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TRPV4-like non-selective cation currents in cultured aortic myocytes.

Abstract：

:In this study, we provide evidence of critical changes in the expression of non-selective cation currents (NSCC) during culture in rat aortic myocytes. A selective TRPV4 agonist, 4alpha-phorbol 12,13-didecanoate (4alphaPDD), had little effect on membrane currents and intracellular Ca(2+) (Ca(2+)(i)) in freshly isolated cells from the aorta. In contrast, in cultured aortic myocytes with and without serum, 4alphaPDD at a concentration range between 0.3 and 3 muM effectively elevated Ca(2+)(i), which was abolished in the absence of external Ca(2+). Application of 4alphaPDD to cultured aortic myocytes also activated NSCC, which had a reversal potential of +3 mV. Both of these signals were blocked by ruthenium red (RuR), an effective blocker of TRPVs. Although the expression of TRPV4 mRNA transcript was found in cultured as well as non-cultured aortic myocytes, significant immunoreactivity to TRPV4 protein was only detected in cultured rat aortic myocytes. Moreover, cultured human pulmonary arterial smooth muscle cells (hPASM) had a substantial response to 4alphaPDD, which was susceptible to the removal of external Ca(2+) and application of RuR. These results provide a strong basis for our proposal that endogenous TRPV4 functions as an important regulator of Ca(2+)(i) in vascular myocytes under some physiological and pathophysiological conditions.

journal_name

J Pharmacol Sci

journal_title

Journal of pharmacological sciences

authors

Tanaka R,Muraki K,Ohya S,Yamamura H,Hatano N,Itoh Y,Imaizumi Y

doi

10.1254/jphs.08133fp

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

179-89

issue

2

eissn

1347-8613

issn

1347-8648

pii

JST.JSTAGE/jphs/08133FP

journal_volume

108

pub_type

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