Abstract：
OBJECTIVE:The inflammatory cytokine interleukin-1 (IL-1) decreases mineralisation by immortalized mouse-derived cementoblastic cells (OC-CM cells), whilst various prostanoids, including fluprostenol (flup) increase it. Subtraction hybridisation conducted on flup minus IL-1-treated OC-CM cells revealed that one of the primary response genes preferentially induced by flup is the transcription factor Nur77. The objective of this study was to examine the signal transduction cascades regulating prostanoid induction of Nur77 gene expression in OC-CM cells. METHODS:Confluent OC-CM cells were treated with prostaglandin E(2) (PGE(2)), prostaglandin F(2alpha) (PGF(2alpha)), specific activators of the various EP prostanoid receptors and of the FP prostanoid receptor, and direct activators/inhibitors of the cyclic AMP-protein kinase A (PKA), protein kinase C (PKC) and intracellular calcium pathways. Nur77 gene expression was examined by mRNA extraction and Northern blot analysis. RESULTS:PGE(2) and PGF(2alpha) treatment of OC-CM cells significantly increased Nur77 mRNA expression in a time- and dose-dependent fashion. Both the EP1 prostanoid receptor-specific activator 16,16-dimethyl-PGE(2) and the FP prostanoid receptor-specific activator flup significantly increased Nur77 gene expression by OC-CM cells as compared to vehicle-treated controls. Increase in Nur77 gene expression was also observed when direct activators of the PKA, PKC and intracellular calcium pathways were used to treat OC-CM cells. Direct inhibition of the PKA, PKC and intracellular calcium pathways abrogated Nur77 gene expression induced by OC-CM cell treatment with PGE(2) and PGF(2alpha). CONCLUSION:Nur77 is a primary gene expressed by OC-CM cells and its induction appears to be mediated by the PKA, PKC and intracellular calcium pathways. Nur77 may affect expression of downstream target genes in OC-CM cells and partially regulate cementoblast cell function.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Moldovan SM,Nervina JM,Tetradis S,Camargo PMdoi
10.1016/j.archoralbio.2009.01.013subject
Has Abstractpub_date
2009-05-01 00:00:00pages
412-9issue
5eissn
0003-9969issn
1879-1506pii
S0003-9969(09)00032-6journal_volume
54pub_type
杂志文章abstract：:Salivary gland intralobular ducts are responsible for the modification of the electrolyte composition of the primary fluid secreted by the acini. However, the intracellular messengers that regulate this and other intralobular duct cell processes have not been fully characterized. To investigate the possibility that cA...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00028-3
更新日期：1996-07-01 00:00:00
abstract：:Bone morphogenetic protein-7 (BMP-7) affects differentiation of preosteoblasts enabling the resultant cells to respond optimally to acutely acting regulators. As the phosphoinositide cascade and, particularly, the calcium-mobilizing inositol 1,4,5-trisphosphate (InsP3) receptor are integral to stimulus-secretion coupl...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(99)00122-3
更新日期：2000-02-01 00:00:00
abstract：:Lymphocytes isolated from chronically-inflamed tissues were assessed for their ability to produce lymphokines without further stimulation in vitro. Cells were extracted from tissue obtained from 42 patients undergoing periodontal surgery. Cultures were set up in triplicate and supernatants collected after 48 h were as...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90094-9
更新日期：1985-01-01 00:00:00
abstract：OBJECTIVES:Dental evolution affects the geometry of the tooth, but the adaptive relevance of these changes is related to tooth sharpness, complexity, and relief (topography). On a set of laboratory mice, we assessed how wear related to age and food consistency affected molar geometry and topography. DESIGN:Three group...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.04.028
更新日期：2017-09-01 00:00:00
abstract：:The presence of human STRO-1(+)/CD146(+) periodontal ligament (PDL) cells has been reported, but obtaining a large amount of these cells is difficult. The purpose of this study was to evaluate the percentages of STRO-1(+)/CD146(+) cells in PDL cells and determine the effects of FGF-2 on the proliferation and multiline...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.12.003
更新日期：2012-06-01 00:00:00
abstract：:In adult miniature pigs (Sus scrofa) fibres of the masseter contract differentially, helping to produce the successive movements of the mandible during the chewing cycle. In infant pigs, however, most fibres of the masseter contract simultaneously. One hypothesis to explain the ontogenetic change in contraction patter...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90116-c
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to evaluate the pulpal changes associated with autogenous single-rooted immature tooth transplantation in dogs, using either one, or two-stage surgical techniques. METHODS:Teeth from 3 beagle dogs, 5 months old, were extracted and transplanted to mechanically prepared recipient ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.07.005
更新日期：2010-11-01 00:00:00
abstract：:Histatins 1, 3 and 5 are the major members of a histidine-rich protein family present in human salivary secretions. These proteins are distinct from many salivary proteins in their high positive charge density at neutral pH, and their antibacterial and antifungal properties. In this study, the hydroxyapatite adsorptio...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(03)00012-8
更新日期：2003-05-01 00:00:00
abstract：OBJECTIVE:Presently, a large number of individuals consider their companion animals as family members and have close contact with them in daily life. The purpose of the present study was to analyze the distribution of periodontopathic bacterial species in oral specimens taken from dogs and their owners. DESIGN:Dental ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.02.015
更新日期：2012-09-01 00:00:00
abstract：OBJECTIVE:Tooth eruption is a multifactorial process in which bone tissue plays a prevailing role. In this study we evaluated the bone overlying the developing tooth germ and the degree of tooth eruption of the first mandibular molar in pups born to mothers subjected to constant light during pregnancy. DESIGN:Pregnant...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2013.08.003
更新日期：2013-11-01 00:00:00
abstract：:The dental pulp is richly innervated by peptidergic nociceptive neurones that are of special interest because of their central role in dental pain and because they have some features that are not typical of other somatic nociceptors. Here, (35)S-riboprobes were used to determine whether pulpal afferents of adult (2-mo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00002-9
更新日期：2000-05-01 00:00:00
abstract：:The aim of this study was to investigate the age-related developmental changes of taste bud distribution within the subpopulations at different postnatal ages in the mouse oral cavity. Developmental changes of taste bud distribution on the soft palate, fungiform, foliate and circumvallate papillae in the mouse oral ca...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2008.01.005
更新日期：2008-06-01 00:00:00
abstract：:An electrophoretic method capable of analysing a small volume (5-10 microliter) of human palatine saliva without prior concentration was developed by combining the high resolution of isoelectric focusing in agarose with a highly sensitive silver protein staining method. Human palatine saliva exhibited 23 protein bands...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(83)90080-8
更新日期：1983-01-01 00:00:00
abstract：OBJECTIVE:Recent studies have showed that genetic factors involved in the host responses might determine the severity of periodontitis. hCAP-18/LL-37 is a part of the innate immune response in the oral cavity. The aim of the present study was to investigate the mutation of CAMP gene encoding hCAP-18/LL-37 in the patien...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.11.016
更新日期：2011-06-01 00:00:00
abstract：OBJECTIVE:The margin of a palatal cleft is a unique anatomical site since the palatal mucosa is continuous with the nasal or nasopharyngeal mucosa. The aim of this study was to compare the expression patterns of cytokeratins and basal membrane components of the mucosa in the area of the cleft. DESIGN:Biopsies from the...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.01.004
更新日期：2006-07-01 00:00:00
abstract：:Soluble reduced lysozyme was extensively digested by a trypsin-like protease purified from the culture supernatant of the bacterium. The digestion peptides were separated and purified by reversed-phase high-performance liquid chromatography, and were subjected to amino acid analysis. The fragments were identified by t...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90149-0
更新日期：1989-01-01 00:00:00
abstract：:Four-five months after the induction of diabetes, salivary tissues of male Wistar rats were preserved by glutaraldehyde fixation or rapid freezing in dry-ice cooled hexane. Fixed tissues were either processed and embedded for light and electron microscopy, or frozen and, together with unfixed tissues, sectioned and st...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(86)90021-x
更新日期：1986-01-01 00:00:00
abstract：OBJECTIVE:Syndecan-1 plays an important role in cell proliferation in dental papilla during tooth development. This study aimed to clarify the transcription mechanisms that regulate syndecan-1 gene expression in dental papilla. DESIGN:We analysed genomic conservation and putative transcriptional factor binding sites o...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.10.017
更新日期：2012-05-01 00:00:00
abstract：:The secretions (HPS) contained an arylamidase-like enzyme discovered by chromatography on Sephadex G-100 Superfine columns using N-L-alanyl-2-naphthylamine (2NA) as substrate. The enzyme was fractionated in the void volume, suggesting that its molecular weight was 150,000 or higher. It hydrolysed, with decreasing rate...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90050-0
更新日期：1985-01-01 00:00:00
abstract：:Close association between nerve terminals and preodontoblasts, odontoblasts and predentine was observed during healing after pulpotomy. The nerve terminals frequently contained large numbers of synaptic vesicles. Terminals with many vesicles tended to be fewer in the predentine than in the odontoblastic layer. The dis...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00018-k
更新日期：1995-07-01 00:00:00
abstract：OBJECTIVES:Calpains comprise a family of intracellular Ca2+-dependent cysteine proteases and are considered to play roles in various physiological phenomena with limited proteolytic activities against specific substrates. We herein revealed the expression and localization of calpain 3, the muscle-type calpain, in the s...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.05.019
更新日期：2016-10-01 00:00:00
abstract：OBJECTIVE:Receptor activator of nuclear factor-κB (RANK), RANK ligand (RANKL) and osteoprotegerin (OPG) are members of the superfamily of ligands and receptors of tumour necrosis factor family involved in bone metabolism. The formation, differentiation and activity of osteoclasts are regulated by these proteins. To cla...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2011.05.009
更新日期：2011-11-01 00:00:00
abstract：:Gingival crevicular fluid (GCF) was collected into capillary tubes from healthy gingiva and sites of advanced periodontitis. Following digestion with Pronase E, the glycosaminoglycans were isolated by successive precipitation into 5% cetylpyridinium chloride and 95% ethanol. Unsaturated disaccharide isomers of chondro...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00038-q
更新日期：1995-08-01 00:00:00
abstract：:The major gamma-carboxyglutamic acid-containing protein of rabbit cortical bone isolated and purified from near-neutral (pH 7.5) EDTA-extracts by DEAE-cellulose and Sephadex G 75 column chromatography had a molecular weight of about 5600 based on integral amino-acid composition; this was confirmed by high-performance ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(84)90149-3
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To investigate the mesial cervical concavity of maxillary first premolars and its relationship with root and canal configuration using cone-beam computed tomography (CBCT). DESIGN:Images of maxillary first premolars (n = 1056) were collected from patients (n = 601) who had undergonein vivo CBCT scanning. The...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.05.002
更新日期：2018-08-01 00:00:00
abstract：:A time-dependent, fundamental change in function for a sectorial tooth in a group of extinct, propleopine kangaroos is reported. In juvenile Ekaltadeta ima (Marsupialia, Hypsiprymnodontidae, Propleopinae) the second premolar (P2) functions as a serrated blade at the anterior end of the cheek tooth row. In adults, this...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00010-m
更新日期：1995-07-01 00:00:00
abstract：:The effects of dietary calcium deficiency and subsequent replenishment of the diet with calcium alone or with xylitol were studied. Thirty 3-week-old Wistar rats were labelled with an i.p. tetracycline injection. Twenty rats were fed a diet with 0.026% calcium (Ca-deficient); 10 received a 0.5% Ca diet (controls). Aft...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00036-o
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVE:The existence of extra-pineal melatonin has been observed in various tissues. No prior studies of melatonin in human oral mucosal tissue under the condition of chronic inflammation have been reported. The aim of this study was to investigate the presence of melatonin in oral mucosal tissue of patients with or...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.02.007
更新日期：2017-06-01 00:00:00
abstract：:The first indication of cartilage formation was detected on day 14.5 of pregnancy in close connection to the ossifying mandible. A separated blastema was never observed. Thus the findings support the concept that the condylar cartilage develops from already differentiated cells, not from primary undifferentiated mesen...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00105-0
更新日期：1996-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to analyze the characteristics of the clustered regularly interspaced short palindromic repeats (CRISPR) sites in 45 clinical Streptococcus mutans strains and their relationship to the clinical manifestations of early childhood caries (ECC). METHODS:Forty-five S. mutans strains were...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2017.07.023
更新日期：2017-11-01 00:00:00
