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Involvement of reactive oxygen species in indomethacin-induced apoptosis of small intestinal epithelial cells.

Abstract：

BACKGROUND:The precise pathogenic mechanism of nonsteroidal antiinflammatory drug-induced small intestinal injury is still unknown. In the present study, we investigated the mechanism by which indomethacin induced mucosal injury by using an in vitro model of small intestine. METHODS:The colon cancer cell line Caco-2, exhibiting a small intestinal phenotype starting as a crypt cell and differentiating to a villous phenotype, and RIE, a rat intestinal epithelial cell line, were employed. Indomethacin was added to differentiated the Caco-2 and RIE monolayer, and cell death was quantified by MTT assay and LDH release in the cell culture supernatant. Indomethacin-induced cell death was also qualified by fluorescent probes under the fluorescent microscope. As a functional study, the permeability of the Caco-2 monolayer was assessed by measuring transepithelial electrical resistance (TEER) and the flux of FITC-conjugated dextran across the monolayer. Indomethacin-induced reactive oxygen species production in Caco-2 and RIE was evaluated by redoxsensitive fluorogenic probes using a fluorometer. In some experiments, antioxidants were used to clarify the role of reactive oxygen species on indomethacin-induced Caco-2 cell death. RESULTS:Indomethacin caused cell death (mainly apoptosis) of Caco-2 and RIE in a dose-and time-dependent manner that was correlated with increased permeability of the Caco-2 monolayer. Exposure of Caco-2 and RIE with indomethacin also resulted in a significant reactive oxygen species production that was inhibited by the pretreatment of these cells with antioxidants. CONCLUSIONS:Taken together, reactive oxygen species production is one of the mechanisms by which indomethacin induced small intestinal injury.

journal_name

J Gastroenterol

journal_title

Journal of gastroenterology

authors

Omatsu T,Naito Y,Handa O,Hayashi N,Mizushima K,Qin Y,Hirata I,Adachi S,Okayama T,Kishimoto E,Takagi T,Kokura S,Ichikawa H,Yoshikawa T

doi

10.1007/s00535-008-2293-3

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

30-4

eissn

0944-1174

issn

1435-5922

journal_volume

44 Suppl 19

pub_type

杂志文章

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Involvement of reactive oxygen species in indomethacin-induced apoptosis of small intestinal epithelial cells.