The role of specific cytochromes P450 in the formation of 7,12-dimethylbenz(a)anthracene-protein adducts in rat liver microsomes in vitro.

abstract：UNLABELLED:Non-steroidal anti-inflammatory drugs (NSAIDs) induce the shedding of L-selectin in human neutrophils through a mechanism still not well understood. In this work we studied both the functional effect of NSAIDs on the neutrophils/endothelial cells dynamic interaction, and the potential involvement of reactive...

journal_title：Biochemical pharmacology

authors： Domínguez-Luis M,Herrera-García A,Arce-Franco M,Armas-González E,Rodríguez-Pardo M,Lorenzo-Díaz F,Feria M,Cadenas S,Sánchez-Madrid F,Díaz-González F

更新日期：2013-01-15 00:00:00

abstract：:Recent studies in our laboratory have revealed that Mn2+ is capable of promoting cell spreading and neurite outgrowth in PC12 cells, a process which is dependent on Mn2+ stimulation of the interaction between extracellular matrix (ECM) components and their corresponding integrin receptors. Since the major ECM proteins...

journal_title：Biochemical pharmacology

authors： Lin WH,Marcucci K,Roth JA

更新日期：1994-04-29 00:00:00

abstract：:The salt-induced condensation of chromatin has been studied with flow-linear dichroism technique using an intercalative dye (methylene blue) to selectively monitor the linker orientation. At low ionic strength both linkers and chromatosomes (with their flat faces) are oriented preferentially parallel to the chromatin ...

journal_title：Biochemical pharmacology

authors： Kubista M,Nielsen PE,Nordén B

更新日期：1988-05-01 00:00:00

abstract：:Adult hepatocytes from rat and man were maintained for 2 weeks between two gel layers in a sandwich configuration to study the influence of this culture technique on the preservation of basal activities of xenobiotic-metabolizing phase I and phase II enzymes. The response of these enzyme activities to an enzyme induce...

journal_title：Biochemical pharmacology

authors： Kern A,Bader A,Pichlmayr R,Sewing KF

更新日期：1997-10-01 00:00:00

abstract：:The cysteine (Cys) precursor 2(RS)-n-propylthiazolidine-4(R)-carboxylic acid (PTCA) has been shown to protect against acetaminophen (APAP)-induced hepatic GSH, GSSG, and Cys depletion and hepatic necrosis. The aim of this study was to determine the effects of PTCA on the concentrations of sulfhydryl compounds in extra...

journal_title：Biochemical pharmacology

authors： Srinivasan C,Williams WM,Nagasawa HT,Chen TS

更新日期：2001-04-01 00:00:00

abstract：OBJECTIVE:The evolution of the "cholinergic anti-inflammatory pathway" and the fact that the α 7 subunit of the nicotinic acetylcholine receptor (α7nAChR) is present in the spleen, joint and on the surface of lymphocytes, opened up the prospective in this study of targeting the α7nAChR by the anticholinesterase and cho...

journal_title：Biochemical pharmacology

authors： Gowayed MA,Rothe K,Rossol M,Attia AS,Wagner U,Baerwald C,El-Abhar HS,Refaat R

更新日期：2019-12-01 00:00:00

abstract：:Studies were performed to investigate the irreversible binding and oxidative metabolism of propranolol in human liver microsomes and the relationship of binding and metabolism to the polymorphic oxidation of debrisoquine. Incubation of microsomes with 14C-labelled propranolol in the presence of a NADPH-generating syst...

journal_title：Biochemical pharmacology

authors： Shaw L,Lennard MS,Tucker GT,Bax ND,Woods HF

更新日期：1987-07-15 00:00:00

abstract：:Studies have shown that CYP2C9.1 mediated metabolism of flurbiprofen or naproxen is activated by co-incubation with dapsone. However, dapsone activation has not been examined in the known variant forms of CYP2C9. Six concentrations of flurbiprofen (2-300microM) or naproxen (10-1800 microM) were co-incubated with six c...

journal_title：Biochemical pharmacology

authors： Hummel MA,Dickmann LJ,Rettie AE,Haining RL,Tracy TS

更新日期：2004-05-15 00:00:00

abstract：:Only limited data are available on the inhibition of the sugar transporter GLUT5 by flavonoids or other classes of bioactives. Intestinal GLUT7 is poorly characterised and no information exists concerning its inhibition. We aimed to study the expression of GLUT7 in Caco-2/TC7 intestinal cells, and evaluate inhibition ...

journal_title：Biochemical pharmacology

authors： Gauer JS,Tumova S,Lippiat JD,Kerimi A,Williamson G

更新日期：2018-06-01 00:00:00

abstract：:The effects of taurine (2-aminoethanesulphonic acid) and its analogues, 2-aminoethylarsonic acid, 2-hydroxyethanesulphonic (isethionic) acid, 3-aminopropanesulphonic acid, 2-aminoethylphosphonic acid, and N,N-dimethyltaurine, were studied on the transport of Ca2+ by mitochondria isolated from rat liver. Taurine enhanc...

journal_title：Biochemical pharmacology

authors： Palmi M,Youmbi GT,Fusi F,Sgaragli GP,Dixon HB,Frosini M,Tipton KF

更新日期：1999-10-01 00:00:00

abstract：:Hypertension is considered as one of the cancer progressive factors, and often found comorbidity in cancer patients. Renin-angiotensin system (RAS) plays an important role in the regulation of blood pressure, and angiotensin II (Ang II) is well known pressor peptide associated with RAS. Ang II has been reported to acc...

journal_title：Biochemical pharmacology

authors： Ishikane S,Hosoda H,Nojiri T,Tokudome T,Mizutani T,Miura K,Akitake Y,Kimura T,Imamichi Y,Kawabe S,Toyohira Y,Yanagihara N,Takahashi-Yanaga F,Miyazato M,Miyamoto K,Kangawa K

更新日期：2018-08-01 00:00:00

abstract：:7-Ketocholesterol (7-KC) is found at an elevated level in patients with cancer and chronic liver disease. The up-regulation of an efflux pump, P-glycoprotein (P-gp) leads to drug resistance. To elucidate the effect of 7-KC on P-gp, P-gp function and expression were investigated in hepatoma cell lines Huh-7 and HepG2 a...

journal_title：Biochemical pharmacology

authors： Wang SF,Chou YC,Mazumder N,Kao FJ,Nagy LD,Guengerich FP,Huang C,Lee HC,Lai PS,Ueng YF

更新日期：2013-08-15 00:00:00

abstract：:Previous studies from this lab and others have demonstrated that nitric oxide (NO) in a concentration dependent manner, modulated neutrophil and leukemic cell survival. Subsequent studies delineated importance of iNOS in neutrophil differentiation and leukemic cell death. On the contrary, role of nNOS in survival of t...

journal_title：Biochemical pharmacology

authors： Sadaf S,Awasthi D,Singh AK,Nagarkoti S,Kumar S,Barthwal MK,Dikshit M

更新日期：2020-06-01 00:00:00

abstract：:Ultrastructural morphology (transmission electron microscopy) and localisation of cisplatin-induced platinum (Pt)-DNA adducts (immunoelectron microscopy) were analysed in the human small cell lung cancer cell line GLC(4) and its 40-fold in vitro acquired cisplatin-resistant subline GLC(4)-CDDP, which is characterised ...

journal_title：Biochemical pharmacology

authors： Meijera C,van Luyn MJ,Nienhuis EF,Blom N,Mulder NH,de Vries EG

更新日期：2001-03-01 00:00:00

abstract：:Interleukin-2 (IL-2) has been shown to decrease cytochrome P450 (CYP) mRNAs and proteins in cultured rat hepatocytes, and IL-2 administration decreases CYPs in rats. Although high doses of IL-2 are administered to cancer patients, the effect on human CYPs has not yet been determined. Patients with hepatic metastases f...

journal_title：Biochemical pharmacology

authors： Elkahwaji J,Robin MA,Berson A,Tinel M,Lettéron P,Labbe G,Beaune P,Elias D,Rougier P,Escudier B,Duvillard P,Pessayre D

更新日期：1999-04-15 00:00:00

abstract：:Benzbromarone, a potent uricosuric agent, inhibited allantoin production in isolated hepatocytes at concentrations half to ten times greater than therapeutic plasma levels of the drug. In addition, the drug at these concentrations also markedly inhibited xanthine oxidase (EC 1.2.1.37), an enzyme involved in the regula...

journal_title：Biochemical pharmacology

authors： Rodilla F,Sanchez-Beltran MJ,Izquierdo R,Gomez-Ruiz MD,Cabo J

更新日期：1988-10-01 00:00:00

abstract：:Alone and in synergistic combination with retinoids, dietary glucarate inhibits both the chemical induction and growth of rat mammary tumors. To investigate the pharmacokinetics of glucarate, [14C]glucarate was synthesized, converted to the calcium salt, and administered to rats bearing primary mammary tumors. When gi...

journal_title：Biochemical pharmacology

authors： Webb TE,Pham-Nguyen MH,Darby M,Hamme AT 2nd

更新日期：1994-04-29 00:00:00

abstract：:The murine Cyp2a-4 and Cyp2a-5 genes encode P450 isoforms catalysing testosterone 15 alpha-hydroxylase and coumarin 7-hydroxylase (COH) activities, respectively. Two days after the administration of a hepatotoxic dose of cerium chloride (2 mg/kg i.v.), COH activity was increased 3.2-fold in the liver of DBA/2 mice. Th...

journal_title：Biochemical pharmacology

authors： Salonpää P,Iscan M,Pasanen M,Arvela P,Pelkonen O,Raunio H

更新日期：1992-10-06 00:00:00

abstract：:A comparison of the effects of various phosphodiesterase (PDE) inhibitors upon cellular cAMP levels was undertaken in human neuroblastoma SH-SY5Y cells. When inhibitors such as rolipram and Ro 20 1724 (selective for the low Km cAMP-specific PDE) were used, cAMP levels were seen to rise dramatically under basal (< or =...

journal_title：Biochemical pharmacology

authors： Morgan AJ,Murray KJ,Challiss RA

更新日期：1993-06-22 00:00:00

abstract：:The relative ease of generation of reactive oxygen species from a series of reductively activated aglycone and sugar modified anthracyclines was compared by the extents of single strand scission in supercoiled PM2-covalently closed circular (CCC)-DNA. The electrochemical properties of these agents were used as a quant...

journal_title：Biochemical pharmacology

authors： Lown JW,Chen HH,Plambeck JA,Acton EM

更新日期：1982-02-15 00:00:00

abstract：:Nitric oxide sensitive guanylyl cyclase (NOsGC) is a heterodimeric enzyme consisting of an α and a β subunit. Two heterodimeric enzymes are known to be important for NO-signalling in humans: α(1)/β(1) and α(2)/β(1). No difference had so far been detected with respect to their pharmacological properties, but as we show...

journal_title：Biochemical pharmacology

authors： Haase N,Haase T,Kraehling JR,Behrends S

更新日期：2010-12-01 00:00:00

abstract：:The fallen concentration of one of the two isomers of soman (1,2,2-trimethylpropyl methylphosphonofluoridate), i.e., C(+)P(-)-soman, was investigated in plasma and in homogenates of brain, lung, liver, kidney, diaphragm, skeletal muscle and mucosa of small intestines from rat, guinea pig and marmoset, and in human pla...

journal_title：Biochemical pharmacology

authors： de Jong LP,van Dijk C,Berhitoe D,Benschop HP

更新日期：1993-10-19 00:00:00

abstract：:The recombinant plasmid pBHIV1 carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1), linked to the chloramphenicol acetyl transferase (CAT) gene, was introduced into human and rat fibroblasts. Stable transfectants resistant to geneticin expressed CAT activity from the HIV-1 LTR. It was...

journal_title：Biochemical pharmacology

authors： Zoumpourlis V,Kerr DJ,Spandidos DA

更新日期：1992-02-04 00:00:00

abstract：:Blocking spermidine and spermine synthesis in Plasmodium falciparum-infected erythrocytes with irreversible inhibitors of S-adenosylmethionine decarboxylase (AdoMet DC; EC 4.1.1.50), prevented the growth of the parasite in vitro. The most potent of these compounds, MDL 73811, inhibited growth of chloroquine-sensitive ...

journal_title：Biochemical pharmacology

authors： Wright PS,Byers TL,Cross-Doersen DE,McCann PP,Bitonti AJ

更新日期：1991-06-01 00:00:00

abstract：:Irradiation of a cytosolic fraction from vascular smooth muscle in the presence of [3H]felodipine resulted in the labelling of a protein with an apparent molecular weight of 62 kDa. The labelling was seen on UV-irradiation at 360 nm, but not at 254, 278 or at wavelengths above 410 nm. The photolabelling was enhanced i...

journal_title：Biochemical pharmacology

authors： Nilsson A,Westerlund C,Boström S

更新日期：1989-12-01 00:00:00

abstract：:The dithiol-reducing thioredoxin/thioredoxin reductase system normally maintains the reduced state of key enzymes responsible for the cell's anti-oxidant defences. We therefore addressed the question of whether AW 464--a novel thioredoxin inhibitor--as well as broad spectrum dithiol ligands diamide and phenylarsine ox...

journal_title：Biochemical pharmacology

authors： Pallis M,Bradshaw TD,Westwell AD,Grundy M,Stevens MF,Russell N

更新日期：2003-11-01 00:00:00

abstract：:Aldehyde oxidase (AOX) is a cytosolic enzyme responsible for the metabolism of some drugs and drug candidates. AOX catalyzes the oxidative hydroxylation of substrates including several aliphatic and aromatic aldehydes, and nitrogen-containing heterocyclic compounds. AOX is also reported to catalyze the reductive metab...

journal_title：Biochemical pharmacology

authors： Paragas EM,Humphreys SC,Min J,Joswig-Jones CA,Jones JP

更新日期：2017-12-01 00:00:00

abstract：:Epidemiological and experimental animal data indicate that exposure to both metals and metalloid species exacerbates the risk of human diseases, particularly cancers. Vascular endothelial growth factor (VEGF), which performs a primary function in both tumor progression and angiogenesis, is up-regulated due to exposure...

journal_title：Biochemical pharmacology

authors： Lee M,Hwang JT,Yun H,Kim EJ,Kim MJ,Kim SS,Ha J

更新日期：2006-06-28 00:00:00

abstract：:We studied whether gemfibrozil and clofibric acid alter isoprenoid lipid synthesis in rat hepatocytes. After incubation of the cells with the agent for 74 hr, [(14)C]acetate or [(3)H]mevalonate was added, and the cells were further incubated for 4 hr. Gemfibrozil and clofibric acid increased ubiquinone synthesis from ...

journal_title：Biochemical pharmacology

authors： Hashimoto F,Taira S,Hayashi H

更新日期：2000-05-15 00:00:00

abstract：:Chronic exposure to the divalent heavy metals, such as iron, lead, manganese (Mn), and chromium, has been linked to the development of severe, often irreversible neurological disorders and increased vulnerability to developing Parkinson's disease. Although the mechanisms by which these metals elicit or facilitate neur...

journal_title：Biochemical pharmacology

authors： Roth JA,Garrick MD

更新日期：2003-07-01 00:00:00