and virion secreting hepatitis B mutant virus unable to produce preS2 protein.

    abstract::Mutant HBV genomes unable to produce preS2 protein were identified in the serum of a highly viremic chronic carrier. By direct sequencing of the amplified preS region and by sequencing of 50 cloned amplified preS DNA fragments all molecules were found to have deleted the preS2 translation initiation codon and three am...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Fernholz D,Stemler M,Brunetto M,Bonino F,Will H

    更新日期:1991-01-01 00:00:00

  • A patient with novel ABCB11 gene mutations with phenotypic transition between BRIC2 and PFIC2.

    abstract::We describe a PFIC2 patient with a good response to ursodeoxycholic acid for 9 years. We found two novel ABCB11 gene mutations in the patient, i.e. I498T and 2098delA. The correlation of the patient's genotypes with the clinical course supports the existence of a phenotypic continuum between BRIC2 and PFIC2. ...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Lam CW,Cheung KM,Tsui MS,Yan MS,Lee CY,Tong SF

    更新日期:2006-01-01 00:00:00

  • Nicardipine increases hepatic blood flow and the hepatic clearance of indocyanine green in patients with cirrhosis.

    abstract::The present study investigated the hemodynamic effects of nicardipine, a new calcium channel blocker, and placebo in 14 patients with cirrhosis. Sixty minutes after nicardipine administration (20 mg orally; n = 8), there was a significant increase in hepatic blood flow (25 +/- 21%; p < 0.05) and azygos blood flow (33 ...

    journal_title:Journal of hepatology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: García-Pagán JC,Feu F,Luca A,Fernández M,Pizcueta P,Bosch J,Rodés J

    更新日期:1994-06-01 00:00:00

  • Recombinant human manganese superoxide dismutase reduces liver fibrosis and portal pressure in CCl4-cirrhotic rats.

    abstract:BACKGROUND & AIMS:High oxidative stress plays a major role in increasing hepatic vascular resistance in cirrhosis, by facilitating liver fibrosis and by increasing hepatic vascular tone. This study is aimed at investigating whether the use of the novel isoform of recombinant human manganese superoxide dismutase (rMnSOD...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Guillaume M,Rodriguez-Vilarrupla A,Gracia-Sancho J,Rosado E,Mancini A,Bosch J,Garcia-Pagán JC

    更新日期:2013-02-01 00:00:00

  • Pattern of HCV antibodies with special reference to NS5A reactivity in HCV-infected patients: relation to viral genotype, cryoglobulinemia and response to interferon.

    abstract:BACKGROUND/AIMS:We aimed to compare the anti-hepatitis C virus reactivity in confirmatory assays (RIBA 3.0 Ortho Diagnostic and INNO-LIA HCV Ab III Innogenetics) among patients infected with different hepatitis C virus genotypes, with or without cryoglobulinemia, and in patients treated with interferon. METHODS:One hu...

    journal_title:Journal of hepatology

    pub_type: 临床试验,杂志文章


    authors: Frangeul L,Cresta P,Perrin M,Duverlie G,Khorsi H,Musset L,Opolon P,Huraux JM,Lunel F

    更新日期:1998-04-01 00:00:00

  • Regulatory T cells inhibit Th1 cell-mediated bile duct injury in murine biliary atresia.

    abstract:BACKGROUND & AIMS:Biliary atresia (BA) is a pediatric inflammatory disease of the biliary system which leads to cirrhosis and the need for liver transplantation. One theory regarding etiology is that bile duct injury is due to virus-induced autoreactive T cell-mediated inflammation. Regulatory T cell (Treg) abnormaliti...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Tucker RM,Feldman AG,Fenner EK,Mack CL

    更新日期:2013-10-01 00:00:00

  • Purinergic P2Y2 receptors promote hepatocyte resistance to hypoxia.

    abstract:BACKGROUND/AIMS:ATP stimulation of purinergic P2 receptors (P2YR and P2XR) regulates several hepatic functions. Here we report the involvement of ATP-mediated signals in enhancing hepatocyte tolerance to lethal stress. METHODS:The protection given by purinergic agonists was investigated in rat hepatocytes exposed to h...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Carini R,Alchera E,De Cesaris MG,Splendore R,Piranda D,Baldanzi G,Albano E

    更新日期:2006-08-01 00:00:00

  • Evaluation and comparison of different hepatitis C virus genotyping and serotyping assays.

    abstract:BACKGROUND/AIMS:Evidence that the geno/subtype of hepatitis C virus (HCV) is predictive of the response to interferon-alpha therapy suggests that typing methods are clinically useful. In the present study, HCV isolates obtained from 74 patients with chronic hepatitis C were used to evaluate three genotyping and two ser...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Lee JH,Roth WK,Zeuzem S

    更新日期:1997-05-01 00:00:00

  • Non-invasive diagnosis of hepatocellular carcinoma ≤ 2 cm in cirrhosis. Diagnostic accuracy assessing fat, capsule and signal intensity at dynamic MRI.

    abstract:BACKGROUND & AIMS:To prospectively assess the diagnostic accuracy of the incorporation of additional magnetic resonance imaging (MRI) parameters in those based on contrast enhancement pattern for the diagnosis of solitary nodules between 5 and 20mm, detected during surveillance in patients with cirrhosis. METHODS:Betw...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Rimola J,Forner A,Tremosini S,Reig M,Vilana R,Bianchi L,Rodríguez-Lope C,Solé M,Ayuso C,Bruix J

    更新日期:2012-06-01 00:00:00

  • Antiviral activity of TMC435 monotherapy in patients infected with HCV genotypes 2-6: TMC435-C202, a phase IIa, open-label study.

    abstract:BACKGROUND & AIMS:TMC435 is an investigational, once-daily, oral NS3/4A protease inhibitor currently in phase III development for the treatment of hepatitis C virus (HCV) infection. Phase I and II studies in patients infected with HCV genotype 1 have demonstrated that TMC435 is generally well tolerated, has a pharmacok...

    journal_title:Journal of hepatology

    pub_type: 杂志文章,多中心研究


    authors: Moreno C,Berg T,Tanwandee T,Thongsawat S,Van Vlierberghe H,Zeuzem S,Lenz O,Peeters M,Sekar V,De Smedt G

    更新日期:2012-06-01 00:00:00

  • Role of the liver-enriched transcription factors C/EBP alpha and DBP in the expression of human CYP3A4 and CYP3A7.

    abstract::In the human fetal liver, CYP3A7 is expressed as early as the 13th week of gestation. This continues to the perinatal period when it is sharply repressed prior to birth. Concomitantly, the expression of CYP3A4, not detectable in the fetus, sharply increases in the perinatal period to remain elevated throughout adultho...

    journal_title:Journal of hepatology

    pub_type: 杂志文章


    authors: Ourlin JC,Jounaïdi Y,Maurel P,Vilarem MJ

    更新日期:1997-01-01 00:00:00