Abstract：
OBJECTIVES:Calpains comprise a family of intracellular Ca2+-dependent cysteine proteases and are considered to play roles in various physiological phenomena with limited proteolytic activities against specific substrates. We herein revealed the expression and localization of calpain 3, the muscle-type calpain, in the submandibular gland (SMG) of mice. DESIGN:The expression of the mRNA for conventional, ubiquitous calpains 1 and 2 and skeletal muscle-specific calpain 3 was examined in the major salivary glands of mice using RT-PCR, and the expression and localization of calpain 3 protein was examined in the SMG of mice using immunohistochemistry and Western blotting. RESULTS:The large catalytic subunits of calpains 1 and 2 and the small regulatory subunit common to calpains 1 and 2 were weakly expressed in the parotid gland, sublingual gland, and SMG at similar levels in males and females. In contrast, the single large catalytic subunit of calpain 3 was expressed predominantly in the SMG at markedly higher levels in males than in females and in a manner dependent on androgens. Immunoreactivity for calpain 3 was mainly localized in cells of the granular convoluted tubules (GCT) that developed preferentially in the male SMG. In GCT cells, calpain 3 immunoreactivity was localized predominantly in the cytosolic region and was absent in the secretory granules. CONCLUSIONS:These results revealed that the GCT is the primary site of production of calpain 3 in the mouse SMG.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Kumchantuek T,Nakata H,Sakulsak N,Yamamoto M,Iseki Sdoi
10.1016/j.archoralbio.2016.05.019subject
Has Abstractpub_date
2016-10-01 00:00:00pages
9-15eissn
0003-9969issn
1879-1506pii
S0003-9969(16)30138-8journal_volume
70pub_type
杂志文章abstract：OBJECTIVE:Accumulating evidence suggests an association between periodontitis and several systemic diseases, such as atherosclerosis. In the lesions of these diseases, nucleotide-binding domain leucine-rich repeat-containing protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase activation and ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2019.104625
更新日期：2020-02-01 00:00:00
abstract：:Close association between nerve terminals and preodontoblasts, odontoblasts and predentine was observed during healing after pulpotomy. The nerve terminals frequently contained large numbers of synaptic vesicles. Terminals with many vesicles tended to be fewer in the predentine than in the odontoblastic layer. The dis...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(95)00018-k
更新日期：1995-07-01 00:00:00
abstract：:The mineralization levels of erupted buccal enamel from 24 deciduous teeth were compared to those of 28 permanent teeth. Sections were prepared in a defined plane using a lapping machine which gave plano-parallel sections. Mineralization levels were recorded by quantitative microradiography at 25 equivalent anatomical...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90130-1
更新日期：1989-01-01 00:00:00
abstract：:The bioconcentration of lipophilic organochlorines in ovine dentine in comparison to adipose tissue was examined. Sheep were given a single dose (0.2-1.4 mg/kg body wt) of individual polychlorobiphenyl (PCB) congeners (tetrachlorobiphenyls IUPAC Nos. 54 and 80, and hexachlorobiphenyls IUPAC Nos. 155 and 169) and organ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(01)00079-6
更新日期：2001-12-01 00:00:00
abstract：OBJECTIVE:To investigate the effect of Rho kinase (ROCK) on Porphyromonas gingivalis outer membrane vesicles (OMVs)-induced suppression of endothelial nitric oxide synthase (eNOS) and explore the potential mechanism. DESIGN:Firstly, we investigated the effect of OMVs on total eNOS expression and eNOS activity in Human...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2014.12.009
更新日期：2015-03-01 00:00:00
abstract：:The accessory innervation of the mandibular dentition was examined by use of the retrograde transportation of horseradish peroxidase or wheat-germ agglutinin-horseradish peroxidase. Nerves within the cervical plexus which contained transported horseradish peroxidase included the great auricular and transverse cervical...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(93)90129-a
更新日期：1993-07-01 00:00:00
abstract：:There is growing evidence that the insulin-like growth factor (IGF) system plays an important role in the biology of oro-dento-facial tissues and organs, including the development, homeostasis and regeneration of the periodontium. To obtain basic data on the occurrence and distribution of IGF components in human perma...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2005.10.005
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:External apical root resorption is a pathologic consequence of orthodontic tooth movement. Cementum and dentin are removed from the root surface while active force is present. OBJECTIVE:The aim of this study was to identify and quantify extracellular matrix proteins, dentin matrix protein 1 (DMP1), dentin p...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2006.08.018
更新日期：2007-03-01 00:00:00
abstract：OBJECTIVE:This study aimed to evaluate the anti-caries effect of a fluoridated milk-based drink on a root caries model by assessing mineral loss and both biofilm microbial viability and acidogenicity under increasing concentrations of fluoride supplementation. DESIGN:Streptococcus mutans UA159 biofilms were grown on r...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104878
更新日期：2020-10-01 00:00:00
abstract：:Most authorities agree that the hydrodynamic theory of dentine sensitivity best explains the stimulus-response relations of most painful stimuli. However, as the usual hydrodynamic stimuli are so different, it has been impossible to compare them. The equivalency of hydrodynamic stimuli can be evaluated from measuremen...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00059-3
更新日期：1996-11-01 00:00:00
abstract：:Age-related changes in the content of non-reducible crosslink amino acids, pyridinoline and histidinoalanine, in rat mandibular bone were studied. The pyridinoline content markedly increased up to 12 months of age and thereafter slightly increased. The histidinoalanine content was low until 3 months of age, but therea...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(85)90075-5
更新日期：1985-01-01 00:00:00
abstract：:The fragmentation of foods (breakage function) was measured in five humans on "bagged' single particles of 28 foods from three food groups. The change in the square root of the specific surface of the particles (the specific surface being the area of particle silhouettes, measured by image analysis, divided by origina...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00102-1
更新日期：1997-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to evaluate the pulpal changes associated with autogenous single-rooted immature tooth transplantation in dogs, using either one, or two-stage surgical techniques. METHODS:Teeth from 3 beagle dogs, 5 months old, were extracted and transplanted to mechanically prepared recipient ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2010.07.005
更新日期：2010-11-01 00:00:00
abstract：:The purpose of this study was to determine maximum bite force in molar and incisor regions in young Brazilian indigenous individuals, who have had a natural diet since birth, and compare the sample with white Brazilian individuals. To do this, individuals were paired one-to-one (same weight, height, and Class I facial...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2007.10.003
更新日期：2008-03-01 00:00:00
abstract：:Biomechanical loads were varied by feeding diets of different consistencies. One group was fed hard diet for eight weeks, a second soft diet for eight weeks, and a third soft diet for four weeks followed by four weeks of hard diet. In all groups, alkaline-phosphatase activity was localized primarily in cells and extra...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(87)90043-4
更新日期：1987-01-01 00:00:00
abstract：:The release studied by incubating tissue slices in an enriched, oxygenated medium in the presence and absence of secretagogues indicated that slices from 1-day-old rats released K+ in response to carbamylcholine, but not in response to epinephrine. The response to carbamylcholine was dose-related, with a 12.6 +/- 1.3 ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(82)90100-5
更新日期：1982-01-01 00:00:00
abstract：:An outward flow of fluid through exposed dentine was demonstrated in anaesthetized cats. The flow was measured by observing the movement of the fat droplets of dilute milk in a glass capillary (i.d. 30 microns) with a microscope. The capillary was sealed to the dentine with a plastic cap. The resting flow rate through...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(92)90087-o
更新日期：1992-03-01 00:00:00
abstract：:In magnetic-stimulation studies where motor-evoked potentials (MEP) are measured on different occasions, accurate relocation of the stimulation site on the scalp is essential. Here a novel method of locating neural stimulation sites was tested and the reproducibility of MEPs in the human masseter assessed. The heads o...
journal_title：Archives of oral biology
pub_type： 临床试验,杂志文章
doi：10.1016/s0003-9969(98)00050-8
更新日期：1998-08-01 00:00:00
abstract：:The dental pulp is richly innervated by peptidergic nociceptive neurones that are of special interest because of their central role in dental pain and because they have some features that are not typical of other somatic nociceptors. Here, (35)S-riboprobes were used to determine whether pulpal afferents of adult (2-mo...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(00)00002-9
更新日期：2000-05-01 00:00:00
abstract：:Adhesion molecules are considered to have an active role in controlling cell differentiation, although the mechanisms involved have yet to be determined. The developing tooth provides an excellent model to use for determining the factors/processes regulating cell differentiation. The studies presented here focused spe...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/s0003-9969(96)00051-9
更新日期：1996-08-01 00:00:00
abstract：:Chronic snuff dipping has been associated with oral cancer in man and experimental animals. Here, the effects of a water-extract of snuff on the in-vitro development of human lymphokine-activated killer (LAK) activity were examined. The snuff extract inhibited both LAK cytotoxicity and DNA synthesis in a dose-dependen...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90064-7
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:Phosphoryl oligosaccharides of calcium (POs-Ca) are highly soluble calcium source made from potato starch. The aim of this study was to investigate the optimal concentrations of POs-Ca for the remineralization of subsurface enamel lesions in vitro. DESIGN:Demineralized bovine enamel slabs (n=5) were reminera...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2012.07.004
更新日期：2013-02-01 00:00:00
abstract：:A soluble trypsin-like enzyme (STE) was purified from a cell- and particle-free culture supernatant of this bacterium by a combination of ultra-centrifugation, ammonium-sulphate precipitation and gel-filtration chromatography on Sephacryl S-200. Trypsin-like activity in the culture supernatant was associated with a 58...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(88)90065-9
更新日期：1988-01-01 00:00:00
abstract：OBJECTIVE:To evaluate the effect of a fluoride toothpaste containing nano-sized sodium trimetaphosphate (TMPnano) on enamel demineralization in situ and composition of the biofilm. DESIGN:This crossover double-blind study consisted of four phases (seven days each) and 12 volunteers who wore oral appliances containing ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2018.09.019
更新日期：2018-12-01 00:00:00
abstract：:New protocols for regenerative endodontic treatment along with the parallel development of tissue engineering technologies are changing traditional knowledge and treatment possibilities for young patients with pulp-affected permanent immature teeth. The regeneration and completion of apical root development in patholo...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2012.11.008
更新日期：2013-03-01 00:00:00
abstract：:Salivary proteins and glycoproteins that participate in the formation of 2-h in vivo enamel pellicle were determined utilizing polyacrylamide gel electrophoresis [sodium dodecyl sulphate (SDS)-PAGE and anionic PAGE]/Western transfer analyses, and specific radiolabelling/SDS-PAGE fluorography. The sensitivity of these ...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(89)90070-8
更新日期：1989-01-01 00:00:00
abstract：:One of the most popular drinks worldwide, tea is rich in polyphenols and is beneficial to our health because it contributes to the prevention of many diseases. In the human oral cavity, there are more than 750 different species of bacteria living together within dental plaque. Some of the bacteria are pathogens that c...
journal_title：Archives of oral biology
pub_type： 杂志文章,评审
doi：10.1016/j.archoralbio.2019.03.027
更新日期：2019-06-01 00:00:00
abstract：:Experiments showed that the human capacity to maintain a particular isometric force in the presence of visual feedback during a force-level, pursuit-tracking experiment is less developed for the jaw-closing muscles than for the limb muscles. This finding may indicate that the projection on the trigeminal motoneurone p...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/0003-9969(91)90150-s
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:The objective was to determine the antibiofilm efficacy of Cymbopogon martinii and Thymus zygis essential oils and to estimate the disruption potential of oil-based endodontic irrigants on the multispecies biofilm formed in the root canals of extracted teeth. DESIGN:The essential oils were characterized (GC-...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2020.104842
更新日期：2020-09-01 00:00:00
abstract：OBJECTIVE:Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease characterized by permanent cartilage loss. Articular cartilage is maintained in a low-oxygen environment. The chondrocyte response to hypoxic conditions involves expression of hypoxia inducible factor 1α (HIF-1α), which induces chondroc...
journal_title：Archives of oral biology
pub_type： 杂志文章
doi：10.1016/j.archoralbio.2016.10.028
更新日期：2017-01-01 00:00:00
