Survival of Staphylococcus and other bacteria in skin prick test antigens solutions.

Abstract:

BACKGROUND:Skin prick tests are commonly used for human allergy testing and determination. Because solutions are kept at 4 degrees C and patient's skin is cleaned, the same solutions are used on many patients with the same applicators without sterilization. OBJECTIVES:Our objectives were to determine whether a model bacteria could survive in antigen solutions and whether bacteria from patient's skin could be found in clinical antigen solutions. METHODS:Unused antigen solutions were spiked using Staphylococcus epidermidis. Concentration was followed from time = 0 to time = 21 days. Clinical samples consisted of antigen solutions used on a daily basis, from different hospital centers (n = 146). These were tested for total bacterial counts and detection of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci. Isolates were identified by 16S rDNA sequencing. RESULTS:The concentration of S epidermidis remained stable over the 21 days. Twenty-three percent of the clinical antigen solutions contained bacteria. As expected, species like S aureus, S hominis, S epidermidis, and Micrococcus luteus were highly represented (62.5%) within a total of 20 different species. CONCLUSION:Skin bacteria can survive in antigen solutions used in skin prick tests. Moreover, even if no MRSA were observed in field samples, nosocomial infections could be a concern if pathogens were present on the skin of some tested patients.

journal_name

Am J Infect Control

authors

Veillette M,Cormier Y,Duchaine C

doi

10.1016/j.ajic.2008.11.007

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

606-8

issue

7

eissn

0196-6553

issn

1527-3296

pii

S0196-6553(09)00006-6

journal_volume

37

pub_type

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